Q: Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.

A: We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

Q: We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?

A: We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.

53BP1 Antibody [DyLight 650]

Novus Biologicals | Catalog # NB100-305C

Submit a review

Citations (1)

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bat, Bovine, Canine, Naked mole-rat

Predicted:

Chimpanzee (100%), Equine (100%), Feline (100%), Hamster (100%), Orangutan (100%), Porcine (100%), Primate (100%), Rabbit (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Knockdown Validated

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Rabbit IgG

View all 53BP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to the C-terminal portion of the human 53BP1 protein (between amino acids 1925 and 1972) (NP_005648.1).

Reactivity Notes

Use in Naked mole-rat reported in scientific literature (PMID: 33608273). Human has been tested in both WB and ICC/IF, mouse has only been tested in ICC/IF. Feedback on bovine has been negative. Rat reactivity reported in scientific literature (PMID: 24244353). Bat, canine, and bovine reactivity reported in scientific literature (PMID: 27573809). Predicted cross-reactivity based on sequence identity: Chimpazee (100%), Equine (100%), Feline (100%), Gibbon (100%), Gorilla (100%), Hamster (100%), Marmoset (100%), Orangutan (100%), Panda (100%), Porcine (100%), Rabbit (100%), Sheep (100%).

Marker

DNA Double Strand Break Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 53BP1 Antibody [DyLight 650]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [DyLight 650] [NB100-305C] -

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody [DyLight 650] [NB100-305C] - Dual-color dSTORM acquisition workflow with fluorescence nanodiamonds (FNDs). (a) Representative dSTORM images of MCF10A cells upon targeting of 53BP1 (red) & DYNLL1 (green), stained with AlexaFluor647 & CF568, respectively. Shown are (from left to right) the single-color 53BP1 & DYNLL1 images & the dual-color dSTORM image. (b) FNDs spots positions obtained from red & green STORM channels. (c) Zoomed region of the highlighted area (white dashed box) in (b). Upper panel the localization events (each colored pixel represents one localized single-molecule event) recorded during the acquisition. Representative FNDs are indicated by the arrows. In the lower panel, the recorded events were transformed into Gaussian PSFs of fixed width & segmented (see Materials & Methods & Supplementary Protocol for details) to highlight the drift correction (Scale bar ROI: 1 μm) (d) Representative images of dual-color dSTORM acquisition. The fluorophore blinking in timelapse imaging (15,000 frames) for each channel (53BP1-AlexaFluor647 & DYNLL1-CF568) is shown. The bright spots in the image correspond to the fluorescence emission of individual fluorophores. Most of the fluorescent labels are switched off such that the fluorescent molecules are well separated. FNDs show no blinking properties, indicating high photostability in both channels. (e,f) Comparison of the photon emission from fluorescent nanodiamonds (FND, 40 nm; black), Alexa Fluor 647 dye (red) & CF568 dye (green) over 2000 acquired frames using 561 nm & 647 nm laser excitations, measured in the highlighted regions (white dashed box). (g) Fluorescence decrease of the 40 nm FNDs (black) & the AlexaFluor647 (red), over 15,000 frames of image. Scale bar: 3 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35215014), licensed under a CC-BY license. Not internally tested by Novus Biologicals.