Annexin A2 Antibody (1G7) - Azide and BSA Free

Novus Biologicals | Catalog # H00000302-M02

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G7

Format

Azide and BSA Free
View all Annexin A2 Primary Antibodies »

Product Specifications

Immunogen

ANXA2 (AAH66955, 19 a.a. ~ 357 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISVMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28652618).

Specificity

ANXA2 - annexin A2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Annexin A2 Antibody (1G7) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Annexin-A2-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000302-M02-img0007.jpg
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Annexin-A2-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000302-M02-img0008.jpg
Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. ANXA2 Antibody (H00000302-M02) was used for primary antibody: 1:2000, 4C, overnight. WB image submitted by a verified customer review.
Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 monoclonal antibody (M02), clone 1G7 Analysis of ANXA2 expression in HepG2.
Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02]

Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M02), clone 1G7.Lane 1: ANXA2 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02]

Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of monoclonal antibody to ANXA2 on HeLa cell. Antibody concentration 10 ug/ml.
Annexin A2 Antibody (1G7)

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] -

Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 could regulate the activation of NF-kappa B through combined with the subunit p50. a, b. The change in localization of endogenous p50 was analyzed by IF-IC following treatment with or without TNF-alpha (100 ng/ml) for 1 h. Nuclei were stained with DAPI. The nuclear localization of endogenous p50 in each group were quantitated as a graph. The plotted error bars represent mean ± SEM. The nuclear localization of p50 was quantified according to data from 5 random figures in each group. c. Interaction between ANXA2 & p50 in SK-N-BE(2) cells was validated through Co-IP & analyzed by western blotting with the indicated antibodies **: P < 0.01. All results are from at least three independent experiments Image collected & cropped by CiteAb from the following publication (http://jeccr.biomedcentral.com/articles/10.1186/s13046-017-0581-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Annexin A2 Antibody (1G7) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reviewed Applications

Read 1 review rated 5 using H00000302-M02 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Annexin A2

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV

Entrez Gene IDs

302 (Human)

Gene Symbol

ANXA2

OMIM

151740 (Human)

UniProt

AAH66955 (Human)

Additional Annexin A2 Products

Product Documents for Annexin A2 Antibody (1G7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Annexin A2 Antibody (1G7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Annexin A2 Antibody (1G7) - Azide and BSA Free

Customer Reviews for Annexin A2 Antibody (1G7) - Azide and BSA Free (1)

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Customer Images

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  • Annexin A2 Antibody (1G7)
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: MDA-MB-231
    Species: Human
    Verified Customer | Posted 09/12/2021
    Western Blot: MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. ANXA2 Antibody (H00000302-M02) was used for primary antibody: 1:2000, 4℃, overnight.
    Annexin A2 Antibody (1G7) - Azide and BSA Free H00000302-M02

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