alpha-Smooth Muscle Actin Antibody (1H8)

Novus Biologicals | Catalog # NBP2-37641

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H8
View all alpha-Smooth Muscle Actin Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human alpha-Smooth Muscle Actin expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody (1H8)

Western Blot: alpha-Smooth Muscle Actin Antibody (1H8)BSA Free [NBP2-37641]

Western Blot: alpha-Smooth Muscle Actin Antibody (1H8)BSA Free [NBP2-37641]

Western Blot: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis using alpha-Smooth Muscle Actin Antibody (1H8) against Hela (1), Jurkat (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate. Bands indicate an observed molecular weight of ~42 kDa.
Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Immunocytochemistry/Immunofluorescence: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

Flow Cytometry: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Analysis of Hela cells using alpha-Smooth Muscle Actin Antibody (1H8) (green) and negative control (purple).
ELISA: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

ELISA: alpha-Smooth Muscle Actin Antibody (1H8) - BSA Free [NBP2-37641]

ELISA: alpha-Smooth Muscle Actin Antibody (1H8) [NBP2-37641] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for alpha-Smooth Muscle Actin Antibody (1H8)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2.El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G.,... Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M.,... Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody (1H8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for alpha-Smooth Muscle Actin Antibody (1H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for alpha-Smooth Muscle Actin Antibody (1H8)

Customer Reviews for alpha-Smooth Muscle Actin Antibody (1H8)

There are currently no reviews for this product. Be the first to review alpha-Smooth Muscle Actin Antibody (1H8) and earn rewards!

Have you used alpha-Smooth Muscle Actin Antibody (1H8)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for alpha-Smooth Muscle Actin Antibody (1H8)

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?

    A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies