Acidic Calponin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38643

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Acidic Calponin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PKYCAAPTEPVIHNGSQGTGTNGSEISDSDYQAEYPDEYHGEYQDDYPRDYQYSDQGIDY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Acidic Calponin Antibody - BSA Free

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643] - Analysis in human placenta and skeletal muscle tissues. Corresponding Acidic Calponin RNA-seq data are presented for the same tissues.
Acidic Calponin Antibody

Western Blot: Acidic Calponin Antibody [NBP2-38643] -

Western Blot: Acidic Calponin Antibody [NBP2-38643] - Analysis in human cell lines U2OS and MCF-7 using Anti-CNN3 antibody. Corresponding CNN3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/ Immunofluorescence: Acidic Calponin Antibody [NBP2-38643]

Immunocytochemistry/ Immunofluorescence: Acidic Calponin Antibody [NBP2-38643]

Immunocytochemistry/Immunofluorescence: Acidic Calponin Antibody [NBP2-38643] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments.
Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643] - Staining of human testis shows weak to moderate positivity in peritubuler myoid cells.
Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643] - Staining of human kidney shows weak to moderate membranous and cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643] - Staining of human placenta shows strong positivity in endothelial cells.
Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643]

Immunohistochemistry-Paraffin: Acidic Calponin Antibody [NBP2-38643] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Acidic Calponin Antibody

Immunoprecipitation: Acidic Calponin Antibody [NBP2-38643] -

Immunoprecipitation: Acidic Calponin Antibody [NBP2-38643] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 μg of Acidic Calponin Antibody (NBP2-38643) pre-coupled to Dynabeads protein A. Samples were washed and processed for Western Blot with Acidic Calponin Antibody. For Western Blot, Acidic Calponin Antibody was used at 1/1000. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Acidic Calponin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Acidic Calponin

Acidic Calponin, or CNN3 for short, consists of a 329 amino acid isoform that is 36 kDa, and is involved in the regulation of smooth muscle contraction. Current research is being conducted on the relationship between acidic calponin and several diseases and disorders, including aortic aneurysm and neuronitis. The protein is not associated with any pathways, but it does interact with the proteins FYN, SLX4, ATG5, LCP1, and EMD.

Alternate Names

calponin 3, acidic, Calponin, acidic isoform, calponin-3, dJ639P13.2.2 (acidic calponin 3)

Gene Symbol

CNN3

UniProt

Q15417 (Human)

Additional Acidic Calponin Products

Product Documents for Acidic Calponin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Acidic Calponin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Acidic Calponin Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Acidic Calponin Antibody - BSA Free and earn rewards!

Have you used Acidic Calponin Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies