Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 132D3

Format

BSA Free
View all alpha-Smooth Muscle Actin Primary Antibodies »

Product Specifications

Immunogen

Alpha-Smooth Muscle Actin Antibody (132D3) is made to a recombinant human ACTA2 (3-377aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free (NBP2-59429) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free

Western Blot: alpha-Smooth Muscle Actin Antibody (132D3)BSA Free [NBP2-59429]

Western Blot: alpha-Smooth Muscle Actin Antibody (132D3)BSA Free [NBP2-59429]

Western Blot: alpha-Smooth Muscle Actin Antibody (132D3) [NBP2-59429] - Western blot analysis of the following samples blotted with alpha-Smooth Muscle Actin Antibody (132D3): Lane 1: HepG2 cell lysates, Lane 2: SW480 cell lysates, Lane 3: HeLa cell lysates. Bands indicate an observed molecular weight of ~42 kDa.
Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free [NBP2-59429]

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free [NBP2-59429]

Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (132D3) [NBP2-59429] - Analysis of alpha-Smooth Muscle Actin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal alpha-Smooth Muscle Actin Antibody (132D3) (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Applications for alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free

Application
Recommended Usage

Western Blot

1:1000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free

Certificate of Analysis

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Product Specific Notices for alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for alpha-Smooth Muscle Actin Antibody (132D3) - BSA Free

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    • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
      A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.
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