A20/TNFAIP3 Antibody - BSA Free

Western Blot: A20/TNFAIP3 Antibody - BSA Free [NBP1-77024] -

A20 downregulation promoted necroptosis in vitro. (A) Primary neuron and astrocyte were obtained and verified in relative antibody. (B) A20 protein expression in primary neuron, primary astrocyte and HAPI. (C) Relative mRNA expression of A20 in different cell types was obtained from a database of Stanford University (https://web.stanford.edu/group/barres_lab/brain_rnaseq.html). (D) A20 was significantly decreased after si-A20 treatment in the three cell types, including primary neuron, primary astrocyte and HAPI. (E,F) Tumor necrosis factor (TNF)-alpha (10 ng/ml) + Z-VAD (100 μm) combination (TZ) as a necroptosis inducer was used for 24 h. In primary neuron, RIP1, RIP3 and MLKL were tested by western blot. Data were analyzed by statistical. (G,H) TUNEL assays and (I) CCK-8 (n = 4) were used to test cell death and viability of neuron after 24 h treatment. (J–M) Western blot assay was used to test RIP1, RIP3, MLKL, NF-KB and relative inflammatory factors in primary HAPI and astrocyte respectively. Data were measured by one way ANOVA plus Tukey’s test. *P < 0.05 and **P < 0.01 vs. TZ group; #P < 0.05 and ##P < 0.01 vs. TZ+si-NC group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: A20/TNFAIP3 Antibody - BSA Free [NBP1-77024] -

A20 downregulation promoted necroptosis in vitro. (A) Primary neuron and astrocyte were obtained and verified in relative antibody. (B) A20 protein expression in primary neuron, primary astrocyte and HAPI. (C) Relative mRNA expression of A20 in different cell types was obtained from a database of Stanford University (https://web.stanford.edu/group/barres_lab/brain_rnaseq.html). (D) A20 was significantly decreased after si-A20 treatment in the three cell types, including primary neuron, primary astrocyte and HAPI. (E,F) Tumor necrosis factor (TNF)-alpha (10 ng/ml) + Z-VAD (100 μm) combination (TZ) as a necroptosis inducer was used for 24 h. In primary neuron, RIP1, RIP3 and MLKL were tested by western blot. Data were analyzed by statistical. (G,H) TUNEL assays and (I) CCK-8 (n = 4) were used to test cell death and viability of neuron after 24 h treatment. (J–M) Western blot assay was used to test RIP1, RIP3, MLKL, NF-KB and relative inflammatory factors in primary HAPI and astrocyte respectively. Data were measured by one way ANOVA plus Tukey’s test. *P < 0.05 and **P < 0.01 vs. TZ group; #P < 0.05 and ##P < 0.01 vs. TZ+si-NC group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: A20/TNFAIP3 Antibody - BSA Free [NBP1-77024] -

AAV-shA20 attenuated the anti-necroptotic roles of Nec-1 and melatonin and recovered the RAGE/NF-kappa B pathway. (A,B) In the cortex tissues, AAV-shA20 inhibited the effect of Nec-1 and melatonin on RIP1, RIP3 and MLKL, determined by immunoblotting and immunohistochemistry. beta -actin was used as a control. (C,D) The same results were demonstrated in hippocampal CA1 tissues. (E,F) Restored RAGE and active NF-kappa B pathway were detected in cortex tissues by immunoblotting after AAV-shA20 administration. The same results were demonstrated in hippocampal CA1 tissues. beta -actin was used as a control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: A20/TNFAIP3 Antibody - BSA Free [NBP1-77024] -

AAV-shA20 attenuated the anti-necroptotic roles of Nec-1 and melatonin and recovered the RAGE/NF-kappa B pathway. (A,B) In the cortex tissues, AAV-shA20 inhibited the effect of Nec-1 and melatonin on RIP1, RIP3 and MLKL, determined by immunoblotting and immunohistochemistry. beta -actin was used as a control. (C,D) The same results were demonstrated in hippocampal CA1 tissues. (E,F) Restored RAGE and active NF-kappa B pathway were detected in cortex tissues by immunoblotting after AAV-shA20 administration. The same results were demonstrated in hippocampal CA1 tissues. beta -actin was used as a control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.