Anillin Antibody (CL0303) - BSA Free

Novus Biologicals | Catalog # NBP2-52908

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0303

Format

BSA Free
View all Anillin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9NQW6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Anillin Antibody (CL0303) - BSA Free

Western Blot: Anillin Antibody (CL0303) [NBP2-52908]

Western Blot: Anillin Antibody (CL0303) [NBP2-52908]

Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Lane 1: Marker [kDa] Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in U251 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining in human testis and skeletal muscle tissues. Corresponding ANLN RNA-seq data are presented for the same tissues.
Western Blot: Anillin Antibody (CL0303) [NBP2-52908]

Western Blot: Anillin Antibody (CL0303) [NBP2-52908]

Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANLN antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: Anillin Antibody (CL0303) [NBP2-52908]

Western Blot: Anillin Antibody (CL0303) [NBP2-52908]

Western Blot: Anillin Antibody (CL0303) [NBP2-52908] - Interaction of Anillin with GST-fused protein: MDA-MB-231 cells were lysed in TBS, pH 7.5, containing 0.75% Triton X-100 with inhibitors of proteases and phosphatases. Lysate was sonicated and cleared by centrifugation. for the input, sample buffer was added to part of the lysate and heated fro 10min 95 C. The rest of the lysate was incubated with either GST or GST-fused protein immobilized on agarose beads for 2h in 4 C. the beads were washed three times in lysis buffer, resuspended in sample buffer and heated for 10min 95 C. The samples were resolved using SDS-PAGE and immunoblotted with anti-ANLN antibody. This image was submitted by customer review.
Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in A431 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in HeLa cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in MCF7 cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908]

Immunocytochemistry/Immunofluorescence: Anillin Antibody (CL0303) [NBP2-52908] - Staining in U2OS cell line with Anti-ANLN monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human colorectal cancer shows moderate to strong nuclear positivity in a subset of tumor cells.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human rectum shows moderate to strong nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skin shows moderate to strong nuclear positivity in a subset of dermal cells.
Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908]

Immunohistochemistry-Paraffin: Anillin Antibody (CL0303) [NBP2-52908] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.

Applications for Anillin Antibody (CL0303) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-52908 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Anillin

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Alternate Names

actin-binding protein anillin, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Drosophila Scraps homolog, scra, Scraps

Gene Symbol

ANLN

Additional Anillin Products

Product Documents for Anillin Antibody (CL0303) - BSA Free

Certificate of Analysis

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Product Specific Notices for Anillin Antibody (CL0303) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Anillin Antibody (CL0303) - BSA Free

Customer Reviews for Anillin Antibody (CL0303) - BSA Free (1)

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  • Anillin Antibody (CL0303)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: GST-pull down assay
    Species: Human
    Verified Customer | Posted 01/15/2018
    Anillin Antibody (CL0303) - BSA Free NBP2-52908

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