Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Albumin Primary Antibodies »

Product Specifications

Immunogen

Full length protein (Mouse)

Specificity

By immunoelectrophoresis, the antiserum reacts specifically with mouse Albumin. No reactivity was detected against other mouse serum proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat Albumin Antibody - BSA Free (NB600-41532) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Albumin Antibody: Cited in 43 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Albumin Antibody - BSA Free

Western Blot: Albumin AntibodyBSA Free [NB600-41532]

Western Blot: Albumin AntibodyBSA Free [NB600-41532]

Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NB600-41532]

Immunocytochemistry/ Immunofluorescence: Albumin Antibody - BSA Free [NB600-41532]

Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg
Albumin Antibody - BSA Free

Western Blot: Albumin Antibody - BSA Free [NB600-41532] -

Western Blot: Albumin Antibody - BSA Free [NB600-41532] - Primary cell characterization.As per the materials & methods, tumors were isolated from mice & cultured. A, Primary murine hepatocytes were used as controls (Mu). Western blot revealed the primary cultures of APN KO A51, A48, A50 & A52 & WT C53, C54 & C55 cells to express p-mTOR, mTOR, p-Src, Src, PCNA, albumin & AFP. B, Immunofluorescence for K8 & pan-cytokeratin (PK) confirmed that A52 cells were epithelial (Scale bar 20 μM). C, Table showing in vitro & in vivo growth characteristics of WT & APN KO primary cultures. Scale of growth (+ to ++++). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30794695), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Albumin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:30000
Application Notes
Use in IHC-Fr reported in scientific literature (PMID: 33277474). In ELISA, for coating plates use antibody at 1:100 - 1:500. Albumin antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 2 using NB600-41532 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Albumin

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

ALB

Entrez Gene IDs

11657 (Mouse)

Gene Symbol

ALB

UniProt

P07724 (Mouse)

Additional Albumin Products

Product Documents for Albumin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Albumin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Phase Proteins

Citations for Albumin Antibody - BSA Free

Customer Reviews for Albumin Antibody - BSA Free (1)

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  • Albumin Antibody - BSA Free
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 08/05/2022
    Gray Podocalyxin Green Albumin 1:200
    Albumin Antibody - BSA Free NB600-41532
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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