Q: I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?

A: We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

alpha-Smooth Muscle Actin Antibody (SPM332) [Allophycocyanin]

Novus Biologicals | Catalog # NBP2-34760APC

(1)

Citations (3)

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Baboon, Bovine, Canine, Chicken, Feline, Goat, Guinea Pig, Monkey, Rabbit, Sheep

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPM332

View all alpha-Smooth Muscle Actin Primary Antibodies »

Product Specifications

Immunogen

N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH. (Uniprot: P62736)

Localization

Cytoplasmic

Marker

Leiomyosarcoma Marker

Specificity

Actin is a major component of the cytoskeleton and is present in most cell types. This monoclonal antibody is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This monoclonal antibody does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody (SPM332) [Allophycocyanin]

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Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [Allophycocyanin] [NBP2-34760APC] -

Cells expressing IL-1⍺ & IL-1R are observed in the fibrotic liver environment. (a) Cytokines in tissue lysates from mice at 9 wpi were measured by ELISA in liver. Data are presented as fold change relative to the mean of uninfected levels. N = 11–12 mice per group, pooled from three independent experiments. (b) IL-1 alpha levels in the sera at 9 wpi measured by ELISA. N = 9–14 mice per group, pooled from four independent experiments. (c) Immunofluorescence labeling of nuclei (DAPI white), IL-1⍺ (green), CD45 (red), & collagen1⍺1 (blue) in the liver of UI or 9 wpi WT mice. Number of cells staining positive for CD45 and/or IL-1⍺, average 2–3 fields of view where immune infiltrate was present from 3 mice per condition are quantified on the right. Error bars are standard deviation. (d) Immunofluorescent labeling of nuclei (DAPI white), ⍺-smooth muscle actin (green), IL-1R (red), & collagen1⍺ (blue) in the liver of uninfected or 9 wpi WT. Inset, arrow head represents ⍺-smooth muscle actin/IL-1R co-staining cells (arrow heads). Number of cells staining positive for IL-1R and/or ⍺-SMA, average of 4–8 fields of view from 3 mice are quantified on the right. Error bars are standard deviation. (c,d) represent maximum intensity projections of 9–13 μm thick z-stacks. Scale bar represents 50 μm. Error bars are standard error of the mean except where noted otherwise. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired Student’s t test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32973293), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [Allophycocyanin] [NBP2-34760APC] -

Immunocytochemistry/ Immunofluorescence: alpha-Smooth Muscle Actin Antibody (SPM332) [Allophycocyanin] [NBP2-34760APC] - IL-1 induces contractility & alpha smooth muscle actin expression in murine embryonic fibroblasts & primary hepatic stellate cells. (a–c) MEFs were incubated with media, 10 ng/mL IL-1 alpha, 150 pg/mL IL-1 beta or 10 ng/mL TGF beta -1 for 48 h. After fixation, MEFs were stained for F-actin, & alpha-smooth muscle actin ( alpha -SMA). Cell spreading was quantified in (b), & levels of alpha -SMA expression were quantified in (c) as the mean relative to untreated for each biological replicate (left panels) or the single cell data pooled from three biological replicate experiments (right panels). For each experiment, 50–200 cells/group were analyzed. (d–f) Primary hepatic stellate cells (HSCs) were isolated from uninfected mouse livers, & FACS-sorted based on endogenous retinoid fluorescence (d). (e,f) HSCs were seeded onto 4 kPa hydrogels coated with 10 μg/mL of fibronectin & cultured with 10 ng/mL IL-1 alpha, 10 ng/mL TGF-beta, or media alone for 48 h & then fixed & stained for F-actin & alpha -SMA & imaged by confocal microscopy. Scale bar represents 50 μm. Total cell area quantified in (e) & levels of alpha -SMA expression were quantified in terms of pixels/cell in (f) as the mean relative to untreated for each biological replicate (left panels) or the single cell data pooled from four biological replicate experiments (right panels). Error bars are standard error of the mean. For the left panels, data were compared by one-way ANOVA with Bonferroni’s multiple comparisons test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32973293), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

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