Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ADAM10 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ADAM10. The immunogen is located within the last 50 amino acids of ADAM10. Amino Acid Squence: PQRQRPRESYQMGHMRR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ADAM10 Antibody - BSA Free

ADAM10 Antibody - BSA Free

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] -

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - KO Validation in MEF Cells.Loading: 10 u of lysate Antibodies: ADAM10, 1 u/mL and beta-actin, 1u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).
ADAM10 Antibody - BSA Free

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] -

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - KO Validation in 293 Cells.Loading: 15 u of lysate Antibodies: ADAM10, 2 u/mL and beta-actin, 1u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).
Immunohistochemistry-Frozen: ADAM10 Antibody - BSA Free [NBP1-76973]

Immunohistochemistry-Frozen: ADAM10 Antibody - BSA Free [NBP1-76973]

Immunohistochemistry-Frozen: ADAM10 Antibody - BSA Free [NBP1-76973] - ADAM10 (green) stained human skin, counterstained with DAPI. Tissue fixed, permeablized, and blocked with 5% BSA TBS Ca/Azide. Anti-ADAM10 antibody applied at 1:100, before washed and stained with Alexa488-conjugated secondary. Slides mounted with DAPI. Image from verified customer review.
ADAM10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] -

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 8 Immunofluorescence Validation of ADAM10 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
ADAM10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] -

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 9 Immunofluorescence Validation of ADAM10 in Mouse Testis
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
ADAM10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] -

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 10 Immunofluorescence Validation of ADAM10 in Rat Testis
Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973]

Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973]

Immunocytochemistry/Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - K562 cells with ADAM10 antibody at 10 ug/mL.
ADAM10 Antibody - BSA Free

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] -

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Human Cell Lines.Loading: 15 u of lysate Antibodies: ADAM10, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).
ADAM10 Antibody - BSA Free

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] -

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Rat Tissues.Loading: 15 u of lysates per lane.Antibodies: ADAM10, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).
ADAM10 Antibody - BSA Free

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] -

Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Mouse Tissues.Antibodies: ADAM10, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).

Applications for ADAM10 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 ug/ml

Western Blot

1 ug/mL
Application Notes
IHC- Frozen is reported in verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-76973 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAM10

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Long Name

A Disintegrin and Metalloprotease-like Domain 10

Alternate Names

CD156c, Kuzbanian, MADM

Gene Symbol

ADAM10

UniProt

NP_001101 (Human)

Additional ADAM10 Products

Product Documents for ADAM10 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ADAM10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ADAM10 Antibody - BSA Free

Customer Reviews for ADAM10 Antibody - BSA Free (1)

5 out of 5
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Customer Images

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  • ADAM10 Antibody
    Name: Tyler Crowe
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen human skin sections
    Species: Human
    Verified Customer | Posted 03/08/2019
    ADAM10 (green) stained human skin, counterstained with DAPI.
    Tissue fixed, permeablized, and blocked with 5% BSA TBS Ca/Azide. Anti-ADAM10 antibody applied at 1:100, before washed and stained with Alexa488-conjugated secondary. Slides mounted with DAPI.
    ADAM10 Antibody - BSA Free NBP1-76973

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