alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80571

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Avian, C. elegans, Drosophila, Invertebrate, Mammal, Monkey, Primate, Yeast

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Radioimmunoassay, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # YL1/2

Format

Azide and BSA Free
View all alpha Tubulin Primary Antibodies »

Product Specifications

Immunogen

This alpha Tubulin Antibody (YL1/2) was developed against full length native protein (purified) (S. cerevisiae).

Epitope

A linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).

Reactivity Notes

S. cerevisiae, S. pombe, Slime molds, Allium. Other species have not been tested. Expected to react with most eukaryotes due to sequence identity. Drosophila reactivity reported in scientific literature (PMID: 24019759). C. elegans reactivity reported in scientific literature (PMID: 29118344). Toxoplasma gondii reactivity reported by customer review.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Western Blot analysis of whole cell lysates of HeLa, NIH-3T3 and PC-12 cell lines using Tubulin antibody (clone YL1/2). Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin YL1/2 antibody was tested in HeLa cells with Dylight 550 (red). Nuclei were counterstained with DAPI (blue). Image from the standard format of this antibody.
Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunohistochemistry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tubulin antibody was tested in human breast cancer xenograft using DAB with hematoxylin counterstain. Image from the standard format of this antibody.
Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on SH-SY5Y cells with alpha Tubulin (YL1/2) Antibody NB600-506G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to DyLight 488.
Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Tissue levels of VEGF and PCNA.VEGF levels were estimated by western blot. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0112995), licensed under a CC-BY license. Image from the standard format of this antibody.
Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Total protein from human HeLa, mouse 3T3, rat PC12 and African green monkey Cos7 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with a 1:6000 dilution of anti-Tubulin (YL 1/2) in 1% non-fat milk in TBST and detected with an anti-rat HRP secondary antibody using chemiluminescence. Alpha tubulin molecular weight: 50 kDa. Image from the standard format of this antibody.
Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2)Azide and BSA Free [NBP2-80571]

Western Blot: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Western blot analysis of alpha Tubulin (molecular weight: 50 kDa) expression in 1) HeLa, 2) NTERA-2, 3) A431, 4) HepG2, 5) MCF7, 6) NIH-3T3, 7) PC-12 and 8) Cos 7 whole cell lysates using NB600-506. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - B-Tubulin staining (488) and Pericentrin (594) in Mitotic Cell. Image from verified customer review. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Tubulin (YL1/2) at a 1:200 dilution overnight at 4C and detected with an anti-rat Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - IF Confocal analysis of NIH/3T3 cells using Tubulin antibody (NB600-506, 1:5). An Alexa Fluor 488-conjugated Goat to rat IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from the standard format of this antibody.
Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on NIH3T3 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - An intracellular stain was performed on PC12 cells with Tubulin [YL1/2] Antibody NB600-506AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.
Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571]

Flow Cytometry: alpha Tubulin Antibody (YL1/2) - Azide and BSA Free [NBP2-80571] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-506. An intracellular stain was performed on HeLa cells with Tubulin antibody (YL1/2) NB600-506AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

Applications for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:1000

Flow Cytometry

2-5 ug/0.1x10^6 cells

Immunocytochemistry/ Immunofluorescence

1:1000-1:10000

Immunohistochemistry

1:200

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:5000-1:10000
Application Notes
This Tubulin Antibody (YL1/2) is useful for Western blot, Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Radioimmunoassay and ELISA. NB600-506 is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in Immunocytochemistry.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha Tubulin

Tubulin is the major building block of microtubules. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. The tubulin superfamily is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa.

Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Like GAPDH and beta-actin, alpha/beta tubulin is often used as a loading control in immunoblot applications (1). Alpha/beta tubulin is also good for counterstaining microtubules in immunofluorescence (2).

References

1. Hannen, R., Selmansberger, M., Hauswald, M., Pagenstecher, A., Nist, A., Stiewe, T.,... Bartsch, J. W. (2019). Comparative Transcriptomic Analysis of Temozolomide Resistant Primary GBM Stem-Like Cells and Recurrent GBM Identifies Up-Regulation of the Carbonic Anhydrase CA2 Gene as Resistance Factor. Cancers (Basel), 11(7). doi:10.3390/cancers11070921

2. Nel, M., Joubert, A. M., Dohle, W., Potter, B. V., & Theron, A. E. (2018). Modes of cell death induced by tetrahydroisoquinoline-based analogs in MDA-MB-231 breast and A549 lung cancer cell lines. Drug Des Devel Ther, 12, 1881-1904. doi:10.2147/dddt.S152718

Long Name

Tubulin Alpha 1a

Alternate Names

Alpha-Tubulin 3, B-ALPHA-1, LIS3, TUBA1A, TUBA3, Tubulin B-Alpha-1

Gene Symbol

TUBA1A

Additional alpha Tubulin Products

Product Documents for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for alpha Tubulin Antibody (YL1/2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

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Protocols

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