SSX1 Antibody (OTI1E10)

Novus Biologicals | Catalog # NBP2-00614

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E10
View all SSX1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SSX1 (NP_005626) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSX1 Antibody (OTI1E10)

Western Blot: SSX1 Antibody (OTI1E10) [NBP2-00614]

Western Blot: SSX1 Antibody (OTI1E10) [NBP2-00614]

Western Blot: SSX1 Antibody (OTI1E10) [NBP2-00614] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1.
Immunocytochemistry/ Immunofluorescence: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunocytochemistry/ Immunofluorescence: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunocytochemistry/Immunofluorescence: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1.
Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Human lymph node tissue using anti-SSX1 mouse monoclonal antibody.
Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614]

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614]

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSX1 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSX1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614]

Immunohistochemistry-Paraffin: SSX1 Antibody (OTI1E10) [NBP2-00614] - Staining of paraffin-embedded Human Kidney tissue using anti-SSX1 mouse monoclonal antibody.
Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614]

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614]

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - Analysis of Hela cells, using anti-SSX1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614]

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614]

Flow Cytometry: SSX1 Antibody (OTI1E10) [NBP2-00614] - Analysis of Jurkat cells, using anti-SSX1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for SSX1 Antibody (OTI1E10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.64 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SSX1

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. [provided by RefSeq].

Alternate Names

Cancer/testis antigen 5.1, CT5.1cancer/testis antigen family 5, member 1, MGC150425, protein SSX1, sarcoma, synovial, X-chromosome-related 1, SSRC, synovial sarcoma, X breakpoint 1MGC5162

Entrez Gene IDs

6756 (Human)

Gene Symbol

SSX1

Additional SSX1 Products

Product Documents for SSX1 Antibody (OTI1E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SSX1 Antibody (OTI1E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SSX1 Antibody (OTI1E10)

Customer Reviews for SSX1 Antibody (OTI1E10)

There are currently no reviews for this product. Be the first to review SSX1 Antibody (OTI1E10) and earn rewards!

Have you used SSX1 Antibody (OTI1E10)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies