rRNA Antibody (Y10b) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80932

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, All Species, Bacteria

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # Y10b

Format

Azide and BSA Free
View all rRNA Primary Antibodies »

Product Specifications

Immunogen

The whole 5.8S ribosomal RNA.

Reactivity Notes

This antibody reacts with all eukaryotes. Bacteria reactivity reported in scientific literature (PMID: 26134566).

Localization

Cytoplasmic. Nuclear.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for rRNA Antibody (Y10b) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Staining of rRNA in vero cells. Image from the standard format of this antibody.
Immunohistochemistry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunohistochemistry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunohistochemistry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review. Image from the standard format of this antibody.
Flow Cytometry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Flow Cytometry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Flow Cytometry: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were directly conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rRNA (Y10b) conjugated to DyLight 488 [NB100-662G] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/ Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932]

Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.
CyTOF-ready: rRNA Antibody (Y10b) - Azide and BSA Free [NBP2-80932] - Human bone marrow after RBC lysis, stained with anti-CD16, fixed, permeabilized with methanol, stained with anti-lactoferrin 1C6 NB120-10109 and anti-rRNA Y10b NB100-662, and gated on myeloid cells per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review. Image from the standard format of this antibody.

Applications for rRNA Antibody (Y10b) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2500

Immunoprecipitation

10 ul/lysate
Application Notes
This rRNA Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: rRNA

rRNA (ribosomal RNA) forms a major structural component of ribosomes which provides a mechanism for decoding mRNA information into amino acids/proteins through the process of translation. rRNA plays a key role in accurate positioning of mRNA as well as peptidyl tRNA, and also plays a structural role in peptidyl transferase activity by generating scaffolds where ribosomal proteins are assembled. rRNA is generated inside nucleolus, which is localized in nucleus of the cells and contains genes essential for encoding rRNA. Defective rRNA may implicate in certain pathologies, for example: decreased rRNA expression has been observed in subjects with myelodysplastic syndromes, and abnormal rRNA expression has also been linked to Alzheimer's disease. Our rRNA (Y10b) antibody recognizes both the large as well as the small subunit of rRNA from all eukaryotic cells and with is applicability in ICC/IF and IP techniques, it provides a valuable molecular tool for rRNA research.

Alternate Names

ribosomal RNA 5.8s

Additional rRNA Products

Product Documents for rRNA Antibody (Y10b) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for rRNA Antibody (Y10b) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for rRNA Antibody (Y10b) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review rRNA Antibody (Y10b) - Azide and BSA Free and earn rewards!

Have you used rRNA Antibody (Y10b) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies