Luminex High Performance Assays

Luminex® High Performance Assays are our most accurate and precise bead-based multianalyte profiling kits. The assays rely on panel-optimized diluents that provide maximum performance for up to 22 analytes, depending on the panel selected. Analytes can be ordered either as a complete panel or as a smaller subset from any of the panels listed below. The kits can be used to measure target analytes in their validated sample types, which may include cell culture supernates, serum, plasma, urine, saliva, and milk.

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Features

  • All analytes in a given panel are optimized and tested together to ensure maximum performance for the entire panel
  • Kits undergo similar validation testing as the Quantikine® single analyte assays and are correlated with Quantikine ELISAs, when available
  • Kits consist of base kits and bead sets, which can be ordered individually or as premixed kits
  • Kits are available for human and rat analytes
  • All assays can be performed in 2.5-5 hrs
  • Assays require a small sample volume (<100 µL)
  • Kits containing beads that the user will mix are delivered 1 day after the order is placed; kits containing beads that we mix are delivered in 5-7 days
 

Luminex® High Performance Assays Correlate to Quantikine® ELISAs

Cell culture supernate from adipocytes were analyzed using both Luminex® High Performance Assays and Quantikine® ELISAs.   Cell culture supernate from SW1353 human chondrosarcoma cells were analyzed using both Luminex® High Performance Assays and Quantikine® ELISAs.
Cell culture supernate from adipocytes were analyzed using both Luminex® High Performance Assays and Quantikine® ELISAs. Cell culture supernate from pre-adipocytes before and after differentiation were harvested at the time points indicated and frozen.  Following collection, samples were analyzed using Human Leptin and Human Total MMP-3 Quantikine® ELISAs (blue bars; Catalog # DLP00 and DMP300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LUB000 and LMP000B, respectively).   Cell culture supernate from SW1353 human chondrosarcoma cells were analyzed using both Luminex® High Performance Assays and Quantikine® ELISAs. Cell culture supernate from SW1353 cells were either unstimulated or stimulated with 5 ng/mL IL-1 alpha and 10 ng/mL OSM, harvested 24 hours later, and frozen.  Following collection, samples were analyzed using Human CXCL10/IP-10, Human Total MMP-3, and Human Pro-MMP-13 Quantikine® ELISAs (blue bars; Catalog # DIP100, DMP300, and DM1300, respectively) and Luminex High Performance Human Cytokine Panel B and Human MMP Panel (orange bars; Catalog # LUB000 and LMP000B, respectively).

Kits are available in two formats: polystyrene or magnetic beads

Polystyrene beads: Polystyrene beads are designed for use with the Luminex 100, Luminex 200, or Bio-Rad® Bio-Plex® dual laser analyzers.

Magnetic beads: Magnetic beads are designed for use with the Luminex MAGPIX®, Luminex 100, Luminex 200, or Bio-Rad Bio-Plex analyzers.

To view a list of analytes available in the polystyrene or magnetic bead formats for the Luminex High Performance Assays, see the tables below.

Kit Contents*

Base Kits: Standard cocktail(s), bead diluent, biotin antibody diluent, calibrator diluent(s), Streptavidin-PE, wash buffer concentrate, mixing bottles, filter-bottom or round-bottom microplate, foil plate sealers

Bead Sets: Antibody-coated beads, biotinylated detection antibody cocktail
*Base Kits and Bead Sets can be ordered individually or as premixed kits.

 

Analytes available in the magnetic bead format

Human Angiogenesis Panel A

  • Angiogenin
  • Angiopoietin-1
  • Endostatin
  • FGF acidic
  • FGF basic
  • PDGF-AA
  • PDGF-BB
  • PlGF
  • Thrombospondin-2
  • VEGF
  • VEGF-D

Human Biomarker Panel A

  • BAFF/BLyS
  • CCL20/MIP-3 alpha
  • CD14
  • CD27/TNFRSF7
  • CXCL13/BLC/BCA-1
  • gp130
  • IL-2 R alpha
  • IL-6 R alpha
  • TNF RII/TNFRSF1B

Human Cardiac Panel A

  • CD40 Ligand/TNFSF5
  • GDF-15
  • Pappalysin-1/PAPP-A
  • PCSK9
  • ST2/IL-33 R
  • TNF RII/TNFRSF1B

Human Cardiac Panel B

  • C-Reactive Protein/CRP
  • Cystatin C
  • Myeloperoxidase/MPO
  • P-Selectin/CD62P
  • Serpin E1/PAI-1
  • TIMP-1

Human Cytokine Panel A

  • CCL2/MCP-1
  • CCL3/MIP-1 alpha
  • CCL4/MIP-1 beta
  • CCL5/RANTES
  • CXCL5/ENA-78
  • CXCL8/IL-8
  • FGF basic
  • G-CSF
  • GM-CSF
  • IFN-gamma
  • IL-1 alpha/IL-1F1
  • IL-1 beta/IL-1F2
  • IL-1ra/IL-1F3
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-17
  • TNF-alpha
  • Thrombopoietin/Tpo
  • VEGF

Human High Sensitivity Cytokine Panel A

  • CXCL8/IL-8
  • GM-CSF
  • IFN-gamma
  • IL-1 beta/IL-1F2
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-12 p70
  • TNF-alpha
  • VEGF

Human High Sensitivity Cytokine Panel B

  • GM-CSF
  • IFN-gamma
  • IL-1 beta/IL-1F2
  • IL-2
  • IL-5
  • IL-6
  • IL-7
  • IL-13
  • IL-15
  • IL-17A
  • IL-22
  • IL-23
  • IL-31
  • IL-33
  • IL-36 beta
  • TNF-alpha

Human Kidney Biomarker Panel

  • Clusterin
  • Cystatin C
  • CXCL10/IP-10
  • Lipocalin-2/NGAL
  • Osteopontin (OPN)
  • RBP4
  • TFF3
  • TIM-1/KIM-1/HAVCR

Human MMP Panel

  • MMP-1
  • MMP-2
  • MMP-3
  • MMP-7
  • MMP-8
  • MMP-9
  • MMP-10
  • MMP-12
  • MMP-13
  • EMMPRIN

Human Obesity Panel

  • Adiponectin/Acrp30
  • CCL2/MCP-1
  • Complement Factor D/Adipsin
  • C-Reactive Protein
  • IL-10
  • IL-6
  • Leptin
  • Resistin
  • Serpin E1/PAI-1
  • TNF-alpha

Human TIMP 4-plex - this kit is only available in the premixed format

  • TIMP-1
  • TIMP-2
  • TIMP-3
  • TIMP-4

Human XL Cytokine Discovery Panel

  • CCL2/MCP-1
  • CCL3/MIP-1 alpha
  • CCL4/MIP-1 beta
  • CCL5/RANTES
  • CCL11/Eotaxin
  • CCL19/MIP-3 beta
  • CCL20/MIP-3 alpha
  • CD40 Ligand
  • CX3CL1/Fractalkine
  • CXCL1/GRO alpha
  • CXCL2/GRO beta
  • CXCL10/IP-10
  • EGF
  • FGF basic
  • Flt-3 Ligand
  • G-CSF
  • GM-CSF
  • Granzyme B
  • IFN-alpha
  • IFN-beta
  • IFN-gamma
  • IL-1 alpha
  • IL-1 beta
  • IL-1ra/IL-1F3
  • IL-2
  • IL-3
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8/CXCL8
  • IL-10
  • IL-12 p70
  • IL-13
  • IL-15
  • IL-17A
  • IL-17E/IL-25
  • IL-33
  • PD-L1/B7-H1
  • PDGF-AA
  • PDGF-BB
  • TGF-alpha
  • TNF-alpha
  • TRAIL
  • VEGF

Multi-species TGF-beta Panel

  • TGF-beta 1
  • TGF-beta 2
  • TGF-beta 3

Analytes available in the polystyrene bead format

Human Adhesion Molecule 4-plex - this kit is only available in the premixed format

  • ICAM-1/CD54
  • E-Selectin/CD62E
  • P-Selectin/CD62P
  • VCAM-1/CD106

Human Angiogenesis Panel

  • Angiogenin
  • Angiopoietin-1
  • Endostatin
  • FGF acidic
  • FGF basic
  • PDGF-AA
  • PDGF-BB
  • PlGF
  • Thrombospondin-2
  • VEGF
  • VEGF-D

Human Biomarker Panel A

  • BAFF/BLyS
  • CCL20/MIP-3 alpha
  • CD14
  • CD27/TNFRSF7
  • CXCL13/BLC/BCA-1
  • gp130
  • IL-2 R alpha
  • IL-6 R alpha
  • TNF RII/TNFRSF1B

Human Cardiac Panel A

  • CD40 Ligand/TNFSF5
  • GDF-15
  • Pappalysin-1/PAPP-A
  • PCSK9
  • ST2/IL-33 R
  • TNF RII/TNFRSF1B

Human Cardiac Panel B

  • C-Reactive Protein/CRP
  • Cystatin C
  • Myeloperoxidase/MPO
  • P-Selectin/CD62P
  • Serpin E1/PAI-1
  • TIMP-1

Human Cytokine Panel A

  • CCL2/MCP-1
  • CCL3/MIP-1 alpha
  • CCL4/MIP-1 beta
  • CCL5/RANTES
  • CXCL5/ENA-78
  • CXCL8/IL-8
  • FGF basic
  • G-CSF
  • GM-CSF
  • IFN-gamma
  • IL-1 alpha/IL-1F1
  • IL-1 beta/IL-1F2
  • IL-1ra/IL-1F3
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-17
  • TNF-alpha
  • Thrombopoietin/Tpo
  • VEGF

Human Cytokine Panel B

  • CCL11/Eotaxin
  • CD40 Ligand/TNFSF5
  • CXCL10/IP-10
  • CXCL11/I-TAC
  • EGF
  • HGF
  • IL-12 p70
  • IL-13
  • Leptin/OB

Human High Sensitivity Cytokine Panel A

  • CXCL8/IL-8
  • GM-CSF
  • IFN-gamma
  • IL-1 beta/IL-1F2
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-12 p70
  • TNF-alpha
  • VEGF

Human MMP Panel

  • MMP-1
  • MMP-2
  • MMP-3
  • MMP-7
  • MMP-8
  • MMP-9
  • MMP-10
  • MMP-12
  • MMP-13
  • EMMPRIN

Human Obesity Panel

  • Adiponectin/Acrp30
  • C-Reactive Protein/CRP
  • CCL2/MCP-1
  • Comp. Factor D/Adipsin
  • IL-6
  • IL-10
  • Leptin/OB
  • Resistin
  • Serpin E1/PAI-1
  • TNF-alpha

Human TIMP 4-plex - this kit is only available in the premixed format

  • TIMP-1
  • TIMP-2
  • TIMP-3
  • TIMP-4

Multi-species TGF-beta Panel

  • TGF-beta 1
  • TGF-beta 2
  • TGF-beta 3