5-HT3A Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56351

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Immunohistochemistry Free-Floating, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-HT3A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE) of rat 5-HT3 Receptor, accession number P35563.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT3A Antibody - BSA Free

Western Blot: 5-HT3A Antibody [NB100-56351]

Western Blot: 5-HT3A Antibody [NB100-56351]

5-HT3A-Antibody-Western-Blot-NB100-56351-img0013.jpg
Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351]

Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351]

Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.
Western Blot: 5-HT3A Antibody [NB100-56351]

Western Blot: 5-HT3A Antibody [NB100-56351]

Western Blot: 5-HT3A Antibody [NB100-56351] - Analysis of 5-HT3A in A: human, B: mouse and C: rat brain tissue lysate using 5-HT3A antibody at 3 ug/mL.
Simple Western: 5-HT3A Antibody [NB100-56351]

Simple Western: 5-HT3A Antibody [NB100-56351]

Simple Western: 5-HT3A Antibody [NB100-56351] - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
5-HT3A Antibody

Western Blot: 5-HT3A Antibody [NB100-56351] -

The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim.

Applications for 5-HT3A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

3 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 65 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT3A

Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems. Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids. The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse and rat receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord.

Long Name

5-Hydroxytryptamine Receptor 3A

Alternate Names

5HT3A, HTR3A

Entrez Gene IDs

15561 (Mouse); 79246 (Rat)

Gene Symbol

HTR3A

UniProt

P35563 (Rat)

Additional 5-HT3A Products

Product Documents for 5-HT3A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5-HT3A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5-HT3A Antibody - BSA Free

Customer Reviews for 5-HT3A Antibody - BSA Free

There are currently no reviews for this product. Be the first to review 5-HT3A Antibody - BSA Free and earn rewards!

Have you used 5-HT3A Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 5-HT3A Antibody - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Can you please let me know if you have antibodies against the following? For nAChR alpha 7 receptor of human origin - between amino acids 321- 465 For Glycine alpha1 receptor of human origin - between amino acids 339-413 For 5-HT 3A receptor of mouse origin - between amino acids 338-452

    A: We do not routinely epitope map our antibodies, and do not typically know where the antigen/antibody cites are. On occasion, customers will provide feedback about the epitopes, but this is not the norm. Unfortunately, we do not have any antibodies for nAChR alpha 7 receptor. For alpha 1 Glycine Receptor, we have two antibodies that target the N-terminus, NB300-113 and NBP1-49174, but I do not have more specific information about the epitope. For 5HT3A, we do have one antibody that may suit your needs, NB100-56351.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies