ABCA4 Antibody (3F4) - Azide Free

Novus Biologicals | Catalog # NBP1-30032

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Feline, Xenopus

Cited:

Bovine, Canine, Frog - Xenopus (African Clawed Frog)

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3F4

Format

Azide Free
View all ABCA4 Primary Antibodies »

Product Specifications

Immunogen

Partially purified bovine 220-kDa disc rim protein. Accession # F1MWM0

Epitope

PLHPRTAGASR (PMID: 9092582)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Canine reactivity reported in scientific literature (PMID: 30889179). Feline reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABCA4 Antibody (3F4) - Azide Free

Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032]

Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032]

Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032]

Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032]

Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.
Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032]

Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032]

ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0003.jpg
Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032]

Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032]

ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0004.jpg
Knockout Validated: ABCA4 Antibody (3F4) [NBP1-30032]

Western Blot: ABCA4 Antibody (3F4) [NBP1-30032]

ABCA4-Antibody-3F4-Western-Blot-NBP1-30032-img0002.jpg
Knockout Validated: ABCA4 Antibody (3F4) [NBP1-30032]

Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032]

ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0005.jpg
ABCA4 Antibody (3F4)

Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] -

Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression & western blot analyses of ABCA4 protein levels in the canine retina.(A) Relative ABCA4 mRNA expression levels by quantitative RT-PCR in three different regions in three dogs with different genotypes (ABCA4+/+, ABCA4+/-, & ABCA4-/-), normalized to GAPDH expression. (B) Western blot analyses of ABCA4 (above), GAPDH (middle), & RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ABCA4 Antibody (3F4)

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] -

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ABCA4 Antibody (3F4)

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] -

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ABCA4 Antibody (3F4)

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] -

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ABCA4 Antibody (3F4)

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] -

Immunocytochemistry/ Immunofluorescence: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, & autofluorescence in the canine retina.(A-C) Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), & DAPI nuclear staining (blue) in wild-type (ABCA4+/+), heterozygous (ABCA4+/-), & affected (ABCA4-/-) retinas. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. (D) Bar graph with the average number of DAPI-stained nuclei within a given region of the ONL & the INL. (E-G) Fluorescence micrographs of RPE without immunohistochemistry show autofluorescence. (H) Bar graph with background-corrected mean autofluorescence-intensity in the RPE. Note the reduction of ABCA4-immunoreactivity & PNA binding, higher autofluorescence, & fewer nuclei in the ONL in the ABCA4-/- compared to ABCA4+/+ or ABCA4+/- retinas. All scale bars = 50 μm; RPE = retinal pigment epithelium; ONL = outer nuclear layer; INL = inner nuclear layer; Because there was only one individual per genotype, the statistics are valid for the technical replicates. ANOVA with Tukey’s post hoc test, n = 6; **P < 0.01; ***P < 0.001; mean ± S.D. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30889179), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ABCA4 Antibody (3F4) - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:10 - 1:500

Western Blot

1:1000
Application Notes
Although not confirmed, this product may be useful in Immunohistochemistry-Paraffin. Immunohistochemistry-Frozen was reported in scientific literature. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30889179).

Reviewed Applications

Read 1 review rated 5 using NBP1-30032 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABCA4

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essental molecule across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease, a form of juvenile-onset macular degeneration. Mutations in this gene are also associated with retinitis pigmentosa-19, cone-rod dystrophy type 3, early-onset severe retinal dystrophy, fundus flavimaculatus, and macular degeneration age-related 2.

Alternate Names

ABC10, ABCRRMP, ARMD2DKFZp781N1972, ATP binding cassette transporter, ATP-binding cassette sub-family A member 4, ATP-binding cassette transporter, retinal-specific, ATP-binding cassette, sub-family A (ABC1), member 4, ATP-binding transporter, retina-specific, CORD3, EC 3.6.3, FFMFLJ17534, photoreceptor rim protein, retinal-specific ATP-binding cassette transporter, retina-specific ABC transporter, RIM ABC transporter, RIM protein, RmP, RP19, Stargardt disease protein, STGD, STGD1

Entrez Gene IDs

24 (Human); 11304 (Mouse)

Gene Symbol

ABCA4

Additional ABCA4 Products

Product Documents for ABCA4 Antibody (3F4) - Azide Free

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Product Specific Notices for ABCA4 Antibody (3F4) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ABCA4 Antibody (3F4) - Azide Free

Customer Reviews for ABCA4 Antibody (3F4) - Azide Free (1)

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Customer Images

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  • ABCA4 Antibody (3F4)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: cat retina
    Species: Feline
    Verified Customer | Posted 09/25/2020
    IHC of normal cat retina with ABCA4 3F4 (red) and HCAR (green). ABCA4 (1:1000) labels outer segments of photoreceptors and HCAR labels cones.
    Cat retina was fixed in PFA, embedded in OCT and frozen in liquid nitrogen. OCT block was sectioned (10um) and subjected to IHC.
    ABCA4 Antibody (3F4) - Azide Free NBP1-30032

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