Abhd5 Antibody (1F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00051099-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Monkey

Cited:

Human, Mouse, Bovine

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F3

Format

Azide and BSA Free
View all Abhd5 Primary Antibodies »

Product Specifications

Immunogen

ABHD5 (AAH21958, 1 a.a. ~ 349 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQRIGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD

Reactivity Notes

Mouse reactivity reported in multiple pieces of scientific literature

Specificity

ABHD5 - abhydrolase domain containing 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Abhd5 Antibody (1F3) - Azide and BSA Free

Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01]

Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01]

Immunohistochemistry-Paraffin: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 expression in HeLa, mouse and bovine tissues. Primary: 1:500 dilution. This image was submitted via customer Review.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in Jurkat(Cat # L017V1 ).
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Western Blot analysis of ABHD5 expression in human liver.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in HeLa.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - ABHD5 monoclonal antibody (M01), clone 1F3. Analysis of ABHD5 expression in rat testis.
Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01]

Western Blot: Abhd5 Antibody (1F3) [H00051099-M01] - Analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody (M01), clone 1F3.Lane 1: ABHD5 transfected lysate(39 KDa).Lane 2: Non-transfected lysate.
ELISA: Abhd5 Antibody (1F3) [H00051099-M01]

ELISA: Abhd5 Antibody (1F3) [H00051099-M01]

ELISA: Abhd5 Antibody (1F3) [H00051099-M01] - Detection limit for recombinant GST tagged ABHD5 is approximately 1ng/ml as a capture antibody.
Abhd5 Antibody (1F3) - Azide and BSA Free

Western Blot: Abhd5 Antibody (1F3) - Azide and BSA Free [H00051099-M01] -

The Role of MAGL and CGI-58 in IL-6 and beta -adrenergic Adipocyte Signaling and Lipolysis. (A-D) Pre-adipocytes in a 12-well format were infected with lentivirus containing Cas9 and sgGFP (black), sgAbhd5 (red), or sgMgll (blue) followed by differentiation as described in Methods. On day 8, differentiated adipocytes were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS containing the indicated concentration of isoproterenol (A, B), IL-6 (A, C), or LIF (A, D). After incubation for 24 hours, medium was collected and processed to measure concentrations of glycerol (left panel) or NEFA (right panel) and adipocytes were harvested and processed for immunoblot analysis (A) with the indicated antibodies as described in Methods. Glycerol levels are shown relative to the average 300 nM isoproterenol condition (B, 653 uM), 3 nM IL-6 condition (C, 109 uM), or 0.3 nM LIF condition (D, 55 uM) of the sgGFP-treated adipocytes. NEFA levels are shown relative to the average 300 nM isoproterenol condition (B, 282 uM), 3 nM IL-6 condition (C, 70 uM), or 1 nM LIF condition (D, 21 uM) of the sgGFP-treated adipocytes. Data is shown as mean +/- SEM of three wells. P value calculated by using a two-way repeated measures ANOVA between sgGFP and the indicated sgRNA conditions. These results were confirmed in at least three independent experiments. Not significant (n.s.). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Abhd5 Antibody (1F3) - Azide and BSA Free

Western Blot: Abhd5 Antibody (1F3) - Azide and BSA Free [H00051099-M01] -

Comparison of IL-6 Family Cytokine and beta -adrenergic Adipocyte Signaling and Lipolysis. (A–D) Differentiated adipocytes in a 12-well format were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS in the absence or presence of 300 nM isoproterenol (black, Isop.), 3 nM IL-6 (red), or 0.3 nM LIF (blue). After incubation (37C, 10% CO2) for either 24 hours (A, B) or the indicated time (C, D), medium was collected and processed to measure concentrations of NEFA (A, C; top panels) or glycerol (A,C; bottom panels), and adipocytes were harvested and processed for immunoblot analysis (B, D) with the indicated antibody as described in Methods. Data is shown as mean +/- SEM of three wells. *p < 0.05, **p < 0.01, or ****p < 0.0001 based on one-way ANOVA followed by Dunnett’s multiple comparison test to compare each condition to the vehicle condition (A) or two-way ANOVA followed by Šidak’s multiple comparison test to compare isoproterenol to IL-6 conditions at matched time points (B). These results were confirmed in at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Abhd5 Antibody (1F3) - Azide and BSA Free

Western Blot: Abhd5 Antibody (1F3) - Azide and BSA Free [H00051099-M01] -

Comparison of IL-6 Family Cytokine and beta -adrenergic Adipocyte Signaling and Lipolysis. (A–D) Differentiated adipocytes in a 12-well format were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS in the absence or presence of 300 nM isoproterenol (black, Isop.), 3 nM IL-6 (red), or 0.3 nM LIF (blue). After incubation (37C, 10% CO2) for either 24 hours (A, B) or the indicated time (C, D), medium was collected and processed to measure concentrations of NEFA (A, C; top panels) or glycerol (A,C; bottom panels), and adipocytes were harvested and processed for immunoblot analysis (B, D) with the indicated antibody as described in Methods. Data is shown as mean +/- SEM of three wells. *p < 0.05, **p < 0.01, or ****p < 0.0001 based on one-way ANOVA followed by Dunnett’s multiple comparison test to compare each condition to the vehicle condition (A) or two-way ANOVA followed by Šidak’s multiple comparison test to compare isoproterenol to IL-6 conditions at matched time points (B). These results were confirmed in at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Abhd5 Antibody (1F3) - Azide and BSA Free

Western Blot: Abhd5 Antibody (1F3) - Azide and BSA Free [H00051099-M01] -

The Role of ATGL and HSL in IL-6 and beta -adrenergic Adipocyte Signaling and Lipolysis. (A–D) Pre-adipocytes in a 12-well format were infected with lentivirus containing Cas9 and sgGFP (black), sgPnpla2 (red), or sgLipe (blue) followed by differentiation as described in Methods. On day 8, differentiated adipocytes were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS containing the indicated concentration of isoproterenol (A, B), IL-6 (A, C), or LIF (A, D). After incubation for 24 hours, medium was collected and processed to measure concentrations of glycerol (left panel) or NEFA (right panel) and adipocytes were harvested and processed for immunoblot analysis (A) with the indicated antibodies as described in Methods. Glycerol levels are shown relative to the average 300 nM isoproterenol condition (B, 578 uM), 3 nM IL-6 condition (C, 140 uM), or 1 nM LIF condition (D, 86 uM) of the sgGFP-treated adipocytes. NEFA levels are shown relative to the average 300 nM isoproterenol condition (B, 123 uM), 3 nM IL-6 condition (C, 57 uM), or 1 nM LIF condition (D, 27 uM) of the sgGFP-treated adipocytes. Data is shown as mean +/- SEM of three wells. P value calculated by using a two-way repeated measures ANOVA between sgGFP and the indicated sgRNA conditions. These results were confirmed in at least three independent experiments. Not significant (n.s.). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Abhd5 Antibody (1F3) - Azide and BSA Free

Western Blot: Abhd5 Antibody (1F3) - Azide and BSA Free [H00051099-M01] -

The Role of STAT3 in IL-6 and beta -adrenergic Adipocyte Signaling and Lipolysis. (A–C) Pre-adipocytes in a 12-well format were infected with lentivirus containing Cas9 and the indicated sgRNA as described in Methods. On day 8, differentiated adipocytes were treated in a final volume of 1 ml of medium D with 3 ul DMSO and 3 ul PBS containing 300 nM isoproterenol, 3 nM IL-6, or 0.3nM LIF. After incubation for 24 hours, medium was collected and processed to measure concentrations of glycerol (B) or NEFA (C) and adipocytes were harvested and processed for immunoblot analysis (A) with the indicated antibodies as described in Methods. Glycerol release is shown relative to the respective stimulant in the sgGFP condition which were 366 μM, 70 μM, and 33 μM for isoproterenol, IL-6, and LIF, respectively. NEFA release is shown relative to the respective stimulant in the sgGFP condition which were 273 μM, 137 μM, and 34 μM for isoproterenol, IL-6, and LIF, respectively. Data is shown as mean +/- SEM of three wells. *p < 0.5, ***p < 0.001, or ****p < 0.0001 based on two-way ANOVA with Tukey’s multiple comparison test for differences in relative glycerol or NEFA release between sgRNA treatments with the same stimulant. These results were confirmed in at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35785158), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Abhd5 Antibody (1F3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell, tissue and transfected lysate for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Knock Out Validation was reported in scientific literature (PMID: 27559856). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 25894224).

Reviewed Applications

Read 1 review rated 3 using H00051099-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Abhd5

The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation.

Alternate Names

abhydrolase domain containing 5, Abhydrolase domain-containing protein 5,1-acylglycerol-3-phosphate O-acyltransferase ABHD5, CDS, CGI58, EC 2.3.1.51, IECN2, Lipid droplet-binding protein CGI-58, MGC8731, NCIE2CGI-58

Entrez Gene IDs

51099 (Human)

Gene Symbol

ABHD5

OMIM

275630 (Human)

UniProt

AAH21958 (Human)

Additional Abhd5 Products

Product Documents for Abhd5 Antibody (1F3) - Azide and BSA Free

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Product Specific Notices for Abhd5 Antibody (1F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Abhd5 Antibody (1F3) - Azide and BSA Free

Customer Reviews for Abhd5 Antibody (1F3) - Azide and BSA Free (1)

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Customer Images

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  • Abhd5 Antibody (1F3)
    Name: Heather Talbott
    Application: Western Blot
    Sample Tested: Hela whole cell lysate, Mouse adipose tissue, mouse liver lysate, bovine adipose tissue lysate, bovine liver tissue lysate and HeLa Cytosol
    Species: Bovine, Mouse and Human
    Verified Customer | Posted 09/12/2017
    Western Blot analysis of ABHD5 expression in HeLa, mouse and bovine tissues.
    4-20% gradient gel 15 ug/well PVDF and Nitocellulose Block: 5% NFDM in TBS 0.1% Tween-20 Primary: 1:500 dilution, 1% NFDM in TBS 0.1% Tween-20, overnight at 4 C Secondary: 1:10,000 anti-mouse - HRP (Jackson ImmunoResearch 115-035-205); 1:5000 anti-biotin - HRP (Cell Signaling 7075P5), 1% NFDM in TBS 0.1% Tween-20, 1 hr at RT ECL: SuperSignal West Femto (ThermoFisher Scientific 34096), UVP Image Station
    Abhd5 Antibody (1F3) - Azide and BSA Free H00051099-M01

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