ADNP Antibody - BSA Free

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human prostate shows strong nuclear positivity in glandular cells.

Western Blot: ADNP Antibody [NBP1-89236]

Western Blot: ADNP Antibody [NBP1-89236] - Analysis in human cell line SH-SY5Y.

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236]

Immunohistochemistry-Paraffin: ADNP Antibody [NBP1-89236] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot Shows ADNP Specificity Using Knockdown Cell Line.

Western blot shows lysates of DMS 53 parental cell line and ADNP knockdown DMS 53 cell line (KD). Nitrocellulose membrane was probed with ADNP Antibody (Catalog # NBP1-89236) followed by HRP-conjugated secondary antibody. A specific band was detected for ADNP at approximately 135 kDa (as indicated) in the parental DMS 53 cell line, but is significantly reduced in knockdown DMS 53 cell line. Primary antibody concentration used: 0.4 μg/ml. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of ADNP by Immunoprecipitation.

DMS 53 cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of ADNP Antibody (Catalog # NBP1-89236) pre-coupled to Dynabeads Protein A. Immunoprecipitated ADNP was detected in Western Blot with a Rabbit ADNP antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Western Blot: ADNP Antibody - BSA Free [NBP1-89236] -

Expression of ADNP in SIRC cells exposed to UV-B radiations. (A) Representative immunoblots of ADNP expression in SIRC cells following exposure to UV-B insult. The bar graph shows quantitative analysis of signals obtained by immunoblots resulting from three independent experiments. Relative band densities were quantified using ImageJ software. Protein levels are expressed as arbitrary units obtained following normalization to b-tubulin, which was used as loading control. Data represent means +/- SEM. *** p < 0.001 vs. CTRL, as determined by unpaired two-tailed Student t-test. (B) Representative photomicrographs showing ADNP expression (green) in SIRC cells exposed to UV-B radiations. Nuclei were stained with DAPI. Photomicrographs are representative results of fields taken randomly from each slide and scanned by confocal laser scanning microscopy (CLSM; Zeiss LSM700). In the right column, a thin section (0.3 um) with some SIRC cells showing ADNP in the euchromatic compartment of the nuclei is shown. Scale bar in the left and middle columns is 20 μm, and in the right column is 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35052632), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: ADNP Antibody - BSA Free [NBP1-89236] -

Expression of ADNP and p63 in corneal epithelium. H&E staining and immunodetection of ADNP and p63 in human (A) and rabbit (B) cornea: (1) Epithelium, (2) Bowman’s membrane, (3) stroma. Original magnification ×200. Digital micrographs are representative results of fields taken in randomly selected slides and obtained using the Zeiss Axioplan light microscope (Carl Zeiss) fitted with a digital camera (AxioCam MRc5; Carl Zeiss); scale bar: 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35052632), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: ADNP Antibody - BSA Free [NBP1-89236] -

Expression of ADNP and p63 in corneal epithelium. H&E staining and immunodetection of ADNP and p63 in human (A) and rabbit (B) cornea: (1) Epithelium, (2) Bowman’s membrane, (3) stroma. Original magnification ×200. Digital micrographs are representative results of fields taken in randomly selected slides and obtained using the Zeiss Axioplan light microscope (Carl Zeiss) fitted with a digital camera (AxioCam MRc5; Carl Zeiss); scale bar: 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35052632), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: ADNP Antibody - BSA Free [NBP1-89236] -

Expression of ADNP in SIRC cells exposed to UV-B radiations. (A) Representative immunoblots of ADNP expression in SIRC cells following exposure to UV-B insult. The bar graph shows quantitative analysis of signals obtained by immunoblots resulting from three independent experiments. Relative band densities were quantified using ImageJ software. Protein levels are expressed as arbitrary units obtained following normalization to b-tubulin, which was used as loading control. Data represent means +/- SEM. *** p < 0.001 vs. CTRL, as determined by unpaired two-tailed Student t-test. (B) Representative photomicrographs showing ADNP expression (green) in SIRC cells exposed to UV-B radiations. Nuclei were stained with DAPI. Photomicrographs are representative results of fields taken randomly from each slide and scanned by confocal laser scanning microscopy (CLSM; Zeiss LSM700). In the right column, a thin section (0.3 um) with some SIRC cells showing ADNP in the euchromatic compartment of the nuclei is shown. Scale bar in the left and middle columns is 20 μm, and in the right column is 10 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35052632), licensed under a CC-BY license. Not internally tested by Novus Biologicals.