Proteome Profiler Mouse XL Cytokine Array

Contains 4 membranes-each spotted in duplicate with 111 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY028
Detection of selected mouse cytokines in Balb/3T3 Cell Line Lysate
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Product Details
Procedure
Citations (54)
FAQs
Reviews (22)

Proteome Profiler Mouse XL Cytokine Array Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and urine.

Troubleshooting Guide

Key Benefits

  • Detects 111 mouse cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 
 

 

Principle of the Assay

The Proteome Profiler Mouse XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

 
Simultaneously detect the levels of these cytokines, chemokines, and growth factors in a single sample.
Adiponectin/Acrp30 DPPIV/CD26 IL-27
Amphiregulin EGF IL-28
Angiopoietin-1 Endoglin/CD105 IL-33
Angiopoietin-2 Endostatin LDL R
Angiopoietin-like 3 Fetuin A/AHSG Leptin
BAFF/BLyS/TNFSF13B FGF acidic LIF
C1q R1/CD93 FGF-21 Lipocalin-2/NGAL,
CCL2/JE/MCP-1 Flt-3 Ligand LIX
CCL3/CCL4 MIP-1 alpha/beta Gas6 M-CSF
CCL5/RANTES G-CSF MMP-2
CCL6/C10 GDF-15 MMP-3
CCL11/Eotaxin GM-CSF MMP-9
CCL12/MCP-5 HGF Myeloperoxidase
CCL17/TARC ICAM-1/CD54 Osteopontin (OPN)
CCL19/MIP-3 beta IFN-gamma Osteoprotegerin/TNFRSF11B
CCL20/MIP-3 alpha IGFBP-1 PD-ECGF/Thymidine phosphorylase
CCL21/6Ckine IGFBP-2 PDGF-BB
CCL22/MDC IGFBP-3 Pentraxin 2/SAP
CD14 IGFBP-5 Pentraxin 3/ TSG-14
CD40/TNFRSF5 IGFBP-6 Periostin/OSF-2
CD160 IL-1 alpha/IL1F1 Pref-1/DLK-1/FA1
Chemerin IL-1 beta/IL-1F2 Proliferin
Chitinase 3-like 1 IL-1ra/IL-1F3 Proprotein Convertase 9/PCSK9
Coagulation Factor III/Tissue Factor IL-2 RAGE
Complement Component C5/C5a IL-3 RBP4
Complement Factor D IL-4 Reg3G
C-Reactive Protein/CRP IL-5 Resistin
CX3CL1/Fractalkine IL-6 E-Selectin/CD62E
CXCL1/KC IL-7 P-Selectin/CD62P
CXCL2/MIP-2 IL-10 Serpin E1/PAI-1
CXCL9/MIG IL-11 Serpin F1/PEDF
CXCL10/IP-10 IL-12p40 Thrombopoietin
CXCL11/I-TAC IL-13 TIM-1/KIM-1/HAVCR
CXCL13/BLC/BCA-1 IL-15 TNF-alpha
CXCL16 IL-17A VCAM-1/CD106
Cystatin C  IL-22 VEGF
Dkk-1 IL-23 WISP-1/CCN4

Assays for Analytes represented in the Mouse XL Cytokine Array Kit

Analyte Qkit Duoset
Adiponectin/Acrp30 MRP300   DY1119  
Amphiregulin     DY989  
Angiopoietin-1        
Angiopoietin-2        
Angiopoietin-like 3 MANL30   DY136  
BAFF/BLyS/TNFSF13B MBLYS0   DY2106-05  
C1q R1/CD93 MCD930      
CCL2/JE/MCP-1 MJE00   DY479  
CCL3/CCL4 MIP-1 alpha/beta        
CCL5/RANTES MMR00   DY478  
CCL6/C10     DY487  
CCL11/Eotaxin MME00   DY420  
CCL12/MCP-5 MCC120   DY428  
CCL17/TARC MCC170   DY529  
CCL19/MIP-3 beta     DY440  
CCL20/MIP-3 alpha MCC200   DY760  
CCL21/6Ckine     DY457  
CCL22/MDC MCC220   DY439  
CD14 MC140   DY982  
CD40/TNFRSF5 MCCD40   DY097  
CD160 MCHM00   DY2325  
Chemerin        
Chitinase 3-like 1 MC3L10   DY2649  
Coagulation Factor III/Tissue Factor        
Complement Component C5/C5a        
Complement Factor D        
C-Reactive Protein/CRP MCRP00   DY1829  
CX3CL1/Fractalkine MCX310   DY472  
CXCL1/KC MKC00B   DY453  
CXCL2/MIP-2 MM200   DY452  
CXCL9/MIG MCX900   DY492  
CXCL10/IP-10 MCX100   DY466  
CXCL11/I-TAC     DY572  
CXCL13/BLC/BCA-1 MCX130      
CXCL16     DY503  
Cystatin C  MSCTC0   DY1238  
Dkk-1 MKK100   DY1765  
DPPIV/CD26     DY954  
EGF MEG00   DY2028  
Endoglin/CD105 MNDG00   DY1320  
Endostatin        
Fetuin A/AHSG MFTA00   DY1563  
FGF acidic     DY4686-05  
FGF-21 MF2100   DY3057  
Flt-3 Ligand MFK00   DY427  
Gas6 MGAS60   DY986  
G-CSF MCS00   DY414  
GDF-15 MGD150   DY6385  
GM-CSF MGM00   DY415  
HGF MHG00   DY2207  
ICAM-1/CD54 MIC100   DY796  
IFN-gamma MIF00   DY485  
IGFBP-1     DY1588-05  
IGFBP-2     DY797  
IGFBP-3 MGB300   DY775  
IGFBP-5     DY578  
IGFBP-6     DY776  
IL-1 alpha/IL1F1 MLA00   DY400  
IL-1 beta/IL-1F2 MLB00C   DY401  
IL-1ra/IL-1F3 MRA00   DY480  
IL-2 M2000   DY402  
IL-3 M3000   DY403  
IL-4 M4000B   DY404  
IL-5 M5000   DY405  
IL-6 M6000B   DY406  
IL-7 M7000   DY407  
IL-10 M1000B   DY417  
IL-11     DY418  
IL-12p40 MP400
non-allele specific
M1240
allele specific
DY2398
non-allele specific
DY499
allele specific
IL-13 M1300CB   DY413  
IL-15     DY447  
IL-17A M1700   DY421  
IL-22 M2200   DY582  
IL-23 M2300   DY1887  
IL-27     DY1789B  
IL-28        
IL-33 M3300   DY3626  
LDL R MLDLR0   DY2255  
Leptin MOB00   DY498  
LIF MLF00      
Lipocalin-2/NGAL,     DY1857  
LIX MX000   DY443  
M-CSF MMC00   DY416  
MMP-2 MMP200      
MMP-3 MMP300   DY548  
MMP-9 MMPT90 MMP900B DY6718 DY909
Myeloperoxidase     DY3667  
Osteopontin (OPN) MOST00   DY441  
Osteoprotegerin/TNFRSF11B MOP00   DY459  
PD-ECGF/Thymidine phosphorylase        
PDGF-BB MBB00      
Pentraxin 2/SAP MPTX20      
Pentraxin 3/ TSG-14 MPTX30   DY2166  
Periostin/OSF-2 MOSF20   DY2955  
Pref-1/DLK-1/FA1        
Proliferin        
Proprotein Convertase 9/PCSK9 MPC900   DY3985  
RAGE MRG00   DY1179  
RBP4 MRBP40   DY3476  
Reg3G        
Resistin MRSN00   DY1069  
E-Selectin/CD62E MES00   DY575  
P-Selectin/CD62P MPS00   DY737  
Serpin E1/PAI-1     DY3828-05  
Serpin F1/PEDF        
Thrombopoietin MTP00   DY488  
TIM-1/KIM-1/HAVCR MKM100   DY1817  
TNF-alpha MTA00B   DY410  
VCAM-1/CD106 MVC00   DY643  
VEGF MMV00   DY493  
WISP-1/CCN4 MWSP10      

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

Product Datasheets

Data Example

Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Lysate. Lysates from Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (200 µg lysate, 10 minute exposure).

Detection of Selected Mouse Cytokines in CMT-93 Cell Line Lysate. Lysates from CMT-93 mouse rectal carcinoma cells were untreated or treated with 100 ng/mL LPS for 24 hours (200 µg lysate, 10 minute exposure).

Detection of Selected Mouse Cytokines in Balb/3T3 Cell Line Supernate. Balb/3T3 mouse embryonic fibroblast cells were untreated or treated with 100 ng/mL of recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) for 24 hours (500 µL of cell culture supernate, 5 minute exposure).

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, relative expression levels of mouse cytokines and chemokines in samples can be determined using the following procedure:

  • Prepare membrane and incubate with prepared sample
  • Incubate the membrane with Detection Antibody Cocktail
  • Incubate the membrane array with Streptavidin-HRP
  • Develop the membrane array with Chemi Reagents 1 and 2
  • Expose the membrane array to autoradiography film
 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse XL Cytokine Array nitrocellulose membranes spotted with 111 different antibodies to mouse cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

 

Other Supplies Required

Reagents

Materials

  • Pipettes and pipette tips
  • Gloves
  • Plastic container with the capacity to hold 50 mL (for washing the arrays)
  • Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
  • Plastic wrap
  • Absorbent lab wipes (KimWipes® or equivalent)
  • Paper towels
  • X-ray film (Kodak BioMax™ Light-1) or equivalent
  • Flat-tipped tweezers

Equipment

  • Rocking platform shaker
  • Microcentrifuge
  • Autoradiography cassette
  • Film developer
  • Flatbed scanner with transparency adapter capable of transmission mode
  • Computer capable of running image analysis software and Microsoft Excel

Other Supplies Required for Cell Lysate Samples

  • Phosphate-Buffered Saline (PBS)
  • Lysis buffer (1% Igepal CA-630, 20 mM Tris-HCl (pH 8.0), 137 mM NaCl, 10% glycerol, 2 mM EDTA, 10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)

Other Supplies Required for Tissue Lysate Samples

  • PBS with protease inhibitors (10 µg/mL Aprotinin, 10 µg/mL Leupeptin, and 10 µg/mL Pepstatin)
  • Triton X-100

 

Procedure Overview

R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler Mouse XL Cytokine Array Kit, Panel A (Catalog # ARY028)

Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

Place each array membrane in a separate well of the 4-Well Multi-dish.

Incubate for one hour on a rocking platform shaker.

Place each array membrane in a separate well of the 4-Well Multi-dish

Add 0.5 mL Array Buffer 4 to each sample.

Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.

Add 0.5 mL Array Buffer 4 to each sample.

Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.

Incubate overnight at 2 °C to 8 °C on a rocking platform.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

Add 0.5 mL Array Buffer 4 to each sample.
Add 30 µL of Detection Antibody Cocktail to 1.5 mL of Array Buffer 4/6 for each array.
Add 0.5 mL Array Buffer 4 to each sample.

Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.

Add 0.5 mL Array Buffer 4 to each sample.

Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.

Incubate for one hour on a rocking platform shaker.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

Add 0.5 mL Array Buffer 4 to each sample.

Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.

Place the array membrane in the diluted Streptavidin-HRP solution.

Incubate for 30 minutes on a rocking platform shaker.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Add 0.5 mL Array Buffer 4 to each sample.

Place the array membrane on a plastic sheet protector.

Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.

Cover the membrane with the top sheet of the plastic protector.

Incubate for 1 minute.

Blot off excess Chemi Reagent Mix.

Wrap the membrane and sheet protector in plastic wrap.

Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.

Add 0.5 mL Array Buffer 4 to each sample.

Citations for Proteome Profiler Mouse XL Cytokine Array

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

54 Citations: Showing 1 - 10
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  1. Preventing tumor progression to the bone by induced tumor-suppressing MSCs
    Authors: X Sun, K Li, R Zha, S Liu, Y Fan, D Wu, M Hase, UK Aryal, CC Lin, BY Li, H Yokota
    Theranostics, 2021;11(11):5143-5159.  2021
  2. Cell barrier function of resident peritoneal macrophages in post-operative adhesions
    Authors: T Ito, Y Shintani, L Fields, M Shiraishi, MN Podaru, S Kainuma, K Yamashita, K Kobayashi, M Perretti, F Lewis-McDo, K Suzuki
    Nature Communications, 2021;12(1):2232.  2021
  3. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression
    Authors: E Yeini, P Ofek, S Pozzi, N Albeck, D Ben-Shusha, G Tiram, S Golan, R Kleiner, R Sheinin, S Israeli Da, S Reich-Zeli, R Grossman, Z Ram, H Brem, TM Hyde, P Magod, D Friedmann-, A Madi, R Satchi-Fai
    Nature Communications, 2021;12(1):1912.  2021
  4. Integration of transcriptomics and system pharmacology to reveal the therapeutic mechanism underlying Qingfei Xiaoyan Wan to treat allergic asthma
    Authors: JY Hou, JR Wu, D Xu, YB Chen, DD Shang, S Liu, GW Fan, YL Cui
    Journal of ethnopharmacology, 2021;278(0):114302.  2021
  5. Microglia and macrophages promote corralling, wound compaction and recovery after spinal cord injury via Plexin-B2
    Authors: X Zhou, S Wahane, MS Friedl, M Kluge, CC Friedel, K Avrampou, V Zachariou, L Guo, B Zhang, X He, RH Friedel, H Zou
    Nat. Neurosci., 2020;23(3):337-350.  2020
  6. Sestrin2 modulates cardiac inflammatory response through maintaining redox homeostasis during ischemia and reperfusion
    Authors: D Ren, N Quan, J Fedorova, J Zhang, Z He, J Li
    Redox Biol, 2020;34(0):101556.  2020
  7. Adrenergic Blockade Promotes Maintenance of Dormancy in Prostate Cancer Through Upregulation of GAS6
    Authors: AM Decker, JT Decker, Y Jung, FC Cackowski, S Daignault-, TM Morgan, LD Shea, RS Taichman
    Transl Oncol, 2020;13(7):100781.  2020
  8. YAP Non-cell-autonomously Promotes Pluripotency Induction in Mouse Cells
    Authors: AA Hartman, SM Scalf, J Zhang, X Hu, X Chen, AE Eastman, C Yang, S Guo
    Stem Cell Reports, 2020;14(4):730-743.  2020
  9. High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cell differentiation
    Authors: W He, J Xu, R Mu, Q Li, DL Lv, Z Huang, J Zhang, C Wang, L Dong
    Nat Commun, 2020;11(1):1732.  2020
  10. SUMO E3 ligase PIAS1 is a potential biomarker indicating stress susceptibility
    Authors: HY Lin, YS Liu, CY Huang, F Cathomas, K Liu, J Wang, HT Cheng, SW Lai, YC Liu, CJ Chen, C Lin, DY Lu
    Psychoneuroendocrinology, 2020;120(0):104800.  2020
  11. Triterpenoids Extracted From Antrodia cinnamomea Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice via Suppression Inflammatory Response
    Authors: Y Liu, Z Wang, F Kong, L Teng, X Zheng, X Liu, D Wang
    Front Microbiol, 2020;11(0):1113.  2020
  12. The mechanism of Tyk2 deficiency-induced immunosuppression in mice involves robust IL-10 production in macrophages
    Authors: K Hirashima, R Muromoto, H Minoguchi, T Matsumoto, Y Kitai, JI Kashiwakur, K Shimoda, K Oritani, T Matsuda
    Cytokine, 2020;130(0):155077.  2020
  13. Cancer associated fibroblast FAK regulates malignant cell metabolism
    Authors: F Demirciogl, J Wang, J Candido, ASH Costa, P Casado, B de Luxan D, LE Reynolds, J Gomez-Escu, E Newport, V Rajeeve, AM Baker, M Roy-Luzarr, TA Graham, J Foster, Y Wang, JJ Campbell, R Singh, P Zhang, TJ Schall, FR Balkwill, J Sosabowski, PR Cutillas, C Frezza, P Sancho, K Hodivala-D
    Nat Commun, 2020;11(1):1290.  2020
  14. Evidence of mesenchymal stromal cell adaptation to local microenvironment following subcutaneous transplantation
    Authors: MB Preda, AM Lupan, CA Neculachi, LI Leti, IM Fenyo, S Popescu, EG Rusu, CI Marinescu, M Simionescu, A Burlacu
    J. Cell. Mol. Med., 2020;0(0):.  2020
  15. Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects
    Authors: CC Hu, CH Chang, YM Hsiao, Y Chang, YY Wu, SWN Ueng, MF Chen
    Int J Mol Sci, 2020;21(15):.  2020
  16. The Gustatory Sensory G-Protein GNAT3 Suppresses Pancreatic Cancer Progression in Mice
    Authors: MT Hoffman, SB Kemp, DJ Salas-Esca, Y Zhang, NG Steele, S The, D Long, S Benitz, W Yan, RF Margolskee, F Bednar, M Pasca di M, HJ Wen, HC Crawford
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.  2020
  17. Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity
    Authors: HL Mercer, LM Snyder, CM Doherty, BA Fox, DJ Bzik, EY Denkers
    PLoS Pathog., 2020;16(5):e1008572.  2020
  18. Yes-associated protein (YAP) induces a secretome phenotype and transcriptionally regulates plasminogen activator Inhibitor-1 (PAI-1) expression in hepatocarcinogenesis
    Authors: S Marquard, S Thomann, SME Weiler, M Bissinger, T Lutz, C Sticht, M Tóth, C de la Torr, N Gretz, BK Straub, J Marquardt, P Schirmache, K Breuhahn
    Cell Commun Signal, 2020;18(1):166.  2020
  19. Bisphenol A exposure increases epididymal susceptibility to infection in mice
    Authors: YJ Park, WK Pang, DY Ryu, EO Adegoke, MS Rahman, MG Pang
    Ecotoxicol Environ Saf, 2020;208(0):111476.  2020
  20. Adaptive thermogenesis enhances the life-threatening response to heat in mice with an Ryr1 mutation
    Authors: HJ Wang, CS Lee, RSZ Yee, L Groom, I Friedman, L Babcock, DK Georgiou, J Hong, AD Hanna, J Recio, JM Choi, T Chang, NH Agha, J Romero, P Sarkar, N Voermans, MW Gaber, SY Jung, ML Baker, RG Pautler, RT Dirksen, S Riazi, SL Hamilton
    Nat Commun, 2020;11(1):5099.  2020
  21. Dietary &alpha-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA Protection
    Authors: JS Russell, TA Griffith, S Naghipour, J Vider, EF Du Toit, HH Patel, JN Peart, JP Headrick
    Nutrients, 2020;12(9):.  2020
  22. Neuroblast senescence in the aged brain augments natural killer cell cytotoxicity leading to impaired neurogenesis and cognition
    Authors: WN Jin, K Shi, W He, JH Sun, L Van Kaer, FD Shi, Q Liu
    Nature Neuroscience, 2020;0(0):.  2020
  23. EGFRvIII uses intrinsic and extrinsic mechanisms to reduce glioma adhesion and increase migration
    Authors: A Banisadr, M Eick, P Beri, AD Parisian, B Yeoman, JK Placone, AJ Engler, F Furnari
    J Cell Sci, 2020;0(0):.  2020
  24. In situ neutrophil efferocytosis shapes T cell immunity to influenza infection
    Authors: K Lim, TH Kim, A Trzeciak, AM Amitrano, EC Reilly, H Prizant, DJ Fowell, DJ Topham, M Kim
    Nat. Immunol., 2020;0(0):.  2020
  25. Host cytosolic RNA sensing pathway promotes T Lymphocyte-mediated mycobacterial killing in macrophages
    Authors: Y Cheng, NJ Kiene, A Tatarian, EF Eix, JS Schorey
    PLoS Pathog., 2020;16(5):e1008569.  2020
  26. NOX4 Deficiency Exacerbates the Impairment of Cystatin C-Dependent Hippocampal Neurogenesis by a Chronic High Fat Diet
    Authors: P Jiranugrom, ID Yoo, MW Park, JH Ryu, JS Moon, SS Yi
    Genes (Basel), 2020;11(5):.  2020
  27. Dual microglia effects on blood brain barrier permeability induced by systemic inflammation
    Authors: K Haruwaka, A Ikegami, Y Tachibana, N Ohno, H Konishi, A Hashimoto, M Matsumoto, D Kato, R Ono, H Kiyama, AJ Moorhouse, J Nabekura, H Wake
    Nat Commun, 2019;10(1):5816.  2019
  28. Acidity promotes tumour progression by altering macrophage phenotype in prostate cancer
    Authors: A El-Kenawi, C Gatenbee, M Robertson-, R Bravo, J Dhillon, Y Balaguruna, A Berglund, N Visvakarma, A Ibrahim-Ha, J Choi, K Luddy, R Gatenby, S Pilon-Thom, A Anderson, B Ruffell, R Gillies
    Br. J. Cancer, 2019;121(7):556-566.  2019
  29. Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice
    Authors: F Hempel, M Roderfeld, R Savai, A Sydykov, K Irungbam, R Schermuly, R Voswinckel, K Köhler, Y Churin, L Kiss, J Bier, J Pons-Kühne, E Roeb
    Cells, 2019;8(10):.  2019
  30. Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway
    Authors: GS Hussey, JL Dziki, YC Lee, JG Bartolacci, M Behun, HR Turnquist, SF Badylak
    J Immunol Regen Med, 2019;3(0):26-35.  2019
  31. Non-classical tissue monocytes and two functionally distinct populations of interstitial macrophages populate the mouse lung
    Authors: J Schyns, Q Bai, C Ruscitti, C Radermecke, S De Scheppe, S Chakarov, F Farnir, D Pirottin, F Ginhoux, G Boeckxstae, F Bureau, T Marichal
    Nat Commun, 2019;10(1):3964.  2019
  32. Platelet GPIb? is a mediator and potential interventional target for NASH and subsequent liver cancer
    Authors: M Malehmir, D Pfister, S Gallage, M Szydlowska, D Inverso, E Kotsiliti, V Leone, M Peiseler, BGJ Surewaard, D Rath, A Ali, MJ Wolf, H Drescher, ME Healy, D Dauch, D Kroy, O Krenkel, M Kohlhepp, T Engleitner, A Olkus, T Sijmonsma, J Volz, C Deppermann, D Stegner, P Helbling, C Nombela-Ar, A Rafiei, M Hinterleit, M Rall, F Baku, O Borst, CL Wilson, J Leslie, T O'Connor, CJ Weston, DH Adams, L Sheriff, A Teijeiro, M Prinz, R Bogeska, N Anstee, MN Bongers, M Notohamipr, T Geisler, DJ Withers, J Ware, DA Mann, HG Augustin, A Vegiopoulo, MD Milsom, AJ Rose, PF Lalor, JM Llovet, R Pinyol, F Tacke, R Rad, M Matter, N Djouder, P Kubes, PA Knolle, K Unger, L Zender, B Nieswandt, M Gawaz, A Weber, M Heikenwald
    Nat. Med., 2019;25(4):641-655.  2019
  33. Prostate cancer promotes a vicious cycle of bone metastasis progression through inducing osteocytes to secrete GDF15 that stimulates prostate cancer growth and invasion
    Authors: W Wang, X Yang, J Dai, Y Lu, J Zhang, ET Keller
    Oncogene, 2019;0(0):.  2019
  34. Liver-derived fibroblast growth factor 21 mediates effects of glucagon-like peptide-1 in attenuating hepatic glucose output
    Authors: J Liu, K Yang, J Yang, W Xiao, Y Le, F Yu, L Gu, S Lang, Q Tian, T Jin, R Wei, T Hong
    EBioMedicine, 2019;0(0):.  2019
  35. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  2. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Mouse XL Cytokine Array

Average Rating: 4.9 (Based on 22 Reviews)

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Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 09/16/2021
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 08/10/2021
Application:

We used the array kit for profiling inflammatory cytokines in plasma samples from mice. I trust that it works very well.


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 03/14/2021
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 09/17/2020
Application:

Excellent antibody array to screen a multitude of cytokines, chemokines, and growth factors in one small strip of membrane. Readout is not a fluorescence so you don't need a special fluorescence reader. If you are doing Western blotting, you can do this easily.


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 02/12/2020
Application:

Mouse breast tumor tissues


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 01/25/2020
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 01/06/2020
Application:

Excellent array!!


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 06/06/2019
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 03/13/2019
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 12/05/2018
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 11/11/2018
Application:

Excellent specificity and detection for large number of cytokines. Works perfectly with little optimization.


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 11/05/2018
Application:

Pancreas lysate. 750ug protein/ membrane


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 07/18/2018
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 04/01/2018
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 03/22/2018
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 12/07/2017
Application:

200 uL pooled serum from wild-type C57Bl/6 mice.
5 min exposure time


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 08/17/2017
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 07/17/2017
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 05/23/2017
Application:

Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 11/28/2016
Application:

150 uL of mouse serum


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 09/30/2016
Application:

This is a great kit. It provides excellent reliability and a large number of cytokines, chemokines to screening in a single assay. I strongly recommend it.


Proteome Profiler Mouse XL Cytokine Array
By Anonymous on 08/26/2016
Application:

The sample was cell culture supernatant collected from MEF.