Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody - BSA Free [NBP1-80614] -

Knockdown of human XPNPEP1 causes accumulation of DNA damage.(A) Western blot analysis on total protein extracts showing efficient knock down of XPNPEP1 by two independent siRNAs that also induce accumulation of Topoisomerase II alpha. (Β) Pulse field gel stained with ethidium bromide in control cells and following knock down of XPNPEP1. Note increased intensity of band labelled as “a" and the smear label as “b" corresponding to fragmented DNA. Graphs show quantification of the relative intensity of band “a" and smear “b". (C)XPNPEP1 knockdown causes an increase in the % of cells positive for gamma H2AX. Dots show % of cells with >5 gamma H2AX foci from three experiments, bars show mean of the three experiments, and error bars represent standard deviations. Statistical significance was calculated by a one-way ANOVA test, note that differences between siRNA control and the two siRNAs targeting XPNPEP1 are significant (* indicates P value = 0.048 and ** indicates P value = 0.0082). (D) Western blot analysis on fractionated protein extracts from U2OS cells treated with control siRNA and two independent XPNPEP1 siRNAs showing that Topoisomerase II accumulates on the DNA-bound fraction following XPNPEP1 knockdown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35081133), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody - BSA Free [NBP1-80614] -

Knockdown of human XPNPEP1 causes accumulation of DNA damage.(A) Western blot analysis on total protein extracts showing efficient knock down of XPNPEP1 by two independent siRNAs that also induce accumulation of Topoisomerase II alpha. (Β) Pulse field gel stained with ethidium bromide in control cells and following knock down of XPNPEP1. Note increased intensity of band labelled as “a" and the smear label as “b" corresponding to fragmented DNA. Graphs show quantification of the relative intensity of band “a" and smear “b". (C)XPNPEP1 knockdown causes an increase in the % of cells positive for gamma H2AX. Dots show % of cells with >5 gamma H2AX foci from three experiments, bars show mean of the three experiments, and error bars represent standard deviations. Statistical significance was calculated by a one-way ANOVA test, note that differences between siRNA control and the two siRNAs targeting XPNPEP1 are significant (* indicates P value = 0.048 and ** indicates P value = 0.0082). (D) Western blot analysis on fractionated protein extracts from U2OS cells treated with control siRNA and two independent XPNPEP1 siRNAs showing that Topoisomerase II accumulates on the DNA-bound fraction following XPNPEP1 knockdown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35081133), licensed under a CC-BY license. Not internally tested by Novus Biologicals.