ACP2 Antibody (M1-4A12) - Azide and BSA Free

Novus Biologicals | Catalog # H00000053-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Lambda Clone # M1-4A12

Format

Azide and BSA Free
View all ACP2 Primary Antibodies »

Product Specifications

Immunogen

ACP2 (AAH03160, 1 a.a. ~ 423 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGKRSGWSRAALLQLLLGVNLVVMPPTQARSLRFVTLLYRHGDRSPVKTYPKDPYQEEEWPQGFGQLTKEGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNGMQRFNPNISWQPIPVHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNESSRNAQFLDMVANETGLTDLTLETVWNVYDTLFCEQTHGLRLPPWASPQTMQRLSRLKDFSFRFLFGIYQQAEKARLQGGVLLAQIRKNLTLMATTSQLPKLLVYSAHDTTLVALQMALDVYNGEQAPYASCHIFELYQEDSGNFSVEMYFRNESDKAPWPLSLPGCPHRCPLQDFLRLTEPVVPKDWQQECQLASGPADTEVIVALAVCGSILFLLIVLLLTVLFRMQAQPPGYRHVADGEDHA

Specificity

ACP2 - acid phosphatase 2, lysosomal

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ACP2 Antibody (M1-4A12) - Azide and BSA Free

Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01]

Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01]

Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.Lane 1: ACP2 transfected lysate(48.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: ACP2 Antibody (M1-4A12) [H00000053-M01]

Immunocytochemistry/ Immunofluorescence: ACP2 Antibody (M1-4A12) [H00000053-M01]

Immunocytochemistry/Immunofluorescence: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ACP2 Antibody (M1-4A12) [H00000053-M01]

Immunohistochemistry-Paraffin: ACP2 Antibody (M1-4A12) [H00000053-M01]

Immunohistochemistry-Paraffin: ACP2 Antibody (M1-4A12) [H00000053-M01] - Analysis of monoclonal antibody to ACP2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 5 ug/ml.
Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01]

Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01]

Western Blot: ACP2 Antibody (M1-4A12) [H00000053-M01] - ACP2 over-expressed 293 cell line. Lane 2 cotransfected with ACP2 Validated Chimera RNAi. Lane 1 non-transfected control. Blot probed with H00000053-M01. GAPDH (36.1 kDa) used as loading control.
ELISA: ACP2 Antibody (M1-4A12) [H00000053-M01]

ELISA: ACP2 Antibody (M1-4A12) [H00000053-M01]

ELISA: ACP2 Antibody (M1-4A12) [H00000053-M01] - Detection limit for recombinant GST tagged ACP2 is 1 ng/ml as a capture antibody.

Applications for ACP2 Antibody (M1-4A12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACP2

Lysosomal acid phosphatase is comprised of two subunits, alpha and beta, and is chemically and genetically distinct from red cell acid phosphatase. Lysosomal acid phosphatase 2 is a member of a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Acid phosphatase deficiency is caused by mutations in the ACP2 (beta subunit) and ACP3 (alpha subunit) genes.

Alternate Names

acid phosphatase 2, lysosomal, EC 3.1.3.2, LAP, lysosomal acid phosphatase

Entrez Gene IDs

53 (Human)

Gene Symbol

ACP2

OMIM

171650 (Human)

UniProt

AAH03160 (Human)

Additional ACP2 Products

Product Documents for ACP2 Antibody (M1-4A12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ACP2 Antibody (M1-4A12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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