OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-11969
![Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free [NBP2-11969]](https://resources.rndsystems.com/images/products/OX40-Ligand-TNFSF4-Antibody-MM0505-8S23-Flow-Cytometry-NBP2-11969-img0001.jpg "Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free [NBP2-11969]")
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Key Product Details
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Scientific Data Images for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free
Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free [NBP2-11969]
Flow Cytometry: OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) [NBP2-11969] - An intracellular stain was performed on U-937 cells withOX40 Ligand/TNFSF4 Antibody (MM0505-8S23) NBP2-11969PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.Applications for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free
Flow Cytometry
Immunohistochemistry
Immunohistochemistry-Frozen
Western Blot
Neutralization
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
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Background: OX40 Ligand/TNFSF4
Additional OX40 Ligand/TNFSF4 Products
Product Documents for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for OX40 Ligand/TNFSF4 Antibody (MM0505-8S23) - Azide and BSA Free
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Q: Could you clarify what 'guaranteed for this application on human samples' means? Can I return the antibody if it does not work for IHC-P in our hands?
A:
We stand behind our products 100% and that statement is refering to our Novus' Quality Guarantee.
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Q: I have bought a TNFSF4 antibody (MM0505-8S23) from you. Now I would like to be sure, that I reconstitute the lyophilized antibody the right way. Could you please tell me, with which fluid I am supposed to reconstitute it and what the final concentration should be?
A: To reconstitute our TNFSF4 antibody with catalogue number NBP2-11969 you should centrifuge the vial and then add 200ul sterile PBS. The final concentration is 500ug/ml. Reconstituted antibody can be aliquoted and stored frozen at -20 for at least for six months without detectable loss of activity. You should avoid repeated freeze-thaw cycles.
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Q: Could you clarify what 'guaranteed for this application on human samples' means? Can I return the antibody if it does not work for IHC-P in our hands?
A:
We stand behind our products 100% and that statement is refering to our Novus' Quality Guarantee.
-
Q: I have bought a TNFSF4 antibody (MM0505-8S23) from you. Now I would like to be sure, that I reconstitute the lyophilized antibody the right way. Could you please tell me, with which fluid I am supposed to reconstitute it and what the final concentration should be?
A: To reconstitute our TNFSF4 antibody with catalogue number NBP2-11969 you should centrifuge the vial and then add 200ul sterile PBS. The final concentration is 500ug/ml. Reconstituted antibody can be aliquoted and stored frozen at -20 for at least for six months without detectable loss of activity. You should avoid repeated freeze-thaw cycles.
Associated Pathways
