Annexin V Antibody (3B9B7) - BSA Free

Novus Biologicals | Catalog # NBP2-52414

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B9B7

Format

BSA Free
View all Annexin V Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Annexin V (AA: 160-320) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Annexin V Antibody (3B9B7) - BSA Free

Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Immunocytochemistry/ Immunofluorescence: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Immunocytochemistry/Immunofluorescence: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - An intracellular stain was performed on HeLa cells with Annexin V Antibody (3B9B7) NBP2-52414AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)
Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7)BSA Free [NBP2-52414]

Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.
Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).
ELISA: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

ELISA: Annexin V Antibody (3B9B7) - BSA Free [NBP2-52414]

ELISA: Annexin V Antibody (3B9B7) [NBP2-52414] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Applications for Annexin V Antibody (3B9B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Annexin V

Annexin V is encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

Alternate Names

ANX5, ANXA5, ANXV

Gene Symbol

ANXA5

Additional Annexin V Products

Product Documents for Annexin V Antibody (3B9B7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Annexin V Antibody (3B9B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Coagulation

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Annexin V Antibody (3B9B7) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?

    A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).

  • Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?

    A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.

  • Q: I am studying apoptosis in adherent cells. Can I trypsinize the cells when using Annexin V-FITC to stain the cells?

    A: Adherent cells undergoing apoptosis automatically detach from the plate and may not require trypsinization. If there are attached cells, they may be trypsinized without affecting phosphatidylserine (PS).

  • Q: I do not have access to flow cytometer in my building. Can I stain for Annexin V and propidium iodide (PI), fix the cells, then analyze them in a different facility?

    A: Fixing the cells can affect cell permeability, enhancing the uptake of Annexin V and PI intracellularly. As a result, the profile of fixed cells may be different from unfixed cells, and the use of appropriate controls is recommended.

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