Adoptive Cell Transfer: Investigate CAR Signaling

Chimeric antigen receptor (CAR) T cell transfer has had success as a treatment for leukemia and lymphoma, but solid tumors have been more challenging due to the rarity of true tumor-specific target molecules and the immunosuppressive nature of the tumor microenvironment. A better understanding of CAR signaling pathways and how they differ from T cell receptor (TCR) signaling pathways is needed to better inform future CAR-based strategies targeting solid tumors. For example, what aspects of endogenous TCR signaling are missing? Is the cytokine secretion profile different between CAR T cells and conventional T cells? Our Proteome Profiler Antibody Arrays are ideally suited to address questions like these by providing an unbiased analysis of both intracellular and extracellular T cell responses.

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Proteome Profiler Antibody Arrays

Our membrane-based antibody arrays require no specialized equipment and eliminate the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high-quality capture antibodies. The arrays utilize chemiluminescence for detection and membranes can be assessed for protein levels in the same manner as traditional Western blots. Our antibody arrays are widely cited in the primary literature, highlighting their utility and popularity.

Analyze Intracellular Signaling

Quickly compare and contrast signaling downstream of CARs and conventional TCRs to help direct more effective CAR design strategies. Our Phospho-specific Proteome Profiler Antibody Arrays allow you to assess the phosphorylation status of up to 59 signaling molecules simultaneously.

View Larger Signal Transduction Profile of Leukemia Cells. Jurkat human acute T cell leukemia cells were untreated or treated with 2 mM H2O2 for 2 minutes. Cell lysates were analyzed using the Human Phospho-Kinase Array Kit (Catalog # ARY003B).
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Monitor Cytokine Secretion Profiles

Identify similarities and differences between the cytokine secretion profiles of CAR T cells and conventional T cells using our Proteome Profiler Antibody Arrays to learn more about CAR T cell responses. Simultaneously measure the expression levels of up to 111 cytokines to save sample and time.

Figure 1C
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Cytokine Secretion Profile of Leukemia Cells. THP-1 human acute monocytic leukemia cells were untreated or treated with 1 µg/mL of Recombinant Human IFN-gamma (Catalog # 285-IF) for 16 hours. Cell supernates were analyzed using the Human XL Cytokine Array Kit (Catalog # ARY022).
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