Smad7 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NB100-56688
![Western Blot: Smad7 AntibodyAzide Free [NB100-56688]](https://resources.rndsystems.com/images/products/Smad7-Antibody---Azide-Free-Western-Blot-NB100-56688-img0004.jpg "Western Blot: Smad7 AntibodyAzide Free [NB100-56688]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Sheep
Cited:
Mouse
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Smad7 Antibody - Azide and BSA Free
Western Blot: Smad7 AntibodyAzide Free [NB100-56688]
Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody. Image using the standard format of this product.![Immunohistochemistry-Paraffin: Smad7 Antibody - Azide Free [NB100-56688]](https://resources.rndsystems.com/images/products/Smad7-Antibody---Azide-Free-Immunohistochemistry-Paraffin-NB100-56688-img0003.jpg "Immunohistochemistry-Paraffin: Smad7 Antibody - Azide Free [NB100-56688]")
Immunohistochemistry-Paraffin: Smad7 Antibody - Azide Free [NB100-56688]
Immunohistochemistry-Paraffin: Smad7 Antibody - Azide Free [NB100-56688] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz. Image using the standard format of this product.![Western Blot: Smad7 AntibodyAzide Free [NB100-56688]](https://resources.rndsystems.com/images/products/Smad7-Antibody---Azide-Free-Western-Blot-NB100-56688-img0002.jpg "Western Blot: Smad7 AntibodyAzide Free [NB100-56688]")
Western Blot: Smad7 AntibodyAzide Free [NB100-56688]
Western Blot: Smad7 Antibody - Azide Free [NB100-56688] - Analysis using SMAD7 antibody. Lysate from A549 cells probed with SMAD7 antibody at 5 ug/ml.Applications for Smad7 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
4 - 6 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Sterile - filtered PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Smad7
Long Name
Mothers Against DPP Homolog 7
Alternate Names
MADH7, MADH8
Gene Symbol
SMAD7
Additional Smad7 Products
Product Documents for Smad7 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Smad7 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Smad7 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
