Proteome Profiler Human XL Cytokine Array Kit

Newer Version Available: ARY022B
Contains 4 membranes - each spotted in duplicate with 102 different cytokine antibodies

Discontinued Product

ARY022 has been discontinued and is replaced by ARY022B.

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Citations (39)
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Proteome Profiler Human XL Cytokine Array Kit Summary

Proteome Profiler™ Human XL Cytokine Array Kit

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines.

 

Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Key Benefits

  • Detects 102 human cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

Principle of the Array

The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

Kit Contents

  • Rectangular 4-Well Multi-dish
  • 4 Human XL Cytokine Array nitrocellulose membranes spotted with 102 different antibodies to human cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
Adiponectin/Acrp30 IFN-gamma Lipocalin-2/NGAL
Aggrecan IGFBP-2 CCL2/MCP-1
Angiogenin IGFBP-3 CCL7/MCP-3
Angiopoietin-1 IL-1 alpha/IL-1F1 M-CSF
Angiopoietin-2 IL-1 beta/IL-1F2 MIF
BAFF/BLyS/TNFSF13B IL-1ra/IL-1F3 CXCL9/MIG
BDNF IL-2 CCL3/CCL4 MIP-1 alpha/beta
CD14 IL-3 CCL20/MIP-3 alpha
CD30 IL-4 CCL19/MIP-3 beta
CD40 Ligand/TNFSF5 IL-5 MMP-9
Chitinase 3-like IL-6 Myeloperoxidase
Complement Component C5/C5a IL-8 Osteopontin (OPN)
Complement Factor D IL-10 PDGF-AA
C-Reactive Protein/CRP IL-11 PDGF-AB/BB
Cripto-1 IL-12 p70 Pentraxin 3/TSF-14
Cystatin C IL-13 CXCL4/PF4
Dkk-1 IL-15 RAGE
DPPIV/CD26 IL-16 CCL5/RANTES
EGF IL-17A RBP4
CXCL5/ENA-78 IL-18 BPa Relaxin-2
Endoglin/CD105 IL-19 Resistin
EMMPRIN IL-22 CXCL12/SDF-1 alpha
Fas Ligand IL-23 Serpin E1/PAI-1
FGF basic IL-24 SHBG
KGF/FGF-7 IL-27 ST2/IL1 R4
FGF-19 IL-31 CCL17/TARC
Flt-3 Ligand IL-32 alpha/beta/gamma TFF3
G-CSF IL-33 TfR
GDF-15 IL-34 TGF-alpha
GM-CSF CXCL10/IP-10 Thrombospondin-1
CXCL1/GRO alpha CXCL11/I-TAC TNF-alpha
Growth Hormone (GH) Kallikrein 3/PSA uPAR
HGF Leptin VEGF
ICAM-1/CD54 LIF Vitamin D BP

 

Stability and Storage

Store the unopened kit at 2°C to 8°C. Do not use past kit expiration date.

Data Examples

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

Figure 1A.
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Figure 1A. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours.

Figure 1B
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Figure 1B. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours.

Figure 1C
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Figure 1C. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Proteome Profiler Human XL Cytokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis
    Authors: W Liu, P Wang, Z Xie, S Wang, M Ma, J Li, M Li, S Cen, S Tang, G Zheng, G Ye, X Wu, Y Wu, H Shen
    Cell Death Dis, 2019;10(3):188.  2019
  2. The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method
    Authors: D Kardos, M Simon, G Vácz, A Hinsenkamp, T Holczer, D Cseh, A Sárközi, K Szenthe, F Bánáti, S Szathmary, S Nehrer, O Kuten, M Masteling, Z Lacza, I Hornyák
    Int J Mol Sci, 2019;20(3):.  2019
  3. Gle1 mediates stress granule-dependent survival during chemotoxic stress
    Authors: L Glass, SR Wente
    Adv Biol Regul, 2018;0(0):.  2018
  4. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
    Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
    Nature, 2018;0(0):.  2018
  5. Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
    Authors: JM Cha, EK Shin, JH Sung, GJ Moon, EH Kim, YH Cho, HD Park, H Bae, J Kim, OY Bang
    Sci Rep, 2018;8(1):1171.  2018
  6. Unique and shared inflammatory profiles of human brain endothelia and pericytes
    Authors: LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow
    J Neuroinflammation, 2018;15(1):138.  2018
  7. USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and ?-TrCP and promotes cancer malignancy
    Authors: YC Wang, YS Wu, CY Hung, SA Wang, MJ Young, TI Hsu, JJ Hung
    Nat Commun, 2018;9(1):3996.  2018
  8. ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation
    Authors: L Walenta, D Fleck, T Fröhlich, H von Eysmon, GJ Arnold, J Spehr, JU Schwarzer, FM Köhn, M Spehr, A Mayerhofer
    Sci Rep, 2018;8(1):1431.  2018
  9. Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines
    Authors: M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano
    Int J Med Sci, 2018;15(12):1365-1372.  2018
  10. Ca2+ Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
    Authors: K Eubler, C Herrmann, A Tiefenbach, FM Köhn, JU Schwarzer, L Kunz, A Mayerhofer
    Int J Mol Sci, 2018;19(9):.  2018
  11. Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts
    Authors: T Kifune, H Ito, M Ishiyama, S Iwasa, H Takei, T Hasegawa, M Asano, T Shirakawa
    J Oral Sci, 2018;60(4):519-525.  2018
  12. Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro
    Authors: MD Wood, J Mukherjee, RO Pieper
    Sci Rep, 2018;8(1):5805.  2018
  13. Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
    Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
    Cell Death Dis, 2018;9(2):154.  2018
  14. Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
    Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
    Sci Adv, 2018;4(2):e1701729.  2018
  15. Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
    Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
    Cell Rep, 2018;24(9):2300-2311.  2018
  16. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018;9(1):3267.  2018
  17. Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
    Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
    Front Physiol, 2018;9(0):1025.  2018
  18. A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
    Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
    J Oral Sci, 2018;0(0):.  2018
  19. Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
    Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
    Cancer Med, 2018;0(0):.  2018
  20. Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
    Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
    J. Cell. Mol. Med., 2018;0(0):.  2018
  21. A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
    Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
    Cell Rep, 2018;25(13):3706-3720.e8.  2018
  22. Genetic and pharmacological validation of TAK1 inhibition in macrophages as a therapeutic strategy to effectively inhibit TNF secretion
    Authors: SA Scarneo, A Mansourati, LS Eibschutz, J Totzke, JR Roques, D Loiselle, D Carlson, P Hughes, TAJ Haystead
    Sci Rep, 2018;8(1):17058.  2018
  23. Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
    Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
    Cell Death Dis, 2018;9(5):506.  2018
  24. Crosstalk between stromal components and tumor cells of TNBC via secreted factors enhances tumor growth and metastasis
    Authors: K Jin, NB Pandey, AS Popel
    Oncotarget, 2017;0(0):.  2017
  25. Histone Deacetylase 1 Depletion Activates Human Cardiac Mesenchymal Stromal Cell Proangiogenic Paracrine Signaling Through a Mechanism Requiring Enhanced Basic Fibroblast Growth Factor Synthesis and Secretion
    Authors: JB Moore, J Zhao, AG Fischer, MCL Keith, D Hagan, M Wysoczynsk, R Bolli
    J Am Heart Assoc, 2017;6(7):.  2017
  26. An autocrine/paracrine circuit of growth differentiation factor (GDF) 15 has a role for maintenance of breast cancer stem-like cells
    Authors: A Sasahara, K Tominaga, T Nishimura, M Yano, E Kiyokawa, M Noguchi, M Noguchi, H Kanauchi, T Ogawa, H Minato, K Tada, Y Seto, A Tojo, N Gotoh
    Oncotarget, 2017;8(15):24869-24881.  2017
  27. Obesity Enhances the Conversion of Adipose-Derived Stromal/Stem Cells into Carcinoma-Associated Fibroblast Leading to Cancer Cell Proliferation and Progression to an Invasive Phenotype
    Authors: AL Strong, DT Pei, CG Hurst, JM Gimble, ME Burow, BA Bunnell
    Stem Cells Int, 2017;2017(0):9216502.  2017
  28. Lack of Evidence for Vasoactive and Inflammatory Mediators in the Promotion of Macular Edema Associated with Epiretinal Membranes
    Authors: BP Applewhite, S Babapoor-F, D Poon, SJ Hassan, E Wellmann, HS Ying, GL Semenza, S Montaner, A Sodhi
    Sci Rep, 2017;7(1):10608.  2017
  29. Protective Effect of Let-7 miRNA Family in Regulating Inflammation in Diabetes-Associated Atherosclerosis
    Authors: E Brennan, B Wang, A McClelland, M Mohan, M Marai, O Beuscart, S Derouiche, S Gray, R Pickering, C Tikellis, M de Gaetano, M Barry, O Belton, ST Ali-Shah, P Guiry, KA Jandeleit-, ME Cooper, C Godson, P Kantharidi
    Diabetes, 2017;0(0):.  2017
  30. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017;6(5):.  2017
  31. Extravillous Trophoblast and Endothelial Cell Crosstalk Mediates Leukocyte Infiltration to the Early Remodeling Decidual Spiral Arteriole Wall
    Authors: RH Choudhury, CE Dunk, SJ Lye, JD Aplin, LK Harris, RL Jones
    J. Immunol., 2017;0(0):.  2017
  32. Identification of Pathways in Liver Repair Potentially Targeted by Secretory Proteins from Human Mesenchymal Stem Cells
    Int J Mol Sci, 2016;17(7):.  2016
  33. Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer
    Nature, 2016;533(7604):493-8.  2016
  34. Diesel Exhaust Particles Upregulate Interleukins IL-6 and IL-8 in Nasal Fibroblasts
    Authors: Jin Ah Kim
    PLoS ONE, 2016;11(6):e0157058.  2016
  35. Transcriptional upregulation of c-MET is associated with invasion and tumor budding in colorectal cancer
    Oncotarget, 2016;0(0):.  2016
  36. Pretreatment of IL-1? enhances proliferation and chondrogenic potential of synovium-derived mesenchymal stem cells
    Authors: Etsuko Matsumura
    Cytotherapy, 2016;0(0):.  2016
  37. Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages
    Sci Rep, 2016;6(0):29588.  2016
  38. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
    Authors: Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H
    PLoS Pathog, 2015;11(12):e1005319.  2015
  39. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.  0

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