Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of human protein kinase phosphorylation. Validated for analyte detection in cell lysates.

Key Benefits

  • Detects phosphorylation of 43 human kinases and total amounts of 2 related proteins simultaneously
  • Requires no specialized equipment
 

 

Principle of the Assay

The Proteome Profiler Human Phospho-Kinase Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Phosphorylation of captured proteins is detected with biotinylated phospho-specific detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount phosphorylation in the bound analyte.

Why Use an Antibody Array to Detect Receptor Phosphorylation?

Determining the phosphorylation of multiple kinases in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple phosphorylations in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents

Each Human Phospho-Kinase Antibody Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized equipment.

  • 8 Array Membranes (4 of membrane A and 4 of membrane B)
  • 8-Well Multi-dish
  • Lysis Buffer
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail A
  • Detection Antibody Cocktail B
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed product datasheet with protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2°C to 8°C. Do not use past kit expiration date.

 
Simultaneously detect the relative phosphorylation of these proteins in a single sample.
Akt 1/2/3 (S473) Hck (Y411) PRAS40 (T246)
Akt 1/2/3 (T308) HSP27 (S78/S82) Pyk2 (Y402)
AMPK alpha1 (T183) HSP60 RSK1/2/3 (S380)
AMPK alpha2 (T172) JNK 1/2/3 (T183/Y185, T221/Y223) Src (Y419)
beta-Catenin Lck (Y394) STAT2 (Y689)
Chk-2 (T68) Lyn (Y397) STAT3 (S727)
c-Jun (S63) MSK1/2 (S376/S360) STAT3 (Y705)
CREB (S133) p27 (T198) STAT5a (Y699)
EGF R (Y1086) p38 alpha (T180/Y182) STAT5a/b (Y699)
eNOS (S1177) p53 (S15) STAT5b (Y699)
ERK1/2 (T202/Y204, T185/Y187) p53 (S392) STAT6 (Y641)
FAK (Y397) p53 (S46) TOR (S2448)
Fgr (Y412) p70 S6 Kinase (T421/S424) WNK-1 (T60)
Fyn (Y420) PDGF R beta (Y751) Yes (Y426)
GSK-3 alpha/beta (S21/S9) PLC gamma-1 (Y783)  

 

Assays for analytes represented in the Human Phospho-Kinase Array Kit

 

Analyte DuoSet® IC ELISA Development Systems (Total) DuoSet® IC ELISA
Development Systems (Phospho)
Surveyor™ IC or Quantikine®
ELISA Kits
Cell-based
ELISA Kits
Akt (S473)   DYC887B SUV887B  
Akt (T308)        
AMPK alpha1 (T183) DYC3197 DYC3528    
AMPK alpha2 (T172)        
beta-Catenin DYC1329   DYC1329  
Chk2 (T68)   DYC1626    
c-Jun (S63)        
CREB (S133)   DYC2510    
EGF R (Y1086) DYC1854   DEGFR0  
eNOS (S1177)        
ERK1/2 (T202/Y204, T185/Y187)   DYC1018B    
FAK (Y397) DYC4467      
Fgr (Y412)        
Fyn (Y420)        
GSK-3 alpha/beta (S21/S9) DYC2157 DYC2630   KCB1590
Hck (Y411)        
HSP27 (S78/S82) DYC1580 DYC2314   KCB2314
HSP60 DYC1800      
JNK pan (T183/Y185, T221/Y223) DYC1205 DYC1387    
Lck (Y394)        
Lyn (Y397)   DYC3936    
MSK1/2 (S376/S360)        
p27 (T198) DYC2256      
p38 alpha (T180/Y182) DYC8691B DYC869B    
p53 (S15) DYC1043 DYC1839    
p53 (S46) DYC1043 DYC1489    
p53 (S392) DYC1043 DYC2996    
p70 S6 Kinase (T389)   DYC896    
p70 S6 Kinase (T421/S424) DYC8962 DYC8965    
PDGF R beta (Y751) DYC385 DYC3096    
PLC gamma-1 (Y783)        
PRAS40 (T246)   DYC6890    
PYK2 (Y402)        
RSK1/2/3 (S380/S386/S377)   DYC889B    
Src (Y419)   DYC2685    
STAT2 (Y689)        
STAT3 (S727)        
STAT3 (Y705)   DYC4607B    
STAT5a (Y694)        
STAT5a/b (Y694/Y699)        
STAT5b (Y699)        
STAT6 (Y641)        
TOR (S2448)   DYC1665    
WNK-1(T60)   DYC4720    
Yes (Y426)   DYC3929    

Troubleshooting Guide

Data Examples
Figure 1.
View Larger Image

Figure 1. MCF-7 human breast cancer cells were either left untreated or exposed to 50 J/m2 of UV light followed by a 4 hour recovery before lysis.

Figure 2.
View Larger Image

Figure 2. A431 human epithelial carcinoma cells were either left untreated or treated with 200 ng/mL recombinant human (rh) EGF (Catalog # 236-EG) for 5 minutes.

Figure 3.
View Larger Image

Figure 3. MCF-7 human breast cancer cells were either left untreated or treated with 100 ng/mL of rhIGF-I (Catalog # 291-G1) for 1 hour.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Kinase Assay Kits
Protein kinases are the largest class of enzymes in the human genome. These enzymes regulate almost all cellular processes by adding phosphate groups to proteins, thereby modifying the activity, localization, and overall function of their targets. Consequently, abnormal activity of kinases underlies many diseases including cancer.
  • Alternate Names:
    Kinase Assay Kits
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

130 Citations: Showing 1 - 10
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Species
Sample Type
  1. Resistance to the antibody-drug conjugate T-DM1 is based in a reduction in lysosomal proteolytic activity
    Authors: C Ríos-Luci, S García-Alo, E Díaz-Rodrí, M Nadal-Serr, J Arribas, A Ocaña, A Pandiella
    Cancer Res., 2017;0(0):. 2017
  2. Secretory phospholipase A2 modified HDL rapidly and potently suppresses platelet activation
    Authors: S Curcic, M Holzer, L Pasterk, E Knuplez, TO Eichmann, S Frank, R Zimmermann, R Schicho, A Heinemann, G Marsche
    Sci Rep, 2017;7(1):8030. 2017
  3. Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients.
    Authors: Banko Z, Pozsgay J, Szili D, Toth M, Gati T, Nagy G, Rojkovich B, Sarmay G
    J Immunol, 2017;198(4):1512-1520. 2017
  4. EpEX/EpCAM and Oct4 or Klf4 alone are sufficient to generate induced pluripotent stem cells through STAT3 and HIF2�
    Authors: II Kuan, KH Liang, YP Wang, TW Kuo, YJ Meir, SC Wu, SC Yang, J Lu, HC Wu
    Sci Rep, 2017;7(0):41852. 2017
  5. Ras-MEK Signaling Mediates a Critical Chk1-dependent DNA Damage Response in Cancer Cells
    Authors: HJ Lee, Y Cao, V Pham, E Blackwood, C Wilson, M Evangelist, C Klijn, D Stokoe, J Settleman
    Mol. Cancer Ther, 2017;0(0):. 2017
  6. Activation of p38 and Erk Mitogen-Activated Protein Kinases Signaling in Ocular Rosacea
    Authors: EJ Wladis, S Swamy, A Herrmann, J Yang, JA Carlson, AP Adam
    Invest. Ophthalmol. Vis. Sci, 2017;58(2):843-848. 2017
  7. Ectodomain Shedding of the Cell Adhesion Molecule Nectin-4 in Ovarian Cancer is Mediated by ADAM10 and ADAM17
    Authors: PC Buchanan, KL Boylan, B Walcheck, R Heinze, MA Geller, PA Argenta, AP Skubitz
    J. Biol. Chem, 2017;0(0):. 2017
  8. Structural Determinants of the Gain-of-Function Phenotype of Human Leukemia-associated Mutant CBL Oncogene
    Authors: SA Nadeau, W An, BC Mohapatra, I Mushtaq, TA Bielecki, H Luan, N Zutshi, G Ahmad, MD Storck, M Sanada, S Ogawa, V Band, H Band
    J. Biol. Chem, 2017;292(9):3666-3682. 2017
  9. Antagonizing effects of membrane-acting androgens on the eicosanoid receptor OXER1 in prostate cancer
    Authors: K Kalyvianak, V Gebhart, N Peroulis, C Panagiotop, F Kiagiadaki, I Pediaditak, M Aivaliotis, E Moustou, M Tzardi, G Notas, E Castanas, M Kampa
    Sci Rep, 2017;7(0):44418. 2017
  10. Secreted Protein Acidic and Rich in Cysteine (SPARC) Enhances Cell Proliferation, Migration, and Epithelial Mesenchymal Transition, and SPARC Expression is Associated with Tumor Grade in Head and Neck Cancer.
    Authors: Chang C, Yen M, Liao S, Hsu Y, Lai C, Chang K, Hsu Y
    Int J Mol Sci, 2017;18(7):. 2017
  11. Galectin-3 interacts with the cell surface glycoprotein CD146 (MCAM, MUC18) and induces secretion of metastasis-promoting cytokines from vascular endothelial cells
    Authors: F Colomb, W Wang, D Simpson, M Zafar, R Beynon, JM Rhodes, LG Yu
    J. Biol. Chem., 2017;0(0):. 2017
  12. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017;8(7):e2955. 2017
  13. The HIV-1 viral synapse signals human foreskin keratinocytes to secrete thymic stromal lymphopoietin facilitating HIV-1 foreskin entry
    Authors: Z Zhou, L Xu, A Sennepin, C Federici, Y Ganor, D Tudor, D Damotte, N Barry Delo, M Zerbib, M Bomsel
    Mucosal Immunol, 2017;0(0):. 2017
  14. Isorhynchophylline, a Potent Plant Alkaloid, Induces Apoptotic and Anti-Metastatic Effects in Human Hepatocellular Carcinoma Cells through the Modulation of Diverse Cell Signaling Cascades
    Authors: H Lee, SH Baek, JH Lee, C Kim, JH Ko, SG Lee, A Chinnatham, SA Alharbi, WM Yang, JY Um, G Sethi, KS Ahn
    Int J Mol Sci, 2017;18(5):. 2017
  15. Disruption of Aneuploidy and Senescence induced by Aurora inhibition promotes intrinsic Apoptosis in double hit or double expressor Diffuse Large B-cell Lymphomas
    Authors: D Mahadevan, S Islam, W Qi, C Morales, L Cooke, C Spier, E Weterings
    Mol. Cancer Ther., 2017;0(0):. 2017
  16. JAK1/STAT3 activation through a proinflammatory cytokine pathway leads to resistance to molecularly targeted therapy in non-small cell lung cancer
    Authors: K Shien, VA Papadimitr, D Ruder, C Behrens, L Shen, N Kalhor, J Song, JJ Lee, J Wang, X Tang, RS Herbst, S Toyooka, L Girard, JD Minna, JM Kurie, II Wistuba, JG Izzo
    Mol. Cancer Ther., 2017;0(0):. 2017
  17. Secreted Protein Acidic and Rich in Cysteine (SPARC) Enhances Cell Proliferation, Migration, and Epithelial Mesenchymal Transition, and SPARC Expression is Associated with Tumor Grade in Head and Neck Cancer
    Authors: CH Chang, MC Yen, SH Liao, YL Hsu, CS Lai, KP Chang, YL Hsu
    Int J Mol Sci, 2017;18(7):. 2017
  18. Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells
    Authors: GM Pio, Y Xia, MM Piaseczny, JE Chu, AL Allan
    PLoS ONE, 2017;12(5):e0177640. 2017
  19. Co-targeting of MEK and PDGFR/STAT3 pathways to treat pancreatic ductal adenocar
    Authors: N Sahu, E Chan, F Chu, T Pham, H Koeppen, W Forrest, M Merchant, J Settleman
    Mol. Cancer Ther., 2017;0(0):. 2017
  20. Enhanced efficacy of AKT and FAK kinase combined inhibition in squamous cell lung carcinomas with stable reduction in PTEN
    Authors: A Cavazzoni, S La Monica, R Alfieri, A Ravelli, N Van Der St, R Sciarrillo, D Madeddu, CAM Lagrasta, F Quaini, M Bonelli, C Fumarola, D Cretella, G Digiacomo, M Tiseo, GJ Peters, A Ardizzoni, PG Petronini, E Giovannett
    Oncotarget, 2017;0(0):. 2017
  21. Plasma Extracellular Vesicles Enriched for Neuronal Origin: A Potential Window into Brain Pathologic Processes
    Authors: M Mustapic, E Eitan, JK Werner, ST Berkowitz, MP Lazaropoul, J Tran, EJ Goetzl, D Kapogianni
    Front Neurosci, 2017;11(0):278. 2017
  22. Retro-inverso Urokinase Receptor Antagonists for the Treatment of Metastatic Sarcomas
    Authors: MV Carriero, K Bifulco, V Ingangi, S Costantini, G Botti, C Ragone, M Minopoli, ML Motti, D Rea, G Scognamigl, G Botti, C Arra, G Ciliberto, A Pessi
    Sci Rep, 2017;7(1):1312. 2017
  23. Uric acid priming in human monocytes is driven by the AKT-PRAS40 autophagy pathway
    Authors: TO Cri?an, MCP Cleophas, B Novakovic, K Erler, FL van de Vee, HG Stunnenber, MG Netea, CA Dinarello, LAB Joosten
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017
  24. Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell
    Authors: S Chen, J Tao, F Zhong, Y Jiao, J Xu, Q Shen, H Wang, S Fan, Y Zhang
    PLoS ONE, 2017;12(5):e0176501. 2017
  25. Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
    Authors: Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R
    J Biol Chem, 2016;291(4):1652-63. 2016
  26. Characterization of non-olfactory GPCRs in human sperm with a focus on GPR18
    Sci Rep, 2016;6(0):32255. 2016
  27. Cisplatin-induced mesenchymal stromal cells-mediated mechanism contributing to decreased antitumor effect in breast cancer cells.
    Authors: Skolekova S, Matuskova M, Bohac M, Toro L, Demkova L, Gursky J, Kucerova L
    Cell Commun Signal, 2016;14(1):4. 2016
  28. CBP/p300 acetyltransferases regulate the expression of NKG2D ligands on tumor cells
    Oncogene, 2016;0(0):. 2016
  29. BRCA1-deficient breast cancer cell lines are resistant to MEK inhibitors and show distinct sensitivities to 6-thioguanine
    Authors: Yuexi Gu
    Sci Rep, 2016;6(0):28217. 2016
  30. Necrotic cells influence migration and invasion of glioblastoma via NF-?B/AP-1-mediated IL-8 regulation
    Authors: SH Ahn, H Park, YH Ahn, S Kim, MS Cho, JL Kang, YH Choi
    Sci Rep, 2016;6(0):24552. 2016
  31. Metabolism regulates cellular functions of bone marrow-derived cells used for cardiac therapy
    Stem Cells, 2016;0(0):. 2016
  32. Potential Function of Exogenous Vimentin on the Activation of Wnt Signaling Pathway in Cancer Cells
    J Cancer, 2016;7(13):1824-1832. 2016
  33. Neurotensin stimulates sortilin and mTOR in human microglia inhibitable by methoxyluteolin, a potential therapeutic target for autism
    Proc Natl Acad Sci USA, 2016;0(0):. 2016
  34. New insights into the Shwachman-Diamond Syndrome-related haematological disorder: hyper-activation of mTOR and STAT3 in leukocytes
    Sci Rep, 2016;6(0):33165. 2016
  35. Murine Polyomavirus Cell Surface Receptors Activate Distinct Signaling Pathways Required for Infection
    MBio, 2016;7(6):. 2016
  36. Capilliposide from Lysimachia capillipes inhibits AKT activation and restores gefitinib sensitivity in human non-small cell lung cancer cells with acquired gefitinib resistance
    Acta Pharmacol. Sin., 2016;0(0):. 2016
  37. Phosphorylation of calcium/calmodulin-stimulated protein kinase II at T286 enhances invasion and migration of human breast cancer cells
    Sci Rep, 2016;6(0):33132. 2016
  38. Long non-coding RNA urothelial carcinoma associated 1 (UCA1) mediates radiation response in prostate cancer
    Oncotarget, 2016;0(0):. 2016
  39. Hypoxia potentiates the cytotoxic effect of piperlongumine in pheochromocytoma models
    Oncotarget, 2016;7(26):40531-40545. 2016
  40. A Small Molecule Inhibitor of PDK1/PLC?1 Interaction Blocks Breast and Melanoma Cancer Cell Invasion
    Authors: C Raimondi, V Calleja, R Ferro, A Fantin, AM Riley, BV Potter, CH Brennan, T Maffucci, B Larijani, M Falasca
    Sci Rep, 2016;6(0):26142. 2016
  41. Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody.
    Authors: Mailly L, Xiao F, Lupberger J, Wilson G, Aubert P, Duong F, Calabrese D, Leboeuf C, Fofana I, Thumann C, Bandiera S, Lutgehetmann M, Volz T, Davis C, Harris H, Mee C, Girardi E, Chane-Woon-Ming B, Ericsson M, Fletcher N, Bartenschlager R, Pessaux P, Vercauteren K, Meuleman P, Villa P, Kaderali L, Pfeffer S, Heim M, Neunlist M, Zeisel M, Dandri M, McKeating J, Robinet E, Baumert T
    Nat Biotechnol, 2015;33(5):549-54. 2015
  42. Loss of DGKepsilon induces endothelial cell activation and death independently of complement activation.
    Authors: Bruneau S, Neel M, Roumenina L, Frimat M, Laurent L, Fremeaux-Bacchi V, Fakhouri F
    Blood, 2015;125(6):1038-46. 2015
  43. Combination of pan-RAF and MEK inhibitors in NRAS mutant melanoma.
    Authors: Atefi M, Titz B, Avramis E, Ng C, Wong D, Lassen A, Cerniglia M, Escuin-Ordinas H, Foulad D, Comin-Anduix B, Graeber T, Ribas A
    Mol Cancer, 2015;14(0):27. 2015
  44. A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D.
    Authors: Eggink L, Spyroulias G, Jones N, Hanson C, Hoober J
    PLoS ONE, 2015;10(6):e0130532. 2015
  45. Akt Kinase-Interacting Protein 1 Signals through CREB to Drive Diffuse Malignant Mesothelioma.
    Authors: Yamada T, Amann J, Fukuda K, Takeuchi S, Fujita N, Uehara H, Iwakiri S, Itoi K, Shilo K, Yano S, Carbone D
    Cancer Res, 2015;75(19):4188-97. 2015
  46. Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
    Authors: Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J
    Oncogene, 2015;34(43):5447-59. 2015
  47. Application of a Persistent Heparin Treatment Inhibits the Malignant Potential of Oral Squamous Carcinoma Cells Induced by Tumor Cell-Derived Exosomes.
    Authors: Sento S, Sasabe E, Yamamoto T
    PLoS ONE, 2015;11(2):e0148454. 2015
  48. YM155 potently kills acute lymphoblastic leukemia cells through activation of the DNA damage pathway.
    Authors: Chang B, Johnson K, LaTocha D, Rowley J, Bryant J, Burke R, Smith R, Loriaux M, Muschen M, Mullighan C, Druker B, Tyner J
    J Hematol Oncol, 2015;8(0):39. 2015
  49. Targeted inhibition of MEK1 by cobimetinib leads to differentiation and apoptosis in neuroblastoma cells.
    Authors: Singh A, Ruan Y, Tippett T, Narendran A
    J Exp Clin Cancer Res, 2015;34(0):104. 2015
  50. The Thrombin-Derived Host Defense Peptide GKY25 Inhibits Endotoxin-Induced Responses through Interactions with Lipopolysaccharide and Macrophages/Monocytes.
    Authors: Hansen F, Kalle-Brune M, van der Plas M, Stromdahl A, Malmsten M, Morgelin M, Schmidtchen A
    J Immunol, 2015;194(11):5397-406. 2015
  51. Exosomes released by chronic lymphocytic leukemia cells induce the transition of stromal cells into cancer-associated fibroblasts.
    Authors: Paggetti J, Haderk F, Seiffert M, Janji B, Distler U, Ammerlaan W, Kim Y, Adam J, Lichter P, Solary E, Berchem G, Moussay E
    Blood, 2015;126(9):1106-17. 2015
  52. Extracellular superoxide dismutase regulates the expression of small gtpase regulatory proteins GEFs, GAPs, and GDI.
    Authors: Laukkanen, Mikko O, Cammarota, Francesc, Esposito, Tiziana, Salvatore, Marco, Castellone, Maria D
    PLoS ONE, 2015;10(3):e0121441. 2015
  53. Hyperthermia Sensitizes Glioma Stem-like Cells to Radiation by Inhibiting AKT Signaling.
    Authors: Man J, Shoemake J, Ma T, Rizzo A, Godley A, Wu Q, Mohammadi A, Bao S, Rich J, Yu J
    Cancer Res, 2015;75(8):1760-9. 2015
  54. Specific activation of insulin-like growth factor-1 receptor by ginsenoside Rg5promotes angiogenesis and vasorelaxation.
    Authors: Cho Y, Hur S, Kim J, Kim J, Lee D, Choe J, Won M, Ha K, Jeoung D, Han S, Ryoo S, Lee H, Min J, Kwon Y, Kim D, Kim Y
    J Biol Chem, 2015;290(1):467-77. 2015
  55. A chromatin modifier genetic screen identifies SIRT2 as a modulator of response to targeted therapies through the regulation of MEK kinase activity.
    Authors: Bajpe, P K, Prahallad, A, Horlings, H, Nagtegaal, I, Beijersbergen, R, Bernards, R
    Oncogene, 2015;34(4):531-6. 2015
  56. Neurotrophin receptor TrkB promotes lung adenocarcinoma metastasis.
    Authors: Sinkevicius, Kerstin, Kriegel, Christin, Bellaria, Kelly J, Lee, Jaewon, Lau, Allison, Leeman, Kristen, Zhou, Pengchen, Beede, Alexande, Fillmore, Christin, Caswell, Deborah, Barrios, Juliana, Wong, Kwok-Kin, Sholl, Lynette, Schlaeger, Thorsten, Bronson, Roderick, Chirieac, Lucian R, Winslow, Monte M, Haigis, Marcia C, Kim, Carla F
    Proc Natl Acad Sci U S A, 2014;111(28):10299-304. 2014
  57. Sensitizing the therapeutic efficacy of taxol with shikonin in human breast cancer cells.
    Authors: Li, Wenjuan, Liu, Joan, Jackson, Kasey, Shi, Runhua, Zhao, Yunfeng
    PLoS ONE, 2014;9(4):e94079. 2014
  58. Overcoming acquired resistance to cetuximab by dual targeting HER family receptors with antibody-based therapy.
    Authors: Iida, Mari, Brand, Toni M, Starr, Megan M, Huppert, Evan J, Luthar, Neha, Bahrar, Harsh, Coan, John P, Pearson, Hannah E, Salgia, Ravi, Wheeler, Deric L
    Mol Cancer, 2014;13(0):242. 2014
  59. EGF potentiation of VEGF production is cell density dependent in H292 EGFR wild type NSCLC cell line.
    Authors: Ranayhossaini D, Lu J, Mabus J, Gervais A, Lingham R, Fursov N
    Int J Mol Sci, 2014;15(10):17686-704. 2014
  60. Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.
    Authors: Lutay N, Hakansson G, Alaridah N, Hallgren O, Westergren-Thorsson G, Godaly G
    PLoS ONE, 2014;9(1):e86466. 2014
  61. The epidermal growth factor receptor critically regulates endometrial function during early pregnancy.
    Authors: Large M, Wetendorf M, Lanz R, Hartig S, Creighton C, Mancini M, Kovanci E, Lee K, Threadgill D, Lydon J, Jeong J, DeMayo F
    , 2014;0(0):. 2014
  62. Enhancement of T cell responses as a result of synergy between lower doses of radiation and T cell stimulation.
    Authors: Spary L, Al-Taei S, Salimu J, Cook A, Ager A, Watson H, Clayton A, Staffurth J, Mason M, Tabi Z
    J Immunol, 2014;192(7):3101-10. 2014
  63. Extracellular UDP-glucose activates P2Y14 Receptor and Induces Signal Transducer and Activator of Transcription 3 (STAT3) Tyr705 phosphorylation and binding to hyaluronan synthase 2 (HAS2) promoter, stimulating hyaluronan synthesis of keratinocytes.
    Authors: Jokela T, Karna R, Makkonen K, Laitinen J, Tammi R, Tammi M
    J Biol Chem, 2014;289(26):18569-81. 2014
  64. Adenosine inhibits tumor cell invasion via receptor-independent mechanisms.
    Authors: Virtanen S, Kukkonen-Macchi A, Vainio M, Elima K, Harkonen P, Jalkanen S, Yegutkin G
    Mol Cancer Res, 2014;12(12):1863-74. 2014
  65. Targeting cadherin-17 inactivates Ras/Raf/MEK/ERK signaling and inhibits cell proliferation in gastric cancer.
    Authors: Lin Z, Zhang C, Zhang M, Xu D, Fang Y, Zhou Z, Chen X, Qin N, Zhang X
    PLoS ONE, 2014;9(1):e85296. 2014
  66. Histamine contributes to tissue remodeling via periostin expression.
    Authors: Yang, Lingli, Murota, Hiroyuki, Serada, Satoshi, Fujimoto, Minoru, Kudo, Akira, Naka, Tetsuji, Katayama, Ichiro
    J Invest Dermatol, 2014;134(8):2105-13. 2014
  67. Heparin-binding epidermal growth factor-like growth factor eliminates constraints on activated Kras to promote rapid onset of pancreatic neoplasia.
    Authors: Ray, K C, Moss, M E, Franklin, J L, Weaver, C J, Higginbotham, J, Song, Y, Revetta, F L, Blaine, S A, Bridges, L R, Guess, K E, Coffey, R J, Crawford, H C, Washington, M K, Means, A L
    Oncogene, 2014;33(7):823-31. 2014
  68. Elevated protein kinase D3 (PKD3) expression supports proliferation of triple-negative breast cancer cells and contributes to mTORC1-S6K1 pathway activation.
    Authors: Huck, Bettina, Duss, Stephan, Hausser, Angelika, Olayioye, Monilola
    J Biol Chem, 2014;289(6):3138-47. 2014
  69. Soft matrix is a natural stimulator for cellular invasiveness.
    Authors: Gu, Zhizhan, Liu, Fei, Tonkova, Elina A, Lee, Soo Youn, Tschumperlin, Daniel J, Brenner, Michael
    Mol Biol Cell, 2014;25(4):457-69. 2014
  70. Insulin-like-growth-factor-binding-protein-3 (IGFBP-3) contrasts melanoma progression in vitro and in vivo.
    Authors: Naspi A, Panasiti V, Abbate F, Roberti V, Devirgiliis V, Curzio M, Borghi M, Lozupone F, Carotti S, Morini S, Gaudio E, Calvieri S, Londei P
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  71. Downstream signaling and genome-wide regulatory effects of PTK7 pseudokinase and its proteolytic fragments in cancer cells.
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    Cell Commun Signal, 2014;12(0):15. 2014
  72. The molecular mechanism underlying the proliferating and preconditioning effect of vitamin C on adipose-derived stem cells.
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    Stem Cells Dev, 2014;23(12):1364-76. 2014
  73. Insights in dynamic kinome reprogramming as a consequence of MEK inhibition in MLL-rearranged AML.
    Authors: Kampen, K R, Ter Elst, A, Mahmud, H, Scherpen, F J G, Diks, S H, Peppelenbosch, M P, de Haas, V, Guryev, V, de Bont, E S J M
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    EMBO J., 2012;31(17):3513-23. 2012
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  104. Proteome profiling reveals potential toxicity and detoxification pathways following exposure of BEAS-2B cells to engineered nanoparticle titanium dioxide.
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    Proteomics, 2011;11(12):2406-22. 2011
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    Cancer Gene Ther., 2011;18(7):520-30. 2011
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  108. Influence of leucine on protein metabolism, phosphokinase expression, and cell proliferation in human duodenum1,3.
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Average Rating: 4.5 (Based on 8 reviews)

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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
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Excellent
  Anonymous 10/20/2017
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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
: Proteome Profiler Human Phospho-Kinase Array Kit [ARY003B]
 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
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  Anonymous 02/13/2017
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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
: Proteome Profiler Human Phospho-Kinase Array Kit [ARY003B]

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Other Experimental DetailsGreat kit and ok value for money considering the amount of data you get!
 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
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Excellent
  Anonymous 02/12/2017
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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
: Proteome Profiler Human Phospho-Kinase Array Kit [ARY003B]

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Other Experimental DetailsUsed with human samples. Image shown was at 1 minute. Other 'dots' appeared at increased exposure. Kit very good overall. Good quality result and easy to use
  Excellent
  Anonymous 01/03/2017
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Other Experimental Details

Other Experimental DetailsThis array is an excellent tool for screening potential phospho-kinases and related targets. However, it would be nice that if the total level of non-phosphorylated proteins be visualized at the same time on the membrane. In this case, the phosphorylated signals can be normalized.
 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
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Average
  Anonymous 10/28/2016
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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
: Proteome Profiler Human Phospho-Kinase Array Kit [ARY003B]

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Other Experimental DetailsAntibodies could not be sourced to validate some of the targets.
 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
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Excellent
  Anonymous 10/14/2016
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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
: Proteome Profiler Human Phospho-Kinase Array Kit [ARY003B]
 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
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Excellent
  Anonymous 08/09/2016
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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
: Proteome Profiler Human Phospho-Kinase Array Kit [ARY003B]
 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
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Very Good
  Anonymous 10/26/2015
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 Proteome Profiler Human Phospho-Kinase Array Kit ARY003B
: Proteome Profiler Human Phospho-Kinase Array Kit [ARY003B]

Summary

Sample TestedCell Lysates

Other Experimental Details

Other Experimental DetailsThis was control and didnt show any non specific bindings.
Specificity: Specific
Sensitivity: Sensitive
Buffer: BSA
Dilution: 1:1000

FAQs

  1. Does Lysis Buffer 6 in the Proteome Profiler™ Array contain phosphatase and protease inhibitors?

    • Lysis Buffer 6 contains phosphatase inhibitors but not protease inhibitors. If desired, protease inhibitors can be added to the lysis buffer immediately prior to use. We recommend 10 μg/ml Aprotinin (Tocris; Catalog # 4139), 10 μg/ml Leupeptin (Tocris; Catalog # 1167), and 10 μg/ml Peptstatin (Tocris; Catalog # 1190).

  2. Does Lysis Buffer 6 in the Proteome Profiler™ Array contain phosphatase and protease inhibitors?

    • Lysis Buffer 6 contains phosphatase inhibitors but not protease inhibitors. If desired, protease inhibitors can be added to the lysis buffer immediately prior to use. We recommend 10 μg/ml Aprotinin (Tocris; Catalog # 4139), 10 μg/ml Leupeptin (Tocris; Catalog # 1167), and 10 μg/ml Peptstatin (Tocris; Catalog # 1190).

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