ACSL1 Antibody - BSA Free

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• ACSL1 Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Frozen

  Sample Tested: brain tissue (hippocampus)

  Species: Rat

  Verified Customer | Posted 12/22/2017

  4% paraformaldehyde solution

  Name: S100A10 antibody Catalog #: NBP1-89370 Lot Number: A40997 Antibody Storage Conditions:-20℃ Application Tested: IHC-Fr Staining Expectations: co-labell A2 astrocytes with gfap Observation & Concerns: The background is very deep and the A2 astrocyte can’t be stained. Test Sample Information: Species & Treatments: SD rats & infrasound models Tissue: brain tissue (hippocampus) Fixative Composition: 4% paraformaldehyde solution Fixation Time & Temperature: 6 h or longer Tissue Processing: antigen retrieval, EDTA pH9.0, 95C, 15min Blocking Procedure: Blocking Solution: 3%BSA/5%BSA Time & Temperature: 0.5 or 1 h & room temperature Blocking Time: Click here to enter text. Primary Antibody Dilution: 1:25; 1:50; 1:100; 1:200; 1:500 Diluent Buffer: 3%BSA，0.3%Triton Time & Temperature: 12 h & 4℃ Washing Conditions: Wash Buffer Composition: 1 * PBS Method (Immersion, Rinsing etc.): Immersion Times/Washing: 3 * 10 min Repetitions: 3 times Secondary Antibody Manufacturer and Catalog #: CWBIO & CW0114S Secondary description: anti-rabbit secondary antibody is available, see section controls and additional images. Dilution: 1:200 Diluent Buffer: 1 * PBS(hoechest, PH=7.4) Incubation Time & Temperature: 2 h & room temperature Post-Secondary Washing: the same as post-primary washing Detection Method: Detection: DM4000 Controls: Positive Control: the anti-rabbit secondary antibody is available Negative Control: the production can not co-labell A2 ast

  

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