The verification of proper T cell function is crucial for any adoptive T cell transfer experiment. However, it is not currently clear which T cell characteristics are the most important for anti-tumor efficacy. It will be important to identify these characteristics and determine how to promote them to achieve more effective adoptive cell transfer strategies. We offer the reagents, kits, and assays needed to assess the characteristics of proper T cell function, including cytokine production, proliferation, and cytotoxicity.
Measure Cytokine Production
Proteome Profiler™ Antibody Arrays
Analyze the expression levels of up to 111 cytokines simultaneously, using only standard Western blotting equipment. Save time and discover novel responses with this unbiased approach.
|Cytokine Profile of TNF-alpha-treated Balb/3T3 Cells. Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of Recombinant Mouse TNF-alpha (Catalog # 410-MT) for 24 hours. Cell supernates were analyzed using the Mouse XL Cytokine Array Kit (Catalog # ARY028).
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Luminex® Assays and High Performance Assays
Build your own panel of up to 100 analytes with our Luminex Assays and follow up with our High Performance Assays that are benchmarked to our industry-leading Quantikine® ELISA kits.
ELISpot Kits and Development Modules
Identify and measure the frequency of cytokine secreting cells with the most accurate and sensitive ELISpot kits available. Our single and dual-color complete ELISpot kits are ready-to-run out of the box and require no assay development. Alternatively, we offer ELISpot Development Modules that contain the basic components required to develop your own ELISpot assay.
||Identification of IFNγ and IL-17 Secreting Cells. IFN-gamma (blue spots) and IL-17 (red spots) were secreted from mouse splenocytes stimulated with PMA/Ca2+ ionomycin. Spots of cytokine secretion were visualized using the Mouse IFNγ/IL-17 Dual-Color ELISpot Kit (Catalog ELD5007).
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ELISA Kits and Development Systems
Accurately and reliably measure IFN-gamma, TNF-alpha, and IL-2 levels with our ELISAs. Quantikine® ELISA kits are the most referenced ELISAs on the market and everything you need is included in the kit. If you want a more flexible option, we also offer DuoSet® ELISA Development Systems that are adaptable to multiple platforms. They include a capture and detection antibody, mass-calibrated standard, streptavidin-HRP, and a detailed protocol.
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Simple Plex™ Assays
Ella™ runs Simple Plex™ Assays. These novel assays are fully automated, quantitative, multianalyte immunoassays that will transform your research possibilities.
The Advantage of Simple Plex™ Assays
- Quantify up to 4 analytes in a single 25 µL sample. Ella™ is sensitive enough to detect sub-picogram levels of protein and has a 4-5 log dynamic range to help avoid sample dilution.
- Setup only takes 5 minutes. You simply pipette your samples into our cartridge, which automates the entire assay for you. Data is ready in 60 minutes.
- Over 120 human analytes are currently available. And with our catalog being powered by R&D Systems, our menu is unlimited. Just let us know what you need!
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Milo™ lets you identify cellular subpopulations that aren't visible in traditional Western Blots, so you can study distinct cell types in your heterogeneous sample. Milo™ gives you information about your cell populations that you can’t get any other way! Here’s how:
- Measure protein expression from approximately 1,000 individual cells using conventional Western blot antibodies.
- Get results in about four hours and measure up to four targets per cell simultaneously.
- Identify subpopulations of cells that can’t be measured with a conventional Western blot.
- Determine the efficiency of genetic engineering methods and analyze protein expression in genetically altered cells within low efficiency samples.
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TACS® XTT Cell Proliferation Assay
This proliferation assay utilizes XTT, which can be used to assay cell proliferation, cell viability, and/or cytotoxicity. The procedure avoids radioactivity and gives reproducible and sensitive results. XTT is added directly to the culture medium and cleavage of the tetrazolium salt to formazan occurs only in metabolically active cells.
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Calcein AM Cell Viability Kit
Calcein AM provides a simple, rapid, and accurate method to measure cell viability and/or cytotoxicity. Calcein AM is a non-fluorescent, hydrophobic compound that easily permeates intact, live cells. The hydrolysis of Calcein AM by intracellular esterases produces Calcein, a hydrophilic, strongly fluorescent compound that is well-retained in the cell cytoplasm. Cells grown in black-walled plates (Catalog # DY991) can be quantified in less than two hours using a fluorescence plate reader with excitation at 490 nm and emission at 520 nm. The kit contains reagents for ~1000 tests.
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