Actin Gamma 1 Antibody (NH3) - BSA Free

Novus Biologicals | Catalog # NB100-64792

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # NH3

Format

BSA Free
View all Actin Gamma 1 Primary Antibodies »

Product Specifications

Immunogen

Human monocytes and U937 cell line

Reactivity Notes

Predicted cross-reactivities: Rat, Rabbit, Mouse

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Actin Gamma 1 Antibody (NH3) - BSA Free

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunocytochemistry/Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.
Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792]

Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792] - Human inner ear tissue section stained with Actin Gamma 1 antibody at 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse at 1:200. Nuclei stained with DAPI. IHC-P image submitted by a verified customer review.
Actin Gamma 1 Antibody (NH3) - BSA Free

Western Blot: Actin Gamma 1 Antibody (NH3) - BSA Free [NB100-64792] -

Effect of TSAIII on cell migration, invasion, and F-actin expression of human osteosarcoma cells. (A) Human 143-B and HOS osteosarcoma cells were treated with various concentrations of timosaponin AIII (TSAIII; 0, 2, 4, and 6 μM) for 24 h, and cell migration and invasion abilities were measured. (B) Cytoskeletal F-actin expression in human 143-B and HOS osteosarcoma cells exposed to TSAIII (0, 2, 4, 6 μM) was measured through immunoblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. (C) Distribution of cytoskeletal F-actin in 143-B and HOS cells was further confirmed using immunofluorescence analysis. * p < 0.05; ** p < 0.01 versus control. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33799345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Actin Gamma 1 Antibody (NH3) - BSA Free

Western Blot: Actin Gamma 1 Antibody (NH3) - BSA Free [NB100-64792] -

Synergistically inhibitory effect of TSAIII and PF on migration and invasion of human osteosarcoma cells. (A) Human 143-B and HOS osteosarcoma cells were treated with various concentrations of TSAIII (0 and 4 μM) and/or PF (0 and 2 μM) and then harvested to detect the expression and activation of cytoskeletal-related proteins through immunoblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. (B) Using immunofluorescence analysis, the expression of cytoskeletal F-actin was observed in human 143-B and HOS osteosarcoma cells. (C) The migration and invasion abilities of human 143-B and HOS osteosarcoma cells were measured after treatment with TSAIII in the presence or absence of PF for 24 h. ** p < 0.01 versus control; # p < 0.05 versus treatment with PF alone (mean +/- standard error, n = 3). PF denotes PF-573228 (focal adhesion kinase inhibitor). Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33799345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Actin Gamma 1 Antibody (NH3) - BSA Free

Western Blot: Actin Gamma 1 Antibody (NH3) - BSA Free [NB100-64792] -

Synergistically inhibitory effect of TSAIII and Cyclo on migration and invasion of human osteosarcoma cells. (A) Human 143-B and HOS osteosarcoma cells were treated with various concentrations of TSAIII (0 and 4 μM) and/or Cyclo (0 and 50 μM) and then harvested to detect the expression and activation of cytoskeletal-related proteins through immunoblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. (B) A change in the expression of cytoskeletal F-actin was observed in human 143-B and HOS osteosarcoma cells using immunofluorescence analysis. (C) The migration and invasion capacities of human 143-B and HOS osteosarcoma cells were measured after treatment with TSAIII in the presence or absence of Cyclo for 18 h (migration) or 24 h (invasion). ** p < 0.01 versus control; # p < 0.05 versus treatment with Cyclo alone. Cyclo denotes Cyclo(RGDyK) (Intergin inhibitor). Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33799345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Actin Gamma 1 Antibody (NH3) - BSA Free

Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (NH3) - BSA Free [NB100-64792] -

Effect of TSAIII on cell migration, invasion, and F-actin expression of human osteosarcoma cells. (A) Human 143-B and HOS osteosarcoma cells were treated with various concentrations of timosaponin AIII (TSAIII; 0, 2, 4, and 6 μM) for 24 h, and cell migration and invasion abilities were measured. (B) Cytoskeletal F-actin expression in human 143-B and HOS osteosarcoma cells exposed to TSAIII (0, 2, 4, 6 μM) was measured through immunoblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. (C) Distribution of cytoskeletal F-actin in 143-B and HOS cells was further confirmed using immunofluorescence analysis. * p < 0.05; ** p < 0.01 versus control. Scale bar: 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33799345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Actin Gamma 1 Antibody (NH3) - BSA Free

Immunohistochemistry: Actin Gamma 1 Antibody (NH3) - BSA Free [NB100-64792] -

In vivo metastasis of TSAIII in human osteosarcoma cells. For the animal assay of lung metastasis, human osteosarcoma cells were harvested and injected into the tail veins of five-week-old immunodeficient mice (C.B17/IcrPrkdcscid/CrlNarl). The mice were then fed TSAIII (5 and 10 mg/kg) through oral gavage. After two months, the mice were euthanised and (A) the histopathology of the lungs in metastatic tumour-bearing animals was analysed. The lungs were fixed in neutral-buffered formalin and stained with haematoxylin and eosin. The F-actin expression were detected with immunohistochemistry assay. (B) The nodule numbers were then counted in the mice. ** p < 0.01 versus control (n = 5). Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33799345), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Actin Gamma 1 Antibody (NH3) - BSA Free

Application
Recommended Usage

ELISA

1:10

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Western Blot

1:100 - 1:500
Application Notes
Actin Gamma 1 antibody validated for ICC/IF and IHC-P from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 4 using NB100-64792 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Actin Gamma 1

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Alternate Names

ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin

Entrez Gene IDs

71 (Human)

Gene Symbol

ACTG1

UniProt

P63261 (Human)

Additional Actin Gamma 1 Products

Product Documents for Actin Gamma 1 Antibody (NH3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Actin Gamma 1 Antibody (NH3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Actin Gamma 1 Antibody (NH3) - BSA Free

Customer Reviews for Actin Gamma 1 Antibody (NH3) - BSA Free (2)

4 out of 5
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Actin Gamma 1 Antibody (NH3)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: inner ear
    Species: Human
    Verified Customer | Posted 10/30/2019
    Human inner ear tissue was incubated with anti-actin antibody at dilution 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse at dilution 1:200. Nuclei stained with DAPI.
    Actin Gamma 1 Antibody (NH3) - BSA Free NB100-64792
  • Name: Chan-Yen Kuo
    Application: Immunofluorescence
    Sample Tested:
    Species: Human
    Verified Customer | Posted 07/05/2013
    The distribution of stress fiber was observed by F-actin staining (Green, Novus)
    Actin Gamma 1 Antibody (NH3) - BSA Free NB100-64792

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