Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline, Goat, Primate, Sheep

Cited:

Human, Mouse, Ovine

Predicted:

Bovine (93%), Chimpanzee (93%), Equine (100%), Monkey (100%), Opossum (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Simple Western

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOX9 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 225-275 of human SOX9 was used as the immunogen for this antibody.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 29624608). The amino acid sequence used as immunogen for the ZBTB2 antibody is 100% homologous in monkey, opossum and horse, and 93% homologous in cow and chimpanzee.

Marker

Sertoli Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX9 Antibody - BSA Free

Simple Western: SOX9 Antibody [NBP2-24659]

Simple Western: SOX9 Antibody [NBP2-24659]

Simple Western: SOX9 Antibody [NBP2-24659] - Simple Western lane view shows a specific band for SOX9 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: SOX9 Antibody [NBP2-24659]

Immunohistochemistry: SOX9 Antibody [NBP2-24659]

Immunohistochemistry: SOX9 Antibody [NBP2-24659] - Osteo-chondroprogenitor cells in the zone adjacent to the titanium plate surface are positive for Sox9 (arrows). Picture on the left represent Group 0.1, pictures on the right represent Group 0.6. 100-fold (c-d) magnification. PLoS One. 2018 Apr 6;13(4):e0195466. doi: 10.1371/journal.pone.0195466.
Immunohistochemistry: SOX9 Antibody [NBP2-24659]

Immunohistochemistry: SOX9 Antibody [NBP2-24659]

SOX9-Antibody-Immunohistochemistry-NBP2-24659-img0008.jpg
Immunohistochemistry-Frozen: SOX9 Antibody [NBP2-24659]

Immunohistochemistry-Frozen: SOX9 Antibody [NBP2-24659]

Immunohistochemistry-Frozen: SOX9 Antibody [NBP2-24659] - Feline optic nerve tissue section was incubated with antibody at 1:200 and further stained with Alexa secondary antibody. Image was captured with an epifluorescence microscope. This image was submitted via customer review.
Western Blot: SOX9 Antibody [NBP2-24659]

Western Blot: SOX9 Antibody [NBP2-24659]

Western Blot: SOX9 Antibody [NBP2-24659] - Analysis of SOX9 in mouse embryo brain lysate in the 1) absence and 2) presence of immunizing peptide using SOX9 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: SOX9 Antibody [NBP2-24659]

Western Blot: SOX9 Antibody [NBP2-24659]

Western Blot: SOX9 Antibody [NBP2-24659] - Analysis of SOX9 in human brain lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain lysate using SOX9 antibody at 3 ug/ml.
Immunohistochemistry-Paraffin: SOX9 Antibody [NBP2-24659]

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP2-24659]

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP2-24659] - Analysis of SOX9 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top left) and SOX9 antibody (bottom left, right) at 5 ug/ml.
SOX9 Antibody

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP2-24659] -

Immunohistochemistry-Paraffin: SOX9 Antibody [NBP2-24659] - 3′‐Sialyllactose regulates anabolic & catabolic factor expression via Erk & NF‐ kappa B pathways. (A, B) Chondrocytes were pre‐treated with different concentrations of 3′‐sialyllactose for 24 hrs prior to treatment with IL‐1 beta (5 ng/ml) for 10 min. Erk phosphorylation was measured by Western blotting (A, upper panel) & densitometry (A, lower panel). The protein level of I kappa B was detected by Western blotting (B, upper panel) & densitometry (B, lower panel). Erk was detected as a loading control. (C) Immunohistochemical staining of Sox9, I kappa B & pErk in DMM‐induced OA mice with administration of PBS & 3′‐sialyllactose. (D) Schematic diagram of the signalling pathway leading to Sox9 activation & NF‐ kappa B inhibition by 3′‐sialyllactose. Values represent the means ± S.E.M. *P < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28782172), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SOX9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

2-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 51 kDa.

Reviewed Applications

Read 1 review rated 3 using NBP2-24659 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

UniProt

P48436

Additional SOX9 Products

Product Documents for SOX9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOX9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SOX9 Antibody - BSA Free

Customer Reviews for SOX9 Antibody - BSA Free (1)

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  • SOX9 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Optic nerve
    Species: Feline
    Verified Customer | Posted 03/09/2018
    Feline optic nerve tissue section was incubated with antibody at 1:200 and further stained with Alexa secondary antibody. Image was captured with an epifluorescence microscope.
    SOX9 Antibody - BSA Free NBP2-24659

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