POLR2E Antibody (OTI3B5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-73518
![Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Western-Blot-NBP2-73518-img0002.jpg "Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]")
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3B5
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human POLR2E(NP_002686) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for POLR2E Antibody (OTI3B5) - Azide and BSA Free
Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]
Western Blot: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0012.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human pancreas tissue using anti-POLR2E mouse monoclonal antibody.![Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73518-img0004.jpg "Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-POLR2E antibody, and then analyzed by flow cytometry.![Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Western-Blot-NBP2-73518-img0001.jpg "Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]")
Western Blot: POLR2E Antibody (OTI3B5)Azide and BSA Free [NBP2-73518]
Western Blot: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0005.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Human tonsil.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0006.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR2E mouse monoclonal antibody.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0007.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR2E mouse monoclonal antibody.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0008.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR2E mouse monoclonal antibody.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0009.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR2E mouse monoclonal antibody.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0010.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human breast tissue using anti-POLR2E mouse monoclonal antibody.![Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73518-img0011.jpg "Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Immunohistochemistry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Staining of paraffin-embedded Human lymphoma tissue using anti-POLR2E mouse monoclonal antibody.![Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]](https://resources.rndsystems.com/images/products/POLR2E-Antibody-OTI3B5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73518-img0003.jpg "Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]")
Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518]
Flow Cytometry: POLR2E Antibody (OTI3B5) - Azide and BSA Free [NBP2-73518] - Analysis of Jurkat cells, using anti-POLR2E antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for POLR2E Antibody (OTI3B5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:500-2000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: POLR2E
Alternate Names
DNA directed RNA polymerase II 23 kda polypeptide, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, polymerase (RNA) II (DNA directed) polypeptide E (25kD), polymerase (RNA) II (DNA directed) polypeptide E, 25kDa, RPABC1, RPB5, RPB5 homolog, XAP4RNA polymerases I, II, and III subunit ABC1
Gene Symbol
POLR2E
Additional POLR2E Products
Product Documents for POLR2E Antibody (OTI3B5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for POLR2E Antibody (OTI3B5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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