Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80568
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Radioimmunodiffusion
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 8B6
Format
Azide and BSA Free
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Product Specifications
Immunogen
Hep-2 cells with boosted surface expression of Alkaline Phosphatase, Placental. [UniProt# P05187].
Reactivity Notes
Not yet tested in other species. Mouse reactivity reported in scientific literature (PMID: 28197547). Rat reactivity reported in scientific literature (PMID: 30599898).
Localization
Cell membrane; Lipid-anchor > GPI-anchor
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
![Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6)Azide and BSA Free [NBP2-80568]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Western-Blot-NBP2-80568-img0001.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6)Azide and BSA Free [NBP2-80568]")
Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6)Azide and BSA Free [NBP2-80568]
Western Blot: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80568-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]")
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]
Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568] - analysis of ALPP in MDA-MB-231 cells using an anti-ALPP antibody (blue - cell membrane, green - ALPP). Image from verified customer review. Image from the standard format of this antibody.![Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80568-img0003.jpg "Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]")
Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]
Immunohistochemistry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568] - IHC staining of Alkaline Phosphatase (Placental) in human placenta using DAB with hematoxylin counterstain. Proteolytic Induced Epitope Retrieval (PIER) was used. Image from the standard format of this antibody.![Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]](https://resources.rndsystems.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-8B6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80568-img0004.jpg "Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]")
Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568]
Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free [NBP2-80568] - Detection of Alkaline Phosphatase/ALPP in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Alkaline Phosphatase/ALPP Monoclonal Antibody (Catalog # NB110-3638, filled histogram), or Mouse IgG2A isotype control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a Bio-Techne brand. Image from the standard format of this antibody.Applications for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
reported in multiple pieces of scientific literature
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:100-1:200
Western Blot
1:1000
Application Notes
In WB a band can be seen at ~70 kDa. For IHC, Proteolytic Induced Epitope Retrieval (PIER) is required. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Alkaline Phosphatase/ALPP Antibody (8B6) - Azide and BSA Free
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Q: I am looking to use a placental alkaline phosphatase antibody for sorting syncytiotrophoblast material from female tissue. It looks like most people who use these antibodies use in-house versions and references for commercially available antibodies I've found are from the '80's. I noticed you have several different monoclonal antibodies and was hoping you could give me a little more information on them and potential applications they've been tested for. Also, do you have a biotinylated or fluorophore-conjugated version?
A:
None of our placental alkaline phosphatase antibodies are biotinylated or fluorchrome conjugated but we offer a number of kits for this application and our lab can do a custom conjugation as well, for an additional fee. NB600-750 is useful in FACS with human cells, so I would recommend this antibody for your experiment. We back the use of this antibody as stated on the datasheet with the 100% Novus Guarantee. If you have questions about another antibody, I will be happy to answer them.
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