ACAT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89284

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Human, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACAT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NEQDAYAINSYTRSKAAWEAGKFGNEVIPVTVTVKGQPDVVVKEDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLARIVAFADAAVEPIDFPIAPVYAASMV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (81%). Reactivity reported in scientific literature (PMID: 22361696). Porcine reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACAT1 Antibody - BSA Free

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).
Western Blot: ACAT1 Antibody [NBP1-89284]

Western Blot: ACAT1 Antibody [NBP1-89284]

Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Western Blot: ACAT1 Antibody [NBP1-89284]

Western Blot: ACAT1 Antibody [NBP1-89284]

Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).
Immunocytochemistry/ Immunofluorescence: ACAT1 Antibody [NBP1-89284]

Immunocytochemistry/ Immunofluorescence: ACAT1 Antibody [NBP1-89284]

Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.
Western Blot: ACAT1 Antibody [NBP1-89284]

Western Blot: ACAT1 Antibody [NBP1-89284]

Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.
Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.

Applications for ACAT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization, Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP1-89284 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAT1

Sterol O-acyltransferase 1 (SOAT1), also known as ACAT1, catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys. Specifically, ACAT-1 catalyzes the reversible reaction forming acetoacetyl-CoA from two molecules of acetyl-CoA. Additionaly, the ACAT 1 protein may have a role in lipoprotein assembly and dietary cholesterol absorption, and may also act as a ligase.

Mutations in this gene have been associated with 3-ketothiolase deficiency, an inborn defect of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Long Name

Acetyl-CoA Acetyltransferase 1

Alternate Names

THIL

Gene Symbol

ACAT1

Additional ACAT1 Products

Product Documents for ACAT1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ACAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bioactive Lipids and Receptors

Citations for ACAT1 Antibody - BSA Free

Customer Reviews for ACAT1 Antibody - BSA Free (1)

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  • ACAT1 Antibody
    Name: Jennifer McGuire
    Application: Western Blot
    Sample Tested: brain cortical homogenate and Brain (cerebral cortex)
    Species: Pig
    Verified Customer | Posted 07/24/2018
    ACAT1 porcine cerebral cortex, 5ug lane 1, 10ug lane 2
    primary concentration 1:500, Secondary concentration 1:10,000
    ACAT1 Antibody - BSA Free NBP1-89284

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Protocols

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