Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 896032
View all CXCR7/RDC-1 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR7/RDC-1
Met1-Lys362
Accession # NP_445804

Specificity

Stains rat CXCR7/RDC-1 transfectants but not irrelevant transfectants in flow cytometry. 

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Rat CXCR7/RDC‑1 Antibody

Detection of CXCR7/RDC-1 antibody in HEK293 Human Cell Line Transfected with Rat CXCR7/RDC-1 and eGFP antibody by Flow Cytometry.

Detection of CXCR7/RDC‑1 in HEK293 Human Cell Line Transfected with Rat CXCR7/RDC-1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) rat CXCR7/RDC-1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB8399) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Applications for Rat CXCR7/RDC‑1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with rat CXCR7/RDC-1 and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR7/RDC-1

CXCR7 (CXC Chemokine Receptor 7), also known as GPRN1, RDC-1 and Chemokine Orphan Receptor 1, is a 60 kDa member of the G-protein coupled receptor 1 family. It is expressed on multiple cell types, including neurons, T cells, NK cells, neutrophils, B cells plus angiogenic endothelial cells. CXCR7 forms both homodimers and heterodimers with CXCR4. It selectively binds I-TAC and SDF-1, and appears to involve beta -Arrestin 2 during signaling. Notably, a CXCR7:CXCR4 heterodimer shows increased responsiveness to SDF-1, and I-TAC may actually block some SDF-1-mediated migration activity.

Alternate Names

ACKR3, CMKOR1, CXCR7, GPR159, RDC-1

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Gene Symbol

ACKR3

UniProt

NP_445804

Additional CXCR7/RDC-1 Products

Product Documents for Rat CXCR7/RDC‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat CXCR7/RDC‑1 Antibody

For research use only

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