STK39 Antibody (OTI4E3) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74406

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4E3

Format

Azide and BSA Free
View all STK39 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human STK39 (NP_037365) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

59.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STK39 Antibody (OTI4E3) - Azide and BSA Free

Western Blot: STK39 Antibody (OTI4E3)Azide and BSA Free [NBP2-74406]

Western Blot: STK39 Antibody (OTI4E3)Azide and BSA Free [NBP2-74406]

Western Blot: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STK39 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STK39.
Immunocytochemistry/ Immunofluorescence: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunocytochemistry/ Immunofluorescence: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunocytochemistry/Immunofluorescence: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - Staining of COS7 cells using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - Staining of paraffin-embedded Human endometrium tissue using anti-STK39 mouse monoclonal antibody.
Flow Cytometry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Flow Cytometry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Flow Cytometry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-STK39 antibody, and then analyzed by flow cytometry.
Western Blot: STK39 Antibody (OTI4E3)Azide and BSA Free [NBP2-74406]

Western Blot: STK39 Antibody (OTI4E3)Azide and BSA Free [NBP2-74406]

Western Blot: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-STK39 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STK39 mouse monoclonal antibody.
Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406]

Immunohistochemistry: STK39 Antibody (OTI4E3) - Azide and BSA Free [NBP2-74406] - Staining of paraffin-embedded Human Ovary tissue using anti-STK39 mouse monoclonal antibody.

Applications for STK39 Antibody (OTI4E3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-1000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STK39

STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Alternate Names

DCHTsmall intestine SPAK-like kinase, DKFZp686K05124, EC 2.7.11, EC 2.7.11.1, PASK, proline-alanine-rich STE20-related kinase, serine threonine kinase 39, Serine/threonine-protein kinase 39, SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast), STE20/SPS1 homolog, STE20/SPS1-related proline-alanine-rich protein kinase, Ste20-like protein kinase, Ste-20-related kinase

Gene Symbol

STK39

Additional STK39 Products

Product Documents for STK39 Antibody (OTI4E3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STK39 Antibody (OTI4E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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