Albumin Antibody
Novus Biologicals | Catalog # NBP1-32458
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Feline
Cited:
Human, Mouse, Rat
Predicted:
Rhesus Macaque (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, In vitro assay
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Albumin. The exact sequence is proprietary.
Reactivity Notes
Cat (100%), Rabbit (82%).
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Albumin Antibody
Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458]
Immunocytochemistry/Immunofluorescence: Albumin Antibody [NBP1-32458] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Albumin protein stained by Albumin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Mouse liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Human hepatocellular carcinoma. Albumin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Immunohistochemistry-Frozen: Albumin Antibody [NBP1-32458]
Immunohistochemistry-Frozen: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Frozen section of rat liver. Albumin antibody diluted at 1:200.Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458]
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Rat liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] -
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] -
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog kidney.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] -
Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Cat liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] -
Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Immunofluorescence localization of TNF-alpha, IL-1 beta & IL-6 in the liver tissue of animals treated with ConA or ConA + hVNS. a–c Representative immunofluorescence images of TNF-alpha, IL-1 beta, & IL-6 that were merged with CD11b. Signal intensities of TNF-alpha, IL-1 beta, & IL-6 from 4 to 6 images were averaged per each animal & compared among experimental groups (n = 3 independent experiments). One-way ANOVA with Tukey post hoc is shown. *p < 0.05, **p < 0.01, ***p < 0.001. d Enlarged views of TNF-alpha, IL-1 beta, & IL-6 signals merged with CD11b & Hoechst nuclear staining images in the liver sections from ConA + hVNS animal. e Immunofluorescence staining of liver tissue with anti-CD11b & anti-albumin antibodies. Representative immunofluorescence images using normal mouse IgG antibody instead of primary antibodies followed by reaction with fluorescein-goat anti-mouse IgG antibody are shown in the last column. Scale bars in a–c, d, & e are 100 μm, 10 μm, & 50 μm respectively Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33272194), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Albumin Antibody [NBP1-32458] -
Western Blot: Albumin Antibody [NBP1-32458] - PG-4 whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Albumin Antibody [NBP1-32458] -
Western Blot: Albumin Antibody [NBP1-32458] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] -
Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (NBP1-32458) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] -
Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Albumin stained by Albumin antibody (NBP1-32458) diluted at 1:500.
Blue: Hoechst 33342 staining.
Western Blot: Albumin Antibody [NBP1-32458] -
Western Blot: Albumin Antibody [NBP1-32458] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Albumin Antibody [NBP1-32458] -
Western Blot: Albumin Antibody [NBP1-32458] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Western Blot: Albumin Antibody [NBP1-32458] -
Human plasma (1 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Albumin Antibody [NBP1-32458] -
PD-MSCs combined with WKYMVm enhance hepatic regeneration in the BDL rat model. mRNA expression of HNF1 alpha (A), ALB (B), FPR2 (C), and HGFR (D) in BDL rat liver shown by qRT-PCR. (E) Protein levels of ALB, cyclin D1, gp130, IL-6, p/tSTAT3, and GAPDH in BDL rat livers shown by Western blots. (F) Expression of PCNA in BDL rat liver shown by immunohistochemistry. Scale bar = 25 μm. (G) Quantification of the PCNA-positive area in BDL rat livers through immunohistochemistry. (H) Secreted HGF level in BDL rat serum shown by ELISAs. n = 3 rats per group, mean +/- SD, * p < 0.05 by one-way ANOVA. Con, control; NTx, nontransplantation group; Tx, PD-MSC transplantation group; Tx+WK, PD-MSCs with WKYMVm combined transplantation group; wk, week. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35053347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: Albumin Antibody [NBP1-32458] -
PD-MSCs combined with WKYMVm can regenerate damaged hepatocytes in vitro. (A) Schematic figure of WB-F344s cell in vitro modeling. (B) Cell viability of the LCA-treated WB-F344s cells shown by CCK-8 assays. Protein expression of ALB (C) and HNF1 alpha (D) shown using Western blots. Protein expression was normalized to GAPDH expression through Western blot bands. (E) Localization and expression of translocated HNF1 alpha in the nucleus shown through immunofluorescence. (F) Quantification of translocated HNF1 alpha -positive WB-F344s versus total WB-F344s using immunofluorescence. n = 3 per group, mean +/- SD, * p < 0.05 by t tests. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35053347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Albumin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
In vitro assay
Assay dependent
Western Blot
1:500-1:100000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Albumin
Alternate Names
ALB
Gene Symbol
ALB
Additional Albumin Products
Product Documents for Albumin Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Albumin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Albumin Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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