Annexin A1 Antibody (CL0199) - BSA Free
Novus Biologicals | Catalog # NBP2-52875
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0199
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P04083, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (86%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Annexin A1 Antibody (CL0199) - BSA Free
![Western Blot: Annexin A1 Antibody (CL0199) [NBP2-52875]](https://resources.rndsystems.com/images/products/Annexin-A1-Antibody-CL0199-Western-Blot-NBP2-52875-img0007.jpg "Western Blot: Annexin A1 Antibody (CL0199) [NBP2-52875]")
Western Blot: Annexin A1 Antibody (CL0199) [NBP2-52875]
Western Blot: Annexin A1 Antibody (CL0199) [NBP2-52875] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ANXA1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining intensity is presented. Loading control: Anti-GAPDH.![Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (CL0199) [NBP2-52875]](https://resources.rndsystems.com/images/products/Annexin-A1-Antibody-CL0199-Immunocytochemistry-Immunofluorescence-NBP2-52875-img0008.jpg "Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (CL0199) [NBP2-52875]")
Immunocytochemistry/ Immunofluorescence: Annexin A1 Antibody (CL0199) [NBP2-52875]
Immunocytochemistry/Immunofluorescence: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of A549 cells using the Anti-ANXA1 monoclonal antibody, showing specific staining in the nucleus, cytosol and the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]](https://resources.rndsystems.com/images/products/Annexin-A1-Antibody-CL0199-Immunohistochemistry-Paraffin-NBP2-52875-img0005.jpg "Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]")
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of human placenta shows strong immunoreactivity in the trophoblast cells.![Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]](https://resources.rndsystems.com/images/products/Annexin-A1-Antibody-CL0199-Immunohistochemistry-Paraffin-NBP2-52875-img0001.jpg "Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]")
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of human bone marrow shows strong nuclear immunoreactivity in a subset of lymphoid cells.![Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]](https://resources.rndsystems.com/images/products/Annexin-A1-Antibody-CL0199-Immunohistochemistry-Paraffin-NBP2-52875-img0002.jpg "Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]")
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of human bronchus shows distinct positivity in ciliated epithelial cells.![Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]](https://resources.rndsystems.com/images/products/Annexin-A1-Antibody-CL0199-Immunohistochemistry-Paraffin-NBP2-52875-img0003.jpg "Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]")
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of human fallopian tube shows strong positivity in the glandular cells.![Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]](https://resources.rndsystems.com/images/products/Annexin-A1-Antibody-CL0199-Immunohistochemistry-Paraffin-NBP2-52875-img0004.jpg "Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]")
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875]
Immunohistochemistry-Paraffin: Annexin A1 Antibody (CL0199) [NBP2-52875] - Staining of human lung shows nuclear positivity in a subset of pneumocytes and lymphoid cells.Applications for Annexin A1 Antibody (CL0199) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Annexin A1
Alternate Names
ANX1, ANXA1, LPC1
Gene Symbol
ANXA1
Additional Annexin A1 Products
Product Documents for Annexin A1 Antibody (CL0199) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Annexin A1 Antibody (CL0199) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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