ALDH1A3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15339

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Simple Western, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aldehyde Dehydrogenase 1-A3/ALDH1A3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34572374). Mouse reactivity reported in scientific literature (PMID: 28249000). Chicken (88%)., Xenopus laevis (83%)..

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ALDH1A3 Antibody - BSA Free

Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Total protein from human Lymph node, mouse Stomach and A431 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ALDH1A3 (NBP2-15339) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-ALDH1A3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry: ALDH1A3 Antibody - BSA Free [NBP2-15339]

ALDH1A3-Antibody---BSA-Free-Immunohistochemistry-NBP2-15339-img0020.jpg
ALDH1A3 Antibody - BSA Free-Western Blot-NBP2-15339-img0023.jpg
Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 AntibodyBSA Free [NBP2-15339]

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ALDH1A3 antibody [N2C2], diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Blue: Hoechst 33342 staining.
Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunocytochemistry/Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T. Human breast cancer cells were transduced with human Aldh1a3 and probed with antibody at 1:500 overnight at 4C followed by counterstaining with secondary and DAPI. Strong signal observed compared to knockout controls. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Frozen: ALDH1A3 Antibody - BSA Free [NBP2-15339] - 8-week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with ALDH1A3 (1:500 dilution) and Insulin (1:1000 dilution) antibodies. Blue arrows indicate insulin+Aldh1A3+ cells. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Mouse prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339]

Immunohistochemistry-Paraffin: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Rat prostate. ALDH1A3 stained by ALDH1A3 antibody [N2C2], diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
ALDH1A3 Antibody - BSA Free

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] -

Western Blot: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Palbociclib induces a potential transition of PN GSCs to MES GSCs(A) qPCR analysis of markers of PN & MES subtypes in G44 & G559 treated with the indicated doses of palbociclib for 5 days. n = 3. Differences were analyzed between the control & the palbociclib-treated groups. NS: not statistically significant; #p < 0.05; *p < 0.01; **p < 0.001. (B) qPCR & immunoblot analysis of ALDH1A3 in three independent PN GSC lines that were treated with 10 nM of palbociclib for 5 days. n = 3. Differences were analyzed between the control & the palbociclib-treated groups. NS: not statistically significant; #p < 0.05; *p < 0.01; **p < 0.001. (C) Cell proliferation analysis of three independent PN GSC lines that were treated with palbociclib (10 nM), DEAB (25 μM), or both for 5 days. The cells were cultured on a laminin (10 μg/ml in poly ornithine)-coated 96-well plate. Cell number was determined by CyQUANT Direct Cell Proliferation assay. n = 3. *p < 0.05, comparison between palbociclib or DEAB treatment with the vehicle control; #p < 0.05, comparison between combination group with palbociclib treatment. G44, CDI = 0.79; G559, CDI = 0.565; G816, CDI = 0.677. Shown are representative data of three independent experiments with similar results. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.19429), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ALDH1A3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] -

Immunocytochemistry/ Immunofluorescence: ALDH1A3 Antibody - BSA Free [NBP2-15339] - Markers of dedifferentiation are present in islets of db/db, but not weight-matched WD-fed mice. (a) Immunofluorescent analysis of islets from weight-matched WD-fed mice (top row) & db/db mice (bottom row) showing Nkx6.1 (red), insulin (green), & DAPI (blue). In the merged image, note the loss of double-positive nuclei (DAPI plus Nkx6.1; purple color) in the db/db mice but not in the WD-fed mice. (b) Expression of the Aldh1a3 gene in islets isolated from mice fed a WD for 4 or 12 weeks normalized to mice fed a control diet compared with db/db mice at 8 weeks of age (normalized to lean db/+ controls). ∗∗∗p < 0.001 versus both WD groups by one-way ANOVA. (c) Staining for Aldh1a3 protein (red) & insulin (green) in islets from weight-matched WD-fed mice (20 weeks on diet) versus db/db mice (14 weeks of age). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29038790), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ALDH1A3 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 27572106)

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000. Use reported by customer review

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Frozen

reported by customer review and in scientific literature (PMID 26713822)

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000

Reviewed Applications

Read 3 reviews rated 4.7 using NBP2-15339 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aldehyde Dehydrogenase 1-A3/ALDH1A3

Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form. [provided by RefSeq]

Long Name

Aldehyde Dehydrogenase 1 Family Member A3

Alternate Names

ALDH1A3, ALDH6, MCOP8, RALDH3

Gene Symbol

ALDH1A3

UniProt

P47895 (Human)

Additional Aldehyde Dehydrogenase 1-A3/ALDH1A3 Products

Product Documents for ALDH1A3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ALDH1A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adult Neurogenesis
Redox Enzymes

Citations for ALDH1A3 Antibody - BSA Free

Customer Reviews for ALDH1A3 Antibody - BSA Free (3)

4.7 out of 5
3 Customer Ratings
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • ALDH1A3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: T47D human breast cancer cell line
    Species: Human
    Verified Customer | Posted 03/08/2020
    Left 6 lanes, empty vector and overexpression vectors of ALDH1A3 in T47D cell lines Right 6 lanes, empty vector and overexpression vectors of ALDH1A3 in MDA-MB-468 cell lines
    ALDH1A3 Antibody - BSA Free NBP2-15339
  • ALDH1A3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human breast cancer cells
    Species: Human
    Verified Customer | Posted 01/14/2020
    Human breast cancer cells were transduced with human Aldh1a3 and then were probed with antibody at 1:500 overnight at 4C followed by counterstaining with secondary and DAPI. Strong signal observed compared to knockout controls
    Human breast cancer cells with Aldh1a3 overexpression were stained at 1:500 in 5% goat serum in PBS-T.
    ALDH1A3 Antibody - BSA Free NBP2-15339
  • ALDH1A3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Mouse Pancreas
    Species: Mouse
    Verified Customer | Posted 11/09/2016
    8 Week old mouse pancreas cryosections with beta cell dedifferentiation properties were stained with Aldh1A3 (1:500), Insulin (1:1000). Blue arrows indicate insulin+Aldh1A3+ cells.
    ALDH1A3 Antibody - BSA Free NBP2-15339

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