WT1 Antibody (CL13165) - BSA Free

Novus Biologicals | Catalog # NBP3-18564

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13165

Format

BSA Free
View all WT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P19544, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse WT1 Antibody (CL13165) - BSA Free (NBP3-18564) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for WT1 Antibody (CL13165) - BSA Free

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human fallopian tube and lymph node tissues using NBP3-18564 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.
Western Blot: WT1 Antibody (CL13165) [NBP3-18564]

Western Blot: WT1 Antibody (CL13165) [NBP3-18564]

Western Blot: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564]

Immunocytochemistry/ Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564]

Immunocytochemistry/Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human lymph node shows no positivity in germinal center cells as expected.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564]

Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.

Applications for WT1 Antibody (CL13165) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WT1

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Long Name

Wilms Tumor 1

Alternate Names

GUD, WAGR, WIT-2, WT33

Gene Symbol

WT1

Additional WT1 Products

Product Documents for WT1 Antibody (CL13165) - BSA Free

Certificate of Analysis

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Product Specific Notices for WT1 Antibody (CL13165) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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