alpha-Synuclein Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-15365

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (94%), Chimpanzee (100%), Porcine (97%), Sheep (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all alpha-Synuclein Primary Antibodies »

Product Specifications

Immunogen

Full length human alpha-Synuclein Recombinant protein.

Reactivity Notes

Chicken (88%), Xenopus laevis (85%).

Localization

Cytoplasm; Membrane; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit alpha-Synuclein Antibody - BSA Free (NBP2-15365) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-alpha-Synuclein Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for alpha-Synuclein Antibody - Azide Free

Western Blot: alpha-Synuclein Antibody [NBP2-15365]

Western Blot: alpha-Synuclein Antibody [NBP2-15365]

Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365]

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365]

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Rat brain. alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365]

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365]

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Mouse hippocampus. alpha Synuclein stained by alpha Synuclein antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365]

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365]

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - Human colon carcinoma, using alpha Synuclein antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
alpha-Synuclein Antibody

Western Blot: alpha-Synuclein Antibody [NBP2-15365] -

Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha-Synuclein antibody (NBP2-15365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha-Synuclein Antibody

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] -

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-Synuclein Antibody

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] -

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] - alpha-Synuclein antibody detects alpha-Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
alpha-Synuclein stained by alpha-Synuclein antibody (NBP2-15365) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha-Synuclein Antibody - Azide Free

Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] -

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500.
alpha-Synuclein Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: alpha-Synuclein Antibody [NBP2-15365] -

alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.
Blue: Fluoroshield with DAPI.
alpha-Synuclein Antibody - Azide Free

Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] -

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.
Blue: Fluoroshield with DAPI.
alpha-Synuclein Antibody - Azide Free

Western Blot: alpha-Synuclein Antibody [NBP2-15365] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (NBP2-15365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
alpha-Synuclein Antibody - Azide Free

Immunohistochemistry-Frozen: alpha-Synuclein Antibody [NBP2-15365] -

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (NBP2-15365) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
alpha-Synuclein Antibody - Azide Free

Western Blot: alpha-Synuclein Antibody [NBP2-15365] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (NBP2-15365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha-Synuclein Antibody - Azide Free

Western Blot: alpha-Synuclein Antibody [NBP2-15365] -

Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (NBP2-15365) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
alpha-Synuclein Antibody - Azide Free

Immunohistochemistry-Paraffin: alpha-Synuclein Antibody [NBP2-15365] -

alpha Synuclein antibody detects alpha Synuclein protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Green: alpha Synuclein antibody (NBP2-15365) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488).
Blue: Nuclear staining with Hoechst 33342.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for alpha-Synuclein Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Sandwich ELISA

Reported in scientific literature (PMID:35902984).

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-15365 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-Synuclein

Alpha-synuclein, a member of the synuclein family, is a protein that was first identified in 1988 whose name is derived from its localization to both the synapse and nucleus (1-3). Specifically, it is expressed primarily in the brain, including Lewy Bodies (1-6). Alpha-synuclein is encoded by the SNCA gene, located on chromosome 4p21, and is processed as a 140 amino acid (aa) protein with a theoretical molecular weight of 14 kDa (1,2,4). Structurally alpha-synuclein consists of a N-terminal binding domain (1-60 aa), a central domain core region called the non-amyloid-beta component (NAC) (61-95 aa), and a C-terminal domain (96-140 aa) (1-3). The N-terminal region contains aa repeats with a KTKEGV consensus sequence that gives the protein its alpha-helical structure that associates with lipid membranes (1-4). The hydrophobic NAC region is responsible for alpha-synuclein aggregation and fibril formation (1-4). The acidic C-terminal tail is largely unstructured but can be targeted for post-translational modifications (1-4). The function of alpha-synuclein is not entirely understood, but it is shown to have a role in suppression of apoptosis, acting as a molecular chaperone, regulating glucose, and modulating calmodulin activity (1,3).

A number of studies have revealed that alpha-synuclein aggregation is a hallmark feature in a number of neurodegenerative diseases, referred to as synucleinopathies (2-4). Alpha-synuclein protein aggregates are a large component of Lewy bodies that are present in Parkinson's disease (PD), Lewy body dementia (LBD), and multiple system atrophy (1-6). Research has shown phosphorylation of alpha-synuclein at Ser129 moves the protein from the nucleus to the cytoplasm and promotes fibril formation associated with synucleinopathies (1,2,5). Recent studies also suggest that alpha-synuclein accumulation can prevent mitochondrial import machinery causing mitochondrial dysfunction that is often observed in neurodegeneration (5). It is thought that preventing alpha-synuclein aggregation may prevent PD, thus alpha-synuclein is a target for many potential therapeutic interventions aimed at decreasing aggregate formation or increasing clearance (1,2,4-6).

References

1. Villar-Pique, A., Lopes da Fonseca, T., & Outeiro, T. F. (2016). Structure, function and toxicity of alpha-synuclein: the Bermuda triangle in synucleinopathies. Journal of neurochemistry. https://doi.org/10.1111/jnc.13249

2. Emamzadeh F. N. (2016). Alpha-synuclein structure, functions, and interactions. Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences. https://doi.org/10.4103/1735-1995.181989

3. Burre J. (2015). The Synaptic Function of alpha-Synuclein. Journal of Parkinson's disease. https://doi.org/10.3233/JPD-150642

4. Lashuel, H. A., Overk, C. R., Oueslati, A., & Masliah, E. (2013). The many faces of alpha-synuclein: from structure and toxicity to therapeutic target. Nature reviews. Neuroscience. https://doi.org/10.1038/nrn3406

5. Rocha, E. M., De Miranda, B., & Sanders, L. H. (2018). Alpha-synuclein: Pathology, mitochondrial dysfunction and neuroinflammation in Parkinson's disease. Neurobiology of disease. https://doi.org/10.1016/j.nbd.2017.04.004

6. O'Leary, E. I., & Lee, J. C. (2019). Interplay between alpha-synuclein amyloid formation and membrane structure. Biochimica et biophysica acta. Proteins and proteomics. https://doi.org/10.1016/j.bbapap.2018.09.012

Alternate Names

NACP, PARK1, PARK4, SNCA, Synuclein-alpha

Gene Symbol

SNCA

Additional alpha-Synuclein Products

Product Documents for alpha-Synuclein Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for alpha-Synuclein Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Neuronal Lineage Markers

Citations for alpha-Synuclein Antibody - Azide Free

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  • alpha-Synuclein Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse forebrain lysate
    Species: Mouse and Human
    Verified Customer | Posted 12/18/2017
    Alpha-synuclein in forebrain of wildtype (WT) and transgenic (Tg) mice expressing human a-syncuclein
    alpha-Synuclein Antibody - Azide Free NBP2-15365

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FAQs for alpha-Synuclein Antibody - Azide Free

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  • Q: I'm looking for an alpha-Synuclein antibody with an epitope located in the first half (N-terminus) of the protein - preferably a monoclonal antibody. Can you help me with that?

    A: Please take a look at NB110-57475. It has been validated for human, rat and mouse and the applications ICC and WB and the epitope it detects is in the N terminal.

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