Alix Antibody - BSA Free

Flow Cytometry: Alix Antibody - BSA Free [NBP1-49701]

Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on NIH3T3 cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/ Immunofluorescence: Alix Antibody - BSA Free [NBP1-49701]

Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-49701] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Alix Antibody (NBP1-49701) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: Alix Antibody - BSA Free [NBP1-49701]

Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on HeLa cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Simple Western: Alix AntibodyBSA Free [NBP1-49701]

Simple Western: Alix Antibody - BSA Free [NBP1-49701] - Alix Antibody [NBP1-49701] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Alix antibody (NBP1-49701) at approximately 106 kDa (as indicated) using 1:10 of Alix antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western: Alix Antibody - BSA Free [NBP1-49701] -

Patient derived NK cells can be expanded in vitro and NKEVs contain protein and nucleic acid cargo reflecting the cells of origin. NK cells were isolated from patient PBMCs via negative selection and EVs were isolated with size exclusion chromatography from the NK cell culture medium (n=20, 10 �pre� and 10 �post) (a). Representative image of cytokine profiling (n=12) of the secretome showed the presence of classic chemokines (CCL1, -5) and cytokines (IFNy, GM-CSF) (b). NKEVs were 100�200 nm in diameter post SEC (n=20) (c) and express canonical EV markers and cytotoxic NK proteins (d). Whole transcriptome sequencing (n=21; 20 patient NKEV and 1 control pool NKEV) found the majority of NKEV RNA cargo is protein coding and long noncoding transcripts (e). Differential gene expression (DE) analysis of LUAD/LUSC NKEVs identified a small number of significantly DE transcripts between LUAD and LUSC, such as ERAP2, which is upregulated in LUAD NKEVs (f). Mass spectrometry proteomics analysis (n=21; 20 patient NKEV and 1 control pool NKEV) of NKEV cargo identified over 4000 proteins (g), and modestly differentially expressed proteins between LUAD and LUSC groups (h). (b) is one representative dot blot, PT007-post and (d) is one representative Western blot, PT007-post. (c) shows NTA analysis for all individual samples in grey, with the mean distribution in red and SEM error bars. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/40821787/), licensed under a CC-BY license. Not internally tested by Novus Biologicals.