Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 4EBP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978).

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 4EBP1 Antibody - BSA Free

Knockout Validated: 4EBP1 Antibody [NB200-157]

Western Blot: 4EBP1 Antibody [NB200-157]

Western Blot: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: 4EBP1 Antibody [NB200-157]

Western Blot: 4EBP1 Antibody [NB200-157]

Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.
Immunohistochemistry: 4EBP1 Antibody [NB200-157]

Immunohistochemistry: 4EBP1 Antibody [NB200-157]

Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
4EBP1 Antibody

Western Blot: Rabbit Polyclonal 4EBP1 Antibody [NB200-157] -

Western Blot: Rabbit Polyclonal 4EBP1 Antibody [NB200-157] - Whole cell lysates from MDA-MB-231, SUM159 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. 4EBP1 Antibody (NB200-157) was used for primary antibody: 1:2000, 4C, overnight. Image from a verified customer review.

Applications for 4EBP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000-1:20000

Immunohistochemistry-Paraffin

1:5000-1:20000

Western Blot

1:1000-1:6000
Application Notes
For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended.

Reviewed Applications

Read 1 review rated 5 using NB200-157 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: 4EBP1

eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation.. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Entrez Gene IDs

1978 (Human); 13685 (Mouse); 116636 (Rat)

Gene Symbol

EIF4EBP1

UniProt

Q13541

Additional 4EBP1 Products

Product Documents for 4EBP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 4EBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

Citations for 4EBP1 Antibody - BSA Free

Customer Reviews for 4EBP1 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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Customer Images

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  • 4EBP1 Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Verified Customer | Posted 12/20/2023
    Western Blot: whole cell lysates from MDA-MB-231, SUM159 and MCF-7 cells were loaded with 50 ug/lane. 10% SDS-PAGE. 4EBP1 Antibody (NB200-157) was used for primary antibody: 1:2000, 4℃, overnight.
    4EBP1 Antibody - BSA Free NB200-157

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Protocols

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