Albumin Antibody

Novus Biologicals | Catalog # NBP1-32458

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline

Cited:

Human, Mouse, Rat

Predicted:

Rhesus Macaque (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, In vitro assay

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Albumin. The exact sequence is proprietary.

Reactivity Notes

Cat (100%), Rabbit (82%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Albumin Antibody

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458]

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458]

Immunocytochemistry/Immunofluorescence: Albumin Antibody [NBP1-32458] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Albumin protein stained by Albumin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Mouse liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Human hepatocellular carcinoma. Albumin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Frozen: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Frozen: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Frozen: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Frozen section of rat liver. Albumin antibody diluted at 1:200.
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Rat liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Albumin Antibody

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] -

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Albumin Antibody

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] -

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog kidney.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Albumin Antibody

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] -

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Cat liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Albumin Antibody

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] -

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Immunofluorescence localization of TNF-alpha, IL-1 beta & IL-6 in the liver tissue of animals treated with ConA or ConA + hVNS. a–c Representative immunofluorescence images of TNF-alpha, IL-1 beta, & IL-6 that were merged with CD11b. Signal intensities of TNF-alpha, IL-1 beta, & IL-6 from 4 to 6 images were averaged per each animal & compared among experimental groups (n = 3 independent experiments). One-way ANOVA with Tukey post hoc is shown. *p < 0.05, **p < 0.01, ***p < 0.001. d Enlarged views of TNF-alpha, IL-1 beta, & IL-6 signals merged with CD11b & Hoechst nuclear staining images in the liver sections from ConA + hVNS animal. e Immunofluorescence staining of liver tissue with anti-CD11b & anti-albumin antibodies. Representative immunofluorescence images using normal mouse IgG antibody instead of primary antibodies followed by reaction with fluorescein-goat anti-mouse IgG antibody are shown in the last column. Scale bars in a–c, d, & e are 100 μm, 10 μm, & 50 μm respectively Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33272194), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Albumin Antibody

Western Blot: Albumin Antibody [NBP1-32458] -

Western Blot: Albumin Antibody [NBP1-32458] - PG-4 whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Albumin Antibody

Western Blot: Albumin Antibody [NBP1-32458] -

Western Blot: Albumin Antibody [NBP1-32458] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Albumin Antibody

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] -

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (NBP1-32458) diluted at 1:500.
Blue: Hoechst 33342 staining.
Albumin Antibody

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] -

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (NBP1-32458) diluted at 1:500.
Blue: Hoechst 33342 staining.
Albumin Antibody

Western Blot: Albumin Antibody [NBP1-32458] -

Western Blot: Albumin Antibody [NBP1-32458] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Albumin Antibody

Western Blot: Albumin Antibody [NBP1-32458] -

Western Blot: Albumin Antibody [NBP1-32458] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Albumin Antibody

Western Blot: Albumin Antibody [NBP1-32458] -

Human plasma (1 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Albumin Antibody

Western Blot: Albumin Antibody [NBP1-32458] -

PD-MSCs combined with WKYMVm enhance hepatic regeneration in the BDL rat model. mRNA expression of HNF1 alpha (A), ALB (B), FPR2 (C), and HGFR (D) in BDL rat liver shown by qRT-PCR. (E) Protein levels of ALB, cyclin D1, gp130, IL-6, p/tSTAT3, and GAPDH in BDL rat livers shown by Western blots. (F) Expression of PCNA in BDL rat liver shown by immunohistochemistry. Scale bar = 25 μm. (G) Quantification of the PCNA-positive area in BDL rat livers through immunohistochemistry. (H) Secreted HGF level in BDL rat serum shown by ELISAs. n = 3 rats per group, mean +/- SD, * p < 0.05 by one-way ANOVA. Con, control; NTx, nontransplantation group; Tx, PD-MSC transplantation group; Tx+WK, PD-MSCs with WKYMVm combined transplantation group; wk, week. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35053347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Albumin Antibody

Western Blot: Albumin Antibody [NBP1-32458] -

PD-MSCs combined with WKYMVm can regenerate damaged hepatocytes in vitro. (A) Schematic figure of WB-F344s cell in vitro modeling. (B) Cell viability of the LCA-treated WB-F344s cells shown by CCK-8 assays. Protein expression of ALB (C) and HNF1 alpha (D) shown using Western blots. Protein expression was normalized to GAPDH expression through Western blot bands. (E) Localization and expression of translocated HNF1 alpha in the nucleus shown through immunofluorescence. (F) Quantification of translocated HNF1 alpha -positive WB-F344s versus total WB-F344s using immunofluorescence. n = 3 per group, mean +/- SD, * p < 0.05 by t tests. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35053347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Albumin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

In vitro assay

Assay dependent

Western Blot

1:500-1:100000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Albumin

Albumin is a soluble, monomeric protein which comprises about one half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Alternate Names

ALB

Gene Symbol

ALB

Additional Albumin Products

Product Documents for Albumin Antibody

Certificate of Analysis

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Product Specific Notices for Albumin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Albumin Antibody

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Protocols

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