15-PGDH/HPGD Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87062

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 15-PGDH/HPGD Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit 15-PGDH/HPGD Antibody - BSA Free (NBP1-87062) is a polyclonal antibody validated for use in IHC and WB. Anti-15-PGDH/HPGD Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for 15-PGDH/HPGD Antibody - BSA Free

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human urinary bladder and cerebral cortex tissues using NBP1-87062 antibody. Corresponding HPGD RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human stomach shows moderate to strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [NBP1-87062] - Staining of human cerebral cortex shows no positivity in neurons as expected.
15-PGDH/HPGD Antibody - BSA Free Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody - BSA Free [NBP1-87062]

Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody - BSA Free [NBP1-87062]

Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Western Blot: 15-PGDH/HPGD Antibody [NBP1-87062] - Analysis in human cell line RT-4 and human cell line U-251 MG.
15-PGDH/HPGD Antibody - BSA Free Western Blot: 15-PGDH/HPGD Antibody - BSA Free [NBP1-87062]

Western Blot: 15-PGDH/HPGD Antibody - BSA Free [NBP1-87062]

Analysis using Anti-HPGD antibody NBP1-87062 (A) shows similar pattern to independent antibody NBP1-87061 (B).

Applications for 15-PGDH/HPGD Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 15-PGDH/HPGD

15-PGDH (15-hydroxyprostaglandin dehydrogenase (NAD+)) is responsible for prostaglandin inactivation and supports the regulation of functions that are controled by prostaglandin levels. Specifically, 15PGDH catalyzes the chemical reaction to convert NAD+ to NADH and H+.

15-PGDH is a novel tumor suppressor in the COX-2 pathway. 15-PGDH is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.

Long Name

15-Hydroxyprostaglandin Dehydrogenase

Alternate Names

15PGDH, HPGD, PGDH1, SDR36C1

Gene Symbol

HPGD

Additional 15-PGDH/HPGD Products

Product Documents for 15-PGDH/HPGD Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 15-PGDH/HPGD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 15-PGDH/HPGD Antibody - BSA Free

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Protocols

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