alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

Novus Biologicals | Catalog # H00000174-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G7

Format

Azide and BSA Free
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

AFP (AAH27881, 500 a.a. ~ 609 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV

Specificity

AFP - alpha-fetoprotein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody (M01), clone 1G7.Lane 1: AFP transfected lysate(69 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunofluorescence of monoclonal antibody to alpha-Fetoprotein/AFP on HepG2 cell. [antibody concentration 30 ug/ml]
Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunoperoxidase of monoclonal antibody to AFP ( Cat # H00000174-M01 ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - AFP monoclonal antibody (M01), clone 1G7 Analysis of AFP expression in HepG2.
Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunocytochemistry/ Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of monoclonal antibody to AFP on HepG2 cell. Antibody concentration 30 ug/ml.
Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP transfected lysate using anti-AFP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AFP MaxPab rabbit polyclonal antibody.
ELISA: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

ELISA: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01]

ELISA: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Detection limit for recombinant GST tagged AFP is approximately 0.03ng/ml as a capture antibody.

Applications for alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-Fetoprotein/AFP

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human)

Gene Symbol

AFP

OMIM

104150 (Human)

UniProt

AAH27881 (Human)

Additional alpha-Fetoprotein/AFP Products

Product Documents for alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Fetoprotein/AFP Antibody (1G7) - Azide and BSA Free

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Protocols

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FAQs

No product specific FAQs exist for this product.

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