Rat VEGF 164 Antibody [Biotin]

R&D Systems | Catalog # BAF564

R&D Systems

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Binding Assay, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all VEGF Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat VEGF164 (R&D Systems, Catalog # 564-RV)
Ala27-Arg190
Accession # AAL07526

Specificity

Detects rat VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat VEGF 164 Antibody [Biotin]

Rat VEGF ELISA Standard Curve

Rat VEGF ELISA Standard Curve

Recombinant Rat VEGF 164 (Catalog # 564-RV) was serially diluted and captured by Mouse Anti-Rat VEGF 164 Monoclonal Antibody (Catalog # MAB564) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF564) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Rat VEGF 164 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample:

Recombinant Rat VEGF164 (Catalog # 564-RV)

Recombinant Mouse VEGF164 (Catalog # 493-MV

Rat VEGF Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Rat VEGF164 Antibody (Catalog # MAB564)
  • Standard: Recombinant Rat VEGF 164 Protein (Catalog # 564-RV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Rat VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 90% with feline and equine, and 89% with canine and porcine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

AAL07526

Additional VEGF Products

Product Documents for Rat VEGF 164 Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for Rat VEGF 164 Antibody [Biotin]

For research use only

Citations for Rat VEGF 164 Antibody [Biotin]

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