Smad7 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24710

Novus Biologicals

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Sheep

Cited:

Human, Mouse, Ovine

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot, ELISA, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Smad7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against two peptides made from within amino acids 10 and 50 of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24576683).

Specificity

This antibody may cross-react with SMAD6 depending on cell or tissue-specific expression.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad7 Antibody - BSA Free

Western Blot: Smad7 Antibody [NBP2-24710]

Western Blot: Smad7 Antibody [NBP2-24710]

Smad7-Antibody-Western-Blot-NBP2-24710-img0005.jpg
Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-24710] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human liver using Smad7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Smad7 was observed. Staining was performed by Histowiz.
Western Blot: Smad7 Antibody [NBP2-24710]

Western Blot: Smad7 Antibody [NBP2-24710]

Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using SMAD7 antibody. Lysate from HepG2 cells probed with SMAD7 antibody.
Western Blot: Smad7 Antibody [NBP2-24710]

Western Blot: Smad7 Antibody [NBP2-24710]

Western Blot: Smad7 Antibody [NBP2-24710] - Analysis using the Azide Free version of NBP2-24710. Detection of lysate from A549 cells probed with SMAD7 antibody.

Applications for Smad7 Antibody - BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 32108968)

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad7

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

Long Name

Mothers Against DPP Homolog 7

Alternate Names

MADH7, MADH8

Gene Symbol

SMAD7

UniProt

NP_005895 (Human)

Additional Smad7 Products

Product Documents for Smad7 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Smad7 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Smad7 Antibody - BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail