AMPK alpha 1 Antibody (RM301)

Name: AMPK alpha 1 Antibody (RM301)
Brand: Novus Biologicals
SKU: NBP2-61491
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-61491

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM301 expressed in HEK293

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Product Specifications

Immunogen

A peptide corresponding to the residues near the C-terminus of Human AMPK alpha 1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK alpha 1 Antibody (RM301)

Western Blot: AMPK alpha 1 Antibody (RM301) [NBP2-61491] -

Western Blot: AMPK alpha 1 Antibody (RM301) [NBP2-61491] - Western Blot of HeLa cells lysate using NBP2-61491 at a 1:100 dilution

Immunohistochemistry: AMPK alpha 1 Antibody (RM301) [NBP2-61491] -

Immunohistochemistry: AMPK alpha 1 Antibody (RM301) [NBP2-61491] - Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue section using NBP2-61491 at a 1:200 dilution

Applications for AMPK alpha 1 Antibody (RM301)

Application

Recommended Usage

Immunohistochemistry

1:100-1:200

Western Blot

1:100 - 1:200

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 Antibody (RM301)

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Certificate of Analysis

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Product Specific Notices for AMPK alpha 1 Antibody (RM301)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Intracellular Kinases

Obesity

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