Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Novus Biologicals | Catalog # NB100-2563

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RELGEYGFHEYTE corresponding to internal region according to NP_000680.2.

Marker

Astrocyte Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - HEK293 overexpressing ALDH1A1 and probed with NB100-2563 (mock transfection in first lane).
Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563]

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563]

Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563]

Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NB100-2563] - (1ug/ml) Analysis of Human Liver Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 55 kDa band observed in human liver lysates (calculated MW of 54.9 kDa band according to NP_000680.2).
IHC: Paraffin embedded Human Testis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1

This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes.

Alternate Names

ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1

Entrez Gene IDs

216 (Human)

Gene Symbol

ALDH1A1

UniProt

P00352

Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products

Product Documents for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

Certificate of Analysis

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Product Specific Notices for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody

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Protocols

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