UQCRFS1 Antibody (JG62-31)
Novus Biologicals | Catalog # NBP2-75697
Recombinant Monoclonal Antibody
![Western Blot: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Western-Blot-NBP2-75697-img0010.jpg "Western Blot: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JG62-31 expressed in HEK293
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Product Specifications
Immunogen
Recombinant protein within Human UQCRFS1 aa 180-274 / 274. (SwissProt: P47985 Human; SwissProt: Q9CR68 Mouse; SwissProt: P20788 Rat)
Localization
Mitochondrion.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for UQCRFS1 Antibody (JG62-31)
Western Blot: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Western Blot: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of UQCRFS1 on different lysates using anti-UQCRFS1 antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: 293,Lane 3: Mouse kidney![Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunocytochemistry-Immunofluorescence-NBP2-75697-img0004.jpg "Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunocytochemistry/Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Staining UQCRFS1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunohistochemistry-Paraffin-NBP2-75697-img0009.jpg "Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded rat skeletal muscle tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.![Flow Cytometry: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Flow-Cytometry-NBP2-75697-img0001.jpg "Flow Cytometry: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Flow Cytometry: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Flow Cytometry: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.![Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunocytochemistry-Immunofluorescence-NBP2-75697-img0002.jpg "Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunocytochemistry/Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Staining UQCRFS1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunocytochemistry-Immunofluorescence-NBP2-75697-img0003.jpg "Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunocytochemistry/ Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunocytochemistry/Immunofluorescence: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Staining UQCRFS1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunohistochemistry-Paraffin-NBP2-75697-img0005.jpg "Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded human colon cancer tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunohistochemistry-Paraffin-NBP2-75697-img0006.jpg "Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded human appendix tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunohistochemistry-Paraffin-NBP2-75697-img0007.jpg "Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded human kidney tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]](https://resources.rndsystems.com/images/products/UQCRFS1-Antibody-JG62-31-Immunohistochemistry-Paraffin-NBP2-75697-img0008.jpg "Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]")
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697]
Immunohistochemistry-Paraffin: UQCRFS1 Antibody (JG62-31) [NBP2-75697] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-UQCRFS1 antibody. Counter stained with hematoxylin.Applications for UQCRFS1 Antibody (JG62-31)
Application
Recommended Usage
Flow Cytometry
1:10-1:50
Immunocytochemistry/ Immunofluorescence
1:500-1:2000
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UQCRFS1
Alternate Names
Complex III subunit 5, Cytochrome b-c1 complex subunit 5, cytochrome b-c1 complex subunit Rieske, mitochondrial, EC 1.10.2.2, Rieske iron-sulfur protein, RIP1, RIS1, RISPUbiquinol-cytochrome c reductase iron-sulfur subunit, ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, UQCR5
Gene Symbol
UQCRFS1
Additional UQCRFS1 Products
Product Documents for UQCRFS1 Antibody (JG62-31)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UQCRFS1 Antibody (JG62-31)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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